Abstract:
:Desmosomes are intercellular junctions that confer strong cell-cell adhesion. Altered expression of desmocollin 3 (DSC3), a member of the desmosomal cadherin family, was found in various cancers; however, its functional involvement in carcinogenesis has not yet been elucidated. Expression/localization of DSC3 was analyzed by real-time reverse transcription-PCR, western blotting, immunofluorescence and immunohistochemistry. Methylation status of DSC3 was examined by demethylation tests, methylation-specific PCR and bisulfite sequencing. It turned out that downregulation of DSC3 in lung cancer cells was associated with DNA hypermethylation. In primary lung tumors, DSC3 was a potential diagnostic marker for lung squamous cell carcinoma, and DSC3 DNA hypermethylation was correlated with poor clinical outcome. To investigate the effect of the tumor suppressor gene p53 on DSC3, transient transfection with a wild-type p53-expression vector was performed. Overexpression of p53 resulted in an increased expression of DSC3 in a DSC3-unmethylated lung cancer cell line H2170, but not in H1299, a DSC3-methylated cell line. However, combination of p53 transfection with demethylation agent 5-aza-2'-deoxycytidine treatment led to increased expression of DSC3 in H1299 cells. Furthermore, functional studies after stable transfection of a DSC3 expression vector showed that ectopic expression of DSC3 inhibited cell proliferation, anchorage-independent growth, migration, as well as invasion, and most interestingly led to reduced phosphorylation levels of extracellular signal-regulated kinase1/2. Taken together, our data suggested that DSC3 acts as a novel tumor suppressor gene through inhibition of epidermal growth factor receptor/extracellular signal-regulated kinase signaling in lung cancer cells.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Cui T,Chen Y,Yang L,Knösel T,Huber O,Pacyna-Gengelbach M,Petersen Idoi
10.1093/carcin/bgs273subject
Has Abstractpub_date
2012-12-01 00:00:00pages
2326-33issue
12eissn
0143-3334issn
1460-2180pii
bgs273journal_volume
33pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::The repair kinetics and sensitivity to inhibitors of DNA strand breaks caused by superoxide anion, hydrogen peroxide, benzoyl peroxide and anthralin have been studied and compared with strand breaks caused by well-studied agents such as ionizing radiation and bleomycin. The latter two agents are generally believed to ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.9.1511
更新日期:1986-09-01 00:00:00
abstract::A single i.p. injection of 12-O-tetradecanoylphorbol-13-acetate (TPA) induced a transient increase in the levels of rat liver putrescine, spermidine and spermine. These polyamine concentrations continuously increased until 6 h, immediately following administration of the tumor promoter. Phenobarbital (PB) induced an i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.2.225
更新日期:1984-02-01 00:00:00
abstract::The effect of retinoids on the induction of both epithelial and mesenchymal tumors of the rat kidney by a single dose of 40 mg/kg dimethylnitrosamine was tested using 13-cis-retinoic acid and the trimethylmethoxy phenyl analog of retinoic acid ethylamide. 13-cis-retinoic acid was administered for 3 weeks, 10 weeks, or...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.5.665
更新日期:1984-05-01 00:00:00
abstract::MicroRNAs (miRNAs) are a class of short non-coding, single-stranded RNAs, which perform posttranscriptional regulatory functions as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) genes are currently being identified for contributing to cancer risk, prognosis and survival. ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu082
更新日期:2014-07-01 00:00:00
abstract::Metabolism and macromolecular interaction of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) were studied in human buccal mucosa in vitro. Microautoradiographic analysis of [5-3H]NNK-exposed explant cultures demonstrated a uniform distribution of bound radioactivity in the mucosal ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.11.2383
更新日期:1993-11-01 00:00:00
