Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development.


BACKGROUND:The FERM domain containing protein Mosaic Eyes (Moe) interacts with Crumbs proteins, which are important regulators of apical identity and size. In zebrafish, loss-of-function mutations in moe result in defects in brain ventricle formation, retinal pigmented epithelium and neural retinal development, pericardial edema, and tail curvature. In humans and mice, there are two major alternately spliced isoforms of the Moe orthologue, Erythrocyte Protein Band 4.1-Like 5 (Epb4.1l5), which we have named Epb4.1l5long and Epb4.1l5short, that differ after the FERM domain. Interestingly, Moe and both Epb4.1l5 isoforms have a putative C' terminal Type-I PDZ-Binding Domain (PBD). We previously showed that the N' terminal FERM domain in Moe directly mediates interactions with Crumbs proteins and Nagie oko (Nok) in zebrafish, but the function of the C'terminal half of Moe/Epb4.1l5 has not yet been examined. RESULTS:To define functionally important domains in zebrafish Moe and murine Epb4.1l5, we tested whether injection of mRNAs encoding these proteins could rescue defects in zebrafish moe- embryos. Injection of either moe or epb4.1l5long mRNA, but not epb4.1l5short mRNA, could rescue moe- embryonic defects. We also tested whether mRNA encoding C' terminal truncations of Epb4.1l5long or chimeric constructs with reciprocal swaps of the isoform-specific PBDs could rescue moe- defects. We found that injection of the Epb4.1l5short chimera (Epb4.1l5short+long_PBD), containing the PBD from Epb4.1l5long, could rescue retinal and RPE defects in moe- mutants, but not brain ventricle formation. Injection of the Epb4.1l5long chimera (Epb4.1l5long+short_PBD), containing the PBD from Epb4.1l5short, rescued retinal defects, and to a large extent rescued RPE integrity. The only construct that caused a dominant phenotype in wild-type embryos, was Epb4.1l5long+short_PBD, which caused brain ventricle defects and edema that were similar to those observed in moe- mutants. Lastly, the morphology of rod photoreceptors in moe- mutants where embryonic defects were rescued by moe or epb4.1l5long mRNA injection is abnormal and their outer segments are larger than normal. CONCLUSION:Taken together, the data reveal tissue specificity for the function of the PBD in Epb4.1l5long, and suggest that additional C' terminal sequences are important for zebrafish retinal development. Additionally, our data provide further evidence that Moe is a negative regulator of rod outer segment size.


BMC Dev Biol


Christensen AK,Jensen AM




Has Abstract


2008-01-11 00:00:00










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    abstract:BACKGROUND:The neural tube is formed by morphogenetic movements largely dependent on cytoskeletal dynamics. Actin and many of its associated proteins have been proposed as important mediators of neurulation. For instance, mice deficient in MARCKS, an actin cross-linking membrane-associated protein that is regulated by ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Zolessi FR,Arruti C

    更新日期:2001-01-01 00:00:00

  • G1 checkpoint establishment in vivo during embryonic liver development.

    abstract:BACKGROUND:The DNA damage-mediated cell cycle checkpoint is an essential mechanism in the DNA damage response (DDR). During embryonic development, the characteristics of cell cycle and DNA damage checkpoint evolve from an extremely short G1 cell phase and lacking G1 checkpoint to lengthening G1 phase and the establishm...

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    pub_type: 杂志文章


    authors: Wang XQ,Chan KK,Ming X,Lui VC,Poon RY,Lo CM,Norbury C,Poon RT

    更新日期:2014-05-19 00:00:00

  • Dynamics of muscle fibre growth during postnatal mouse development.

    abstract:BACKGROUND:Postnatal growth in mouse is rapid, with total skeletal muscle mass increasing several-fold in the first few weeks. Muscle growth can be achieved by either an increase in muscle fibre number or an increase in the size of individual myofibres, or a combination of both. Where myofibre hypertrophy during growth...

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    pub_type: 杂志文章


    authors: White RB,Biérinx AS,Gnocchi VF,Zammit PS

    更新日期:2010-02-22 00:00:00

  • Activated Ras alters lens and corneal development through induction of distinct downstream targets.

    abstract:BACKGROUND:Mammalian Ras genes regulate diverse cellular processes including proliferation and differentiation and are frequently mutated in human cancers. Tumor development in response to Ras activation varies between different tissues and the molecular basis for these variations are poorly understood. The murine lens...

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    pub_type: 杂志文章


    authors: Burgess D,Zhang Y,Siefker E,Vaca R,Kuracha MR,Reneker L,Overbeek PA,Govindarajan V

    更新日期:2010-01-27 00:00:00

  • Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.

    abstract:BACKGROUND:Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously reported human pancreas organoid (hPO) cultures do not expand efficiently long-term in a chemic...

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    pub_type: 杂志文章


    authors: Georgakopoulos N,Prior N,Angres B,Mastrogiovanni G,Cagan A,Harrison D,Hindley CJ,Arnes-Benito R,Liau SS,Curd A,Ivory N,Simons BD,Martincorena I,Wurst H,Saeb-Parsy K,Huch M

    更新日期:2020-02-26 00:00:00

  • Molecular characterization of retinal stem cells and their niches in adult zebrafish.

    abstract:BACKGROUND:The persistence in adult teleost fish of retinal stem cells that exhibit all of the features of true 'adult stem cells'--self-renewal, multipotency, and the capacity to respond to injury by mitotic activation with the ability to regenerate differentiated tissues--has been known for several decades. However, ...

