Identification of carriers among individuals recruited in the typhoid registry in Malaysia using stool culture, polymerase chain reaction, and dot enzyme immunoassay as detection tools.

Abstract:

:Chronic carriers of Salmonella Typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, designated Typhidot-C, was evaluated in retrospect using the detected individuals as control positives. Chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 out of 110) among individuals who previously had acute typhoid fever and a 9.4% (10 out of 106) carriage rate was observed among food handlers screened during outbreaks. The Typhidot-C assay was able to detect all these positive carriers showing its potential as a viable carrier screening tool and can be used for efficient detection of typhoid carriers in an endemic area. These findings were used to establish the first carrier registry for S Typhi carriers in Malaysia.

authors

Chua AL,Aziah I,Balaram P,Bhuvanendran S,Anthony AA,Mohmad SN,Nasir NM,Hassan H,Naim R,Meran LP,Hussin HM,Ismail A

doi

10.1177/1010539512458521

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

NP2740-8

issue

2

eissn

1010-5395

issn

1941-2479

pii

1010539512458521

journal_volume

27

pub_type

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