abstract::In addition to genetic changes, epigenetic aberrations also play important roles in radiation- and chemical-induced disorders and carcinogenesis. The present study investigated whether epigenetic therapy with a histone deacetylase (HDAC) inhibitor has dual benefits for radiation-induced oral mucositis and chemical-ind...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp079
更新日期:2009-08-01 00:00:00
abstract::Monoclonal or affinity-purified antibodies specific to Novikoff hepatoma cytokeratin p39 were employed to study the origin and fate of p39-containing cell types during hepatocarcinogenesis induced with N,N-dimethyl-p(m-tolylazo)aniline. Frozen sections were obtained from the livers of animals autopsied temporally duri...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.8.1147
更新日期:1985-08-01 00:00:00
abstract::The mutagen sensitivity assay is an in vitro measure of DNA repair capacity used to evaluate intrinsic susceptibility for cancer. The high heritability of mutagen sensitivity to different mutagens validates the use of this phenotype to predict cancer susceptibility. However, genetic determinants of mutagen sensitivity...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn121
更新日期:2008-07-01 00:00:00
abstract::Transgenic mice containing one copy of hepatitis B virus (HBV) genome without the core gene and expressing hepatitis B surface antigen (HBsAg) were crossed with C3H/He mice. The F1 hybrids (approximately 50% HBV positive and approximately 50% HBV negative) were treated with a single dose of diethylnitrosamine (NDEA) o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.6.953
更新日期:1990-06-01 00:00:00
abstract::High intake of red meat, but not of white meat, is associated with an increased risk of colon cancer. However, red meat does not promote cancer in rodents. Haemin, added to low-calcium diets, increases colonic proliferation, and haemoglobin, added to high-fat diets, increases the colon tumour incidence in rats, an eff...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg130
更新日期:2003-10-01 00:00:00
abstract::Arsenicals are epidemiologically significant chemicals in relation to induction of urinary bladder cancer in man. In the present study, we investigated the dose-dependent promotion potential of dimethylarsinic acid (DMA), a major metabolite of inorganic arsenicals in mammals, for rat urinary bladder carcinogenesis. In...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.11.2435
更新日期:1996-11-01 00:00:00
abstract::In order to develop more efficient in vitro systems for the study of pro-mutagenic or pro-carcinogenic chemicals, we have produced transgenic C3H/10T1/2 cell lines expressing human cytochrome P450 (CYP) 2A6. A retroviral vector containing the cDNA was packaged in psi-2 cells, and used to infect C3H/10T1/2 cells. From ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.7.1421
更新日期:1993-07-01 00:00:00
abstract::An earlier study indicated that ozone (O3), a major pollutant in our atmosphere, acts as a carcinogen as well as a synergistic co-carcinogen with radiation in cultured hamster embryo cells and in mouse C3H10T1/2 cells. In this investigation we further characterize the oncogenic action of ozone, alone or in combination...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.8.1549
更新日期:1989-08-01 00:00:00
abstract::The suppression of apoptosis may contribute to the carcinogenicity of the peroxisome proliferators (PPs), a class of non-genotoxic rodent hepatocarcinogens. Our previous work demonstrated that the PP nafenopin suppressed both spontaneous and transforming growth factor beta1 (TGFbeta1)-induced hepatocyte apoptosis both...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.2.299
更新日期:1998-02-01 00:00:00
abstract::To establish a cell culture model for lung carcinogenesis, independent populations of the human papillomavirus 18-immortalized human bronchial epithelial cell line BEP2D were treated with high linear energy transfer radon-simulated alpha-particles, expanded and xenotransplanted into Nu/Nu mice. Six independent cell li...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.6.1251
更新日期:1997-06-01 00:00:00
abstract::Genetically based differences in metabolism, related to MspI restriction site and Ile-Val polymorphisms of the cytochrome P450 (CYP) 1A1 gene and the null genotype of glutathione transferase class mu (GSTM1), have been reported to be associated with lung cancer susceptibility. The present study was set up to establish...
