Establishment of an optimized PCR method using sequence-specific primers for screening multiple gene polymorphisms simultaneously.

abstract：:Nasopharyngeal carcinoma (NPC) is a highly metastatic malignancy, which is highly prevalent in Southeast Asia and North Africa. Recent studies implicated the critical role of nemo‑like kinase (NLK) in tumor biology. However, the functional role of NLK in NPC has yet to be elucidated. In the present study, the signific...

journal_title：Molecular medicine reports

authors： Chen S,Ma Z,Chen X,Zhang J

更新日期：2014-07-01 00:00:00

abstract：:Toll‑like receptor 3 (TLR3) can react with double stranded RNA and is involved in the inflammatory response to respiratory syncytial virus (RSV) infection. Also, oxidative stress has been reported to be involved in RSV infection. However, the correlation between oxidative stress and TLR3 activation during RSV infectio...

journal_title：Molecular medicine reports

authors： Wang MM,Lu M,Zhang CL,Wu X,Chen JX,Lv WW,Sun T,Qiu H,Huang SH

更新日期：2018-08-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) has been ranked the second leading cause of cancer‑associated mortality in China and the third leading cause of cancer‑associated mortality worldwide. A number of previous studies investigating SLC44A5 have revealed important biological insight and disease‑specific functions. Therefore, ...

journal_title：Molecular medicine reports

authors： Peng GZ,Ye QF,Wang R,Li MX,Yang ZX

更新日期：2016-06-01 00:00:00

abstract：:The present study investigated the role of microRNA (miR)‑146a in a diabetic nephropathy (DN) model, and its molecular mechanism. DN mice were given intraperitoneal injections of streptozotocin (55 mg/kg/day) for 5 consecutive days as an in vivo DN model. The HK‑2 human kidney cell line were exposed to 45% D‑glucose a...

journal_title：Molecular medicine reports

authors： Wan RJ,Li YH

更新日期：2018-03-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small non‑coding RNA molecules, which serve an important function in the development of multidrug resistance in cancer through the post‑transcriptional regulation of gene expression and RNA silencing. In the present study, the functional effects of miR‑197 were analyzed in chemo‑resis...

journal_title：Molecular medicine reports

authors： Xiong HL,Zhou SW,Sun AH,He Y,Li J,Yuan X

更新日期：2015-10-01 00:00:00

abstract：:The present study hypothesized that caffeic acid (3,4‑dihydroxycinnamic acid; CaA) may exert antidepressant‑like effects in rats with chronic unpredictable mild stress via epigenetic mechanisms, such as DNA methylation and hydroxymethylation. The chronic unpredictable mild stress (CUMS) model was used to analyze the e...

journal_title：Molecular medicine reports

authors： Hu J,Cao S,Zhang Z,Wang L,Wang D,Wu Q,Li L

更新日期：2020-12-01 00:00:00

abstract：:Ras‑GTPase‑activating protein SH3 domain‑binding protein 1 (G3BP1) has been reported to be of importance in the occurrence and development of colon cancer. However, the underlying mechanisms remain largely unknown. Therefore, the aim of the present study was to investigate the role of Wnt/β‑catenin signaling in G3BP1‑...

journal_title：Molecular medicine reports

authors： Li Y,Wang J,Zhong S,Li J,Du W

更新日期：2020-11-01 00:00:00

abstract：:In order to observe the effects of cyclin E gene silencing by small interfering RNA (siRNA) on the growth, proliferation, invasion and apoptosis of esophageal cancer cell lines, including EC9706, Eca109 and KYSE30, siRNA vectors targeting cyclin E gene were constructed and then tran...

journal_title：Molecular medicine reports

authors： Wang N,Li M,Zang WQ,Ma YY,Wang YY,Zhao GQ

更新日期：2013-03-01 00:00:00

abstract：:Mucin is produced and secreted by epithelial goblet cells and is a key component of the innate immune system, acting as a barrier in the intestinal tract. However, no studies have been conducted investigating the increase in mucin secretion to enhance the intestinal barrier function. The present study investigated whe...

journal_title：Molecular medicine reports

authors： Yasuda-Onozawa Y,Handa O,Naito Y,Ushiroda C,Suyama Y,Toyokawa Y,Murakami T,Yasuda T,Ueda T,Majima A,Hotta Y,Doi T,Tanaka M,Horii Y,Higashimura Y,Mizushima K,Morita M,Uehara Y,Horie H,Fukui A,Dohi O,Okayama T,Y

更新日期：2017-12-01 00:00:00

abstract：:Mineralized bone matrix constituted with collagenous and non-collagenous proteins was synthesized by osteoblasts differentiated from mesenchymal stem cells. Secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, promotes cell migration and proliferation, and suppresses the inflammatory response. R...

