Mosquito embryos and eggs: polarity and terminology of chorionic layers.

Abstract:

:The development of genetically modified vectors refractory to parasites is seen as a promising strategy in the future control of endemic diseases such as malaria. Nevertheless, knowledge of mosquito embryogenesis, a pre-requisite to the establishment of transgenic individuals, has been presently neglected. We have here studied the eggs from two neotropical malaria vectors. Eggs from Anopheles (Nyssorhynchus) albitarsis and Anopheles (Nyssorhynchus) aquasalis were analyzed by laser scanning microscopy and scanning electron microscopy and compared to those of Drosophila melanogaster. We verified basic conflicting data such as mosquito egg polarity and ultrastructure of eggshell layers. A 180 degrees rotation movement of the mosquito embryo along its longitudinal axis, a phenomenon not conserved among all Diptera, was confirmed. This early event is not taken into account by several present groups, leading to a non-consensual assignment of eggshell dorsal and ventral poles. Since embryo and egg polarities, defined during oogenesis, are the same, we propose to consider the flattened egg side as the dorsal one. The structure of Anopheles eggshell was also examined. Embryos are covered by a smooth endochorion or inner chorion layer. Outside this coat lies the compound exochorion or outer chorion layer, assembled by a thin basal lamellar layer and external tubercles. The terminology related to eggshell layers is discussed.

journal_name

J Insect Physiol

authors

Valle D,Monnerat AT,Soares MJ,Rosa-Freitas MG,Pelajo-Machado M,Vale BS,Lenzi HL,Galler R,Lima JB

doi

10.1016/s0022-1910(98)00154-1

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

701-708

issue

8

eissn

0022-1910

issn

1879-1611

pii

S0022191098001541

journal_volume

45

pub_type

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