Cell dedifferentiation and epithelial to mesenchymal transitions during intestinal regeneration in H. glaberrima.


BACKGROUND:Determining the type and source of cells involved in regenerative processes has been one of the most important goals of researchers in the field of regeneration biology. We have previously used several cellular markers to characterize the cells involved in the regeneration of the intestine in the sea cucumber Holothuria glaberrima. RESULTS:We have now obtained a monoclonal antibody that labels the mesothelium; the outer layer of the gut wall composed of peritoneocytes and myocytes. Using this antibody we studied the role of this tissue layer in the early stages of intestinal regeneration. We have now shown that the mesothelial cells of the mesentery, specifically the muscle component, undergo dedifferentiation from very early on in the regeneration process. Cell proliferation, on the other hand, increases much later, and mainly takes place in the mesothelium or coelomic epithelium of the regenerating intestinal rudiment. Moreover, we have found that the formation of the intestinal rudiment involves a novel regenerative mechanism where epithelial cells ingress into the connective tissue and acquire mesenchymal phenotypes. CONCLUSIONS:Our results strongly suggest that the dedifferentiating mesothelium provides the initial source of cells for the formation of the intestinal rudiment. At later stages, cell proliferation supplies additional cells necessary for the increase in size of the regenerate. Our data also shows that the mechanism of epithelial to mesenchymal transition provides many of the connective tissue cells found in the regenerating intestine. These results present some new and important information as to the cellular basis of organ regeneration and in particular to the process of regeneration of visceral organs.


BMC Dev Biol


García-Arrarás JE,Valentín-Tirado G,Flores JE,Rosa RJ,Rivera-Cruz A,San Miguel-Ruiz JE,Tossas K




Has Abstract


2011-10-17 00:00:00










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    pub_type: 杂志文章


    authors: Mourikis P,Tajbakhsh S

    更新日期:2014-01-24 00:00:00

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    pub_type: 杂志文章


    authors: Teixeira V,Arede N,Gardner R,Rodríguez-León J,Tavares AT

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    authors: Bensimon-Brito A,Cardeira J,Cancela ML,Huysseune A,Witten PE

    更新日期:2012-10-09 00:00:00

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    pub_type: 杂志文章


    authors: Wang Z,Huang Y,Tan L

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    pub_type: 杂志文章


    authors: LaLonde M,Janssens H,Yun S,Crosby J,Redina O,Olive V,Altshuller YM,Choi SY,Du G,Gergen JP,Frohman MA

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    authors: King RS,Newmark PA

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    pub_type: 杂志文章


    authors: Kallio H,Pastorekova S,Pastorek J,Waheed A,Sly WS,Mannisto S,Heikinheimo M,Parkkila S

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    authors: Peel AD,Schanda J,Grossmann D,Ruge F,Oberhofer G,Gilles AF,Schinko JB,Klingler M,Bucher G

    更新日期:2013-06-18 00:00:00

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    authors: Schotthöfer SK,Bohrmann J

    更新日期:2020-07-08 00:00:00

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    pub_type: 杂志文章


    authors: Yan L,Carr J,Ashby PR,Murry-Tait V,Thompson C,Arthur JS

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    pub_type: 杂志文章


    authors: Smith TS,Pineda JM,Donaghy AC,Damer CK

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    pub_type: 杂志文章


    authors: French CR,Erickson T,Callander D,Berry KM,Koss R,Hagey DW,Stout J,Wuennenberg-Stapleton K,Ngai J,Moens CB,Waskiewicz AJ

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    authors: Cash AC,Andrews J

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    authors: Harpavat S,Cepko CL

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    abstract:BACKGROUND:MiR-9 is a small non-coding RNA that is highly conserved between species and primarily expressed in the central nervous system (CNS). It is known to influence proliferation and neuronal differentiation in the brain and spinal cord of different vertebrates. Different studies have pointed to regional and speci...

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    authors: Alwin Prem Anand A,Huber C,Asnet Mary J,Gallus N,Leucht C,Klafke R,Hirt B,Wizenmann A

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    abstract:BACKGROUND:Super-resolution fluorescence microscopy performed via 3D structured illumination microscopy (3D-SIM) is well established on flat, adherent cells. However, blastomeres of mammalian embryos are non-adherent, round and large. Scanning whole mount mammalian embryos with 3D-SIM is prone to failure due to the mov...

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    authors: Popken J,Dahlhoff M,Guengoer T,Wolf E,Zakhartchenko V

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    abstract:BACKGROUND:Circadian clocks have been implicated in the regulation of pre-adult development of fruit flies Drosophila melanogaster. It is believed that faster clocks speed up development and slower clocks slow it down. We established three sets of D. melanogaster populations (early, control and late). The early and lat...

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    authors: Kumar S,Vaze KM,Kumar D,Sharma VK

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  • Regeneration of neural crest derivatives in the Xenopus tadpole tail.

    abstract:BACKGROUND:After amputation of the Xenopus tadpole tail, a functionally competent new tail is regenerated. It contains spinal cord, notochord and muscle, each of which has previously been shown to derive from the corresponding tissue in the stump. The regeneration of the neural crest derivatives has not previously been...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Lin G,Chen Y,Slack JM

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  • Activated Ras alters lens and corneal development through induction of distinct downstream targets.

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Burgess D,Zhang Y,Siefker E,Vaca R,Kuracha MR,Reneker L,Overbeek PA,Govindarajan V

    更新日期:2010-01-27 00:00:00

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    pub_type: 杂志文章


    authors: Goossens K,Van Poucke M,Van Soom A,Vandesompele J,Van Zeveren A,Peelman LJ

    更新日期:2005-12-03 00:00:00

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Rappleye CA,Tagawa A,Le Bot N,Ahringer J,Aroian RV

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  • Prolonged FGF signaling is necessary for lung and liver induction in Xenopus.

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    pub_type: 杂志文章


    authors: Shifley ET,Kenny AP,Rankin SA,Zorn AM

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    pub_type: 杂志文章


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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Liu T,Zimmerman KK,Patterson GI

    更新日期:2004-09-20 00:00:00

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Hirose K,Shiomi T,Hozumi S,Kikuchi Y

    更新日期:2014-12-06 00:00:00

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Camarata T,Snyder D,Schwend T,Klosowiak J,Holtrup B,Simon HG

    更新日期:2010-10-15 00:00:00