journal_title：Molecular medicine reports

authors： Choi BD,Lee SY,Jeong SJ,Lim DS,Cha HJ,Chung WG,Jeong MJ

更新日期：2016-08-01 00:00:00

abstract：:Chronic bacterial prostatitis (CBP) is a persistent infection of the prostate characterized by poor quality of life mainly due to frequent relapse episodes caused by incomplete eradication of causative pathogens. Aggressive antibacterial therapy is required to attenuate the severe symptoms of CBP and to achieve a perm...

journal_title：Molecular medicine reports

authors： Perletti G,Skerk V,Magri V,Markotic A,Mazzoli S,Parnham MJ,Wagenlehner FM,Naber KG

更新日期：2011-11-01 00:00:00

abstract：:The β1‑adrenergic receptor (AR) is the primary β‑AR subtype in the heart and is the target of metoprolol (Met), which is commonly used to treat angina and hypertension. Previous studies have revealed a positive correlation between the methylation levels of the adrenoreceptor β1 gene (Adrb1) promoter in the myocardium ...

journal_title：Molecular medicine reports

authors： Huang Y,Liu XL,Wen J,Huang LH,Lu Y,Miao RJ,Liu X,Li Y,Xing XW,Yuan H

更新日期：2017-02-01 00:00:00

abstract：:Although the etiology of intervertebral disc degeneration is poorly understood, one approach to prevent this process may be to inhibit apoptosis. In the current study, the anti‑apoptotic effects of carboxymethylated chitosan (CMCS) in nucleus pulposus (NP) cells were investigated with the aim to enhance disc cell surv...

journal_title：Molecular medicine reports

authors： He B,Tao H,Liu S,Wei A

更新日期：2015-03-01 00:00:00

abstract：:Accumulating evidence suggests that Rho-associated kinase (ROCK) may be important in the pathogenesis of atherosclerosis and coronary vasospasm. In the present study, we investigated whether ROCK activity is increased in acute coronary syndrome (ACS) patients. Twenty-one patients with ACS (12 males, mean age 58.0±8.0 ...

journal_title：Molecular medicine reports

authors： Hou HW,Li XG,Yan M,Hu ZQ,Song YE

更新日期：2013-07-01 00:00:00

abstract：:Androgens are essential for the development of the prostate and prostate cancer. We examined androgen-regulated gene expression in the human prostate. Samples from benign and malignant prostate tissue and samples containing prostate tissue obtained from prostate cancer patients thre...

journal_title：Molecular medicine reports

authors： Vaarala MH,Hirvikoski P,Kauppila S,Paavonen TK

更新日期：2012-09-01 00:00:00

abstract：:Macrophages can be polarized into the inflammatory M1 lineage or the immunomodulatory M2 lineage, depending on the differential tissue microenvironment signaling, specific pathogens or cytokine stimulation. Tumor necrosis factor α‑induced protein 8‑like protein 2 (TIPE2) has been demonstrated to negatively regulate in...

journal_title：Molecular medicine reports

authors： Jiang Y,Li Q,Zhang Y,Gao Y,Jiang L,Chen Z

更新日期：2018-01-01 00:00:00

abstract：:There is much interest in the potential application of vector-induced gene therapeutic approaches to several forms of hearing disorders due to the poor efficacy of existing treatments. The cochlea is an ideal site for local gene transfection due to its anatomical encapsulation and fluid flow within its ducts. However,...

journal_title：Molecular medicine reports

authors： Xia L,Yin S

更新日期：2013-07-01 00:00:00

abstract：:Systemic lupus erythematosus is a connective tissue disease characterized by autoimmune inflammation, which leads to specific and nonspecific immune disorders with the formation of various autoantibodies by activated B cells. B‑cell‑activating factor (BAFF) is secreted by macrophages and activated T cells, and is resp...

journal_title：Molecular medicine reports

authors： Ge F,Wang F,Yan X,Li Z,Wang X

更新日期：2017-11-01 00:00:00

abstract：:Lipopolysaccharide (LPS), the major outer surface membrane component of Gram-negative bacteria, is one of the main etiological factors in the pathogenesis of several lung diseases, such as chronic obstructive pulmonary disease. The respiratory epithelium and the macrophages comprise the dynamic interface between the o...

journal_title：Molecular medicine reports

authors： Liu X,Yin S,Chen Y,Wu Y,Zheng W,Dong H,Bai Y,Qin Y,Li J,Feng S,Zhao P

更新日期：2018-04-01 00:00:00

abstract：:Cisplatin is a first generation platinum‑based chemotherapeutic agent, however, the extensive application of cisplatin inevitably results in drug resistance, which is a major obstacle in cancer chemotherapy. The aim of the present study was to investigate the efficiency of reversing cisplatin‑resistance with the use o...

