Abstract:
:When animals move, respiration increases to adapt for increased energy demands; the underlying mechanisms are still not understood. We investigated the neural substrates underlying the respiratory changes in relation to movement in lampreys. We showed that respiration increases following stimulation of the mesencephalic locomotor region (MLR) in an in vitro isolated preparation, an effect that persists in the absence of the spinal cord and caudal brainstem. By using electrophysiological and anatomical techniques, including whole-cell patch recordings, we identified a subset of neurons located in the dorsal MLR that send direct inputs to neurons in the respiratory generator. In semi-intact preparations, blockade of this region with 6-cyano-7-nitroquinoxaline-2,3-dione and (2R)-amino-5-phosphonovaleric acid greatly reduced the respiratory increases without affecting the locomotor movements. These results show that neurons in the respiratory generator receive direct glutamatergic connections from the MLR and that a subpopulation of MLR neurons plays a key role in the respiratory changes linked to movement.
journal_name
Proc Natl Acad Sci U S Aauthors
Gariépy JF,Missaghi K,Chevallier S,Chartré S,Robert M,Auclair F,Lund JP,Dubuc Rdoi
10.1073/pnas.1113002109subject
Has Abstractpub_date
2012-01-10 00:00:00pages
E84-92issue
2eissn
0027-8424issn
1091-6490pii
1113002109journal_volume
109pub_type
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