Abstract:
:We have taken an engineering approach to extending the lifespan of Caenorhabditis elegans. Aging stands out as a complex trait, because events that occur in old animals are not under strong natural selection. As a result, lifespan can be lengthened rationally using bioengineering to modulate gene expression or to add exogenous components. Here, we engineered longer lifespan by expressing genes from zebrafish encoding molecular functions not normally present in worms. Additionally, we extended lifespan by increasing the activity of four endogenous worm aging pathways. Next, we used a modular approach to extend lifespan by combining components. Finally, we used cell- and worm-based assays to analyze changes in cell physiology and as a rapid means to evaluate whether multi-component transgenic lines were likely to have extended longevity. Using engineering to add novel functions and to tune endogenous functions provides a new framework for lifespan extension that goes beyond the constraints of the worm genome.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Sagi D,Kim SKdoi
10.1371/journal.pgen.1002780subject
Has Abstractpub_date
2012-01-01 00:00:00pages
e1002780issue
6eissn
1553-7390issn
1553-7404pii
PGENETICS-D-12-00395journal_volume
8pub_type
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