journal_title：Molecular medicine reports

authors： Ma S,Tan W,Du B,Liu W,Li W,Che D,Zhang G

更新日期：2016-04-01 00:00:00

abstract：:The purpose of the present study was to investigate the effect of bufadienolides on the A‑375 melanoma cell line, and to delineate the underlying mechanism. A Cell Counting Kit‑8 assay was used to determine the viability of the cells, and flow cytometry was used to evaluate apoptosis. Western blot analysis was used to...

journal_title：Molecular medicine reports

authors： Li H,Cao X,Chen X,Yi X,Xia J,Chen J,Yang L

更新日期：2019-09-01 00:00:00

abstract：:The biochemical events involved in the upregulation of selected glucose‑responsive genes by 3‑O‑methyl‑D‑glucose (3‑MG) remain to be elucidated. The present study mainly aimed to re‑evaluate the possible role of 3‑MG phosphorylation in the upregulation of the thioredoxin interacting protein (TXNIP) and liver pyruvate ...

journal_title：Molecular medicine reports

authors： Svoboda M,Tastenoy M,Zhang Y,Gillet C,Rasschaert J,Malaisse WJ,Sener A

更新日期：2013-09-01 00:00:00

abstract：:Extracellular potassium homeostasis is dependent on the activity of potassium channels, which are expressed on the apical membrane of epithelial tubular cells. The renal outer medullary potassium channel (ROMK) is considered to be the major route for potassium transport into the tubule lumen. Hepatocyte growth factor ...

journal_title：Molecular medicine reports

authors： Kato T,Oka K,Nakamura T

更新日期：2018-01-01 00:00:00

abstract：:Salicylate is widely used to produce animal models of tinnitus in mice and/or rats. The side effects on auditory function, including hearing loss and tinnitus, are considered the results of the auditory nerve dysfunction. A recent study indicated that chronic treatment with salicylate for several weeks reduces compres...

journal_title：Molecular medicine reports

authors： Cui W,Wang H,Cheng Y,Ma X,Lei Y,Ruan X,Shi L,Lv M

更新日期：2019-01-01 00:00:00

abstract：:It has been suggested that survivin, a member of the inhibitor of apoptosis protein (IAP) family, is an attractive novel target for anticancer therapy. The present study investigated the expression of survivin during the early stages of hepatocellular carcinoma (HCC) and associated hepatitis, and attempted to elucidat...

journal_title：Molecular medicine reports

authors： Mamori S,Matsushima M,Matsuura T,Tajiri H

更新日期：2009-11-01 00:00:00

abstract：:Quantitative polymerase chain reaction (qPCR) analysis is a commonly used method for the study of mRNA expression throughout the field of mesenchymal stem cell (MSC) research. This technology is simple and sensitive; however the results may vary significantly due to the use of various reference genes (RGs) as normaliz...

journal_title：Molecular medicine reports

authors： Li X,Yang Q,Bai J,Yang Y,Zhong L,Wang Y

更新日期：2015-02-01 00:00:00

abstract：:Triple‑negative breast cancer (TNBC) is a heterogeneous disease characterized by an aggressive phenotype and reduced survival. The aim of the present study was to investigate the molecular mechanisms involved in the carcinogenesis of TNBC and to identify novel target molecules for therapy. The differentially expressed...

journal_title：Molecular medicine reports

authors： Peng C,Ma W,Xia W,Zheng W

更新日期：2017-03-01 00:00:00

abstract：:Infantile hemangioma (IH) is one of the most common vascular tumors that occurs during childhood, but its pathogenesis is currently not completely understood. Even though lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays vital roles in tumorigenesis of malignant tumors, its roles in IH remain unclear. The...

journal_title：Molecular medicine reports

authors： Yu L,Shu H,Xing L,Lv MX,Li L,Xie YC,Zhang Z,Zhang L,Xie YY

更新日期：2020-10-01 00:00:00

abstract：:Rutin has extensive pharmacological activities, including antibacterial and anti-inflammatory activities, cooling of the blood to inhibit bleeding, reducing capillary wall fragility and anti-influenza activities. However, whether rutin can ameliorate neuropathic function in spinal cord injury (SCI) in constriction-ind...

journal_title：Molecular medicine reports

authors： Zhang P,Ma X

更新日期：2015-11-01 00:00:00

abstract：:Wilms tumor (WT) is the most common type of renal malignancy in children. Survival rates are low and high‑risk WT generally still carries a poor prognosis. To better elucidate the pathogenesis and tumorigenic pathways of high‑risk WT, the present study presents an integrated analysis of RNA expression profiles of high...

journal_title：Molecular medicine reports

authors： Wang X,Song P,Huang C,Yuan N,Zhao X,Xu C

更新日期：2019-03-01 00:00:00