journal_title:Carcinogenesis
pub_type: 杂志文章,多中心研究
doi:10.1093/carcin/15.9.1785
更新日期:1994-09-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA), a major adrenal secretory product in men and women, is a potent inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH). Long-term treatment with this steroid has previously been found to suppress spontaneous breast cancer development in C3H mice. DHEA is now shown to inhibit Ep...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.7.683
更新日期:1981-01-01 00:00:00
abstract::Both Snell dwarf mice (dw/dw) and their phenotypically normal heterozygotes (dw/+) were dosed with methylclofenapate (MCP) at daily intervals by gavage (25 mg/kg). Animals were killed at 12, 24, 36 and 72 h after the initial dose and the parameters of ploidy, nuclearity and DNA synthesis were measured in hepatocytes i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.3.387
更新日期:1990-03-01 00:00:00
abstract::We previously demonstrated that fasting/refeeding enhances the initiation phase of liver and colorectal carcinogenesis in rats. The present study was undertaken to establish whether cycles of fasting/refeeding carried out during the promotion phase of carcinogenesis may also affect the formation of aberrant crypt foci...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.2.323
更新日期:2002-02-01 00:00:00
abstract::Recent studies have revealed that 1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG) has anti-tumorigenic activity in vitro. In the present work, we evaluated the in vitro and in vivo antiangiogenic and antitumor activities of PGG and examined its molecular mechanisms. PGG significantly inhibited the proliferation and tub...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi097
更新日期:2005-08-01 00:00:00
abstract::Experiments were conducted to determine whether the natural estrogen and an environmental compound with estrogenic action, 4-n-octylphenol (4nOP), could modify tumor development in human c-Ha-ras proto-oncogene transgenic (Tg) rats which are highly susceptible to mammary and skin carcinogens. Female and male Tg and no...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.7.1209
更新日期:2002-07-01 00:00:00
abstract::We have previously demonstrated that the active dihydrofolate reductase (DHFR) gene is efficiently repaired in Chinese hamster ovary (CHO) cells which remove only a small fraction of u.v.-induced pyrimidine dimers from the overall genome. Preferential DNA repair of essential genes may explain why the u.v. resistance o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1333
更新日期:1987-09-01 00:00:00
abstract::The transforming potential of acrylonitrile epoxide (ANO) was tested in a modified NIH3T3 transfection-transformation assay. This involves a new ras construct obtained by ligating a human c-Ha-ras-1 proto-oncogene to the pSV2neo mammalian vector. The new plasmid was allowed to react with ANO or an established carcinog...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.5.787
更新日期:1991-05-01 00:00:00
abstract::Weanling male F344 rats were fed either a semi-purified diet low in methionine and lacking in choline and folic acid (folate/methyl deficient) or a supplemented control diet for periods of 2, 5, 7 days, 3 weeks, and 9 weeks. Two days after initiating the folate/methyl deficient diet in weanling F344 rats, the incidenc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.2.287
更新日期:1997-02-01 00:00:00
abstract::The 14-3-3 proteins are associated with proto-oncogene and oncogene products. Here, we generated NIH 3T3 cells overexpressing the beta isoform of the 14-3-3 proteins (14-3-3 beta) to examine the function of this isoform in cellular proliferation and oncogenic transformation. Overexpression of 14-3-3 beta in NIH 3T3 ce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.11.2073
更新日期:2000-11-01 00:00:00
abstract::Two complementary genetic parameters were followed in liver parenchymal cells during the first steps of rat hepatocarcinogenesis: the expression of nucleolar genes estimated from their silver stainability and the nuclear DNA content determined after Feulgen staining. Putative preneoplastic lesions as foci and nodules ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.2.195
更新日期:1987-02-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most common cancer worldwide. Chronic inflammation appears to enhance the risk of CRC. Emerging evidence has suggested that epigenetic mechanisms play an important role in CRC. Aspirin [acetylsalicylic acid (ASA)] has been shown to prevent CRC; however, the epigenetic mechanisms of...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw042
更新日期:2016-06-01 00:00:00
abstract::Gangliosides from liver parenchymal and non-parenchymal cells were isolated from Fischer 344 rats that had been fed normal diet or a diet supplemented with 0.03% N-2-acetylaminofluorene (AAF) for 4 weeks. Gangliosides from liver cell fractions were characterized by an induction of both II3NeuAcIV3 alpha GalIV2FucGg4 a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.1.89
更新日期:1990-01-01 00:00:00
abstract::Diethylstilbestrol (DES), a synthetic stilbene estrogen, is a potent development toxin and carcinogen in humans and rodents. A number of 32P-postlabeling studies suggest that genotoxic effects of DES substantially contribute to these biological effects. The mechanisms involved in DES-mediated genotoxicity are not comp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.11.2643
更新日期:1995-11-01 00:00:00
abstract::Benzidine diimine (BZDI), a reactive intermediate in the metabolic peroxidation of the carcinogen benzidine, has been reported to bind covalently to cellular proteins and nucleic acids. We have examined the nature of this interaction with DNA and have identified a major carcinogen-nucleoside adduct. BZDI (20-50 microM...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.1.179
更新日期:1986-01-01 00:00:00