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    pub_type: 杂志文章


    authors: Raymond PA,Barthel LK,Bernardos RL,Perkowski JJ

    更新日期:2006-07-26 00:00:00

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    abstract:BACKGROUND:Formation of branching tubes is a fundamental step in the development of glandular organs. To identify extracellular cues that orchestrate epithelial tubulogenesis, we employed an in vitro assay in which EpH4-J3B1A mammary epithelial cells form spheroidal cysts when grown in collagen gels under serum-free co...

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    pub_type: 杂志文章


    authors: Montesano R,Carrozzino F,Soulié P

    更新日期:2007-02-08 00:00:00

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    pub_type: 杂志文章


    authors: Weiß I,Bohrmann J

    更新日期:2019-11-12 00:00:00

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    authors: Mashanov VS,Zueva OR,Rojas-Catagena C,Garcia-Arraras JE

    更新日期:2010-11-29 00:00:00

  • Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain.

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Numayama-Tsuruta K,Arai Y,Takahashi M,Sasaki-Hoshino M,Funatsu N,Nakamura S,Osumi N

    更新日期:2010-01-18 00:00:00

  • FGF signaling controls caudal hindbrain specification through Ras-ERK1/2 pathway.

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    pub_type: 杂志文章


    authors: Aragon F,Pujades C

    更新日期:2009-12-03 00:00:00

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    abstract:BACKGROUND:ERK5 is a member of the mitogen activated protein kinase family activated by certain mitogenic or stressful stimuli in cells, but whose physiological role is largely unclear. RESULTS:To help determine the function of ERK5 we have used gene targeting to inactivate this gene in mice. Here we report that ERK5 ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Yan L,Carr J,Ashby PR,Murry-Tait V,Thompson C,Arthur JS

    更新日期:2003-12-16 00:00:00

  • Gene expression studies of developing bovine longissimus muscle from two different beef cattle breeds.

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    pub_type: 杂志文章


    authors: Lehnert SA,Reverter A,Byrne KA,Wang Y,Nattrass GS,Hudson NJ,Greenwood PL

    更新日期:2007-08-16 00:00:00

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    pub_type: 杂志文章


    authors: Holohan EE,zur Lage PI,Jarman AP

    更新日期:2006-11-09 00:00:00

  • Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos.

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    authors: Goossens K,Van Poucke M,Van Soom A,Vandesompele J,Van Zeveren A,Peelman LJ

    更新日期:2005-12-03 00:00:00

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  • Late gestational lung hypoplasia in a mouse model of the Smith-Lemli-Opitz syndrome.

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    pub_type: 杂志文章


    authors: Yu H,Wessels A,Chen J,Phelps AL,Oatis J,Tint GS,Patel SB

    更新日期:2004-02-02 00:00:00

  • Fine scale analysis of gene expression in Drosophila melanogaster gonads reveals Programmed cell death 4 promotes the differentiation of female germline stem cells.

    abstract:BACKGROUND:Germline stem cells (GSCs) are present in the gonads of Drosophila females and males, and their proper maintenance, as well as their correct differentiation, is essential for fertility and fecundity. The molecular characterization of factors involved in maintenance and differentiation is a major goal both in...

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    authors: Cash AC,Andrews J

    更新日期:2012-01-17 00:00:00

  • Expression of carbonic anhydrases IX and XII during mouse embryonic development.

    abstract:BACKGROUND:Of the thirteen active carbonic anhydrase (CA) isozymes, CA IX and XII have been linked to carcinogenesis. It has been suggested that these membrane-bound CAs participate in cancer cell invasion, which is facilitated by an acidic tumor cell environment. Since active cell migration is a characteristic feature...

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    authors: Kallio H,Pastorekova S,Pastorek J,Waheed A,Sly WS,Mannisto S,Heikinheimo M,Parkkila S

    更新日期:2006-05-23 00:00:00

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    abstract:BACKGROUND:The freshwater planarian Schmidtea mediterranea has emerged as a powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical methods for determining gene expression patterns in pla...

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    authors: King RS,Newmark PA

    更新日期:2013-03-12 00:00:00

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    abstract:BACKGROUND:Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS:We describe here involvement of the signaling enzyme Phosp...

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    pub_type: 杂志文章


    authors: LaLonde M,Janssens H,Yun S,Crosby J,Redina O,Olive V,Altshuller YM,Choi SY,Du G,Gergen JP,Frohman MA

    更新日期:2006-12-07 00:00:00

  • Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst.

    abstract:BACKGROUND:Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the bl...

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    authors: Zolini AM,Negrón-Pérez VM,Hansen PJ

    更新日期:2019-07-05 00:00:00

  • Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange.

    abstract:BACKGROUND:Changes in transcellular bioelectrical patterns are known to play important roles during developmental and regenerative processes. The Drosophila follicular epithelium has proven to be an appropriate model system for studying the mechanisms by which bioelectrical signals emerge and act. Fluorescent indicator...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Schotthöfer SK,Bohrmann J

    更新日期:2020-07-08 00:00:00

  • Construction of a cDNA library for miniature pig mandibular deciduous molars.

    abstract:BACKGROUND:The miniature pig provides an excellent experimental model for tooth morphogenesis because its diphyodont and heterodont dentition resembles that of humans. However, little information is available on the process of tooth development or the exact molecular mechanisms controlling tooth development in miniatur...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Song T,Wu T,Wei F,Li A,Wang F,Xie Y,Liu D,Fan Z,Wang X,Cheng S,Zhang C,He J,Wang S

    更新日期:2014-04-21 00:00:00

  • myGRN: a database and visualisation system for the storage and analysis of developmental genetic regulatory networks.

    abstract:BACKGROUND:Biological processes are regulated by complex interactions between transcription factors and signalling molecules, collectively described as Genetic Regulatory Networks (GRNs). The characterisation of these networks to reveal regulatory mechanisms is a long-term goal of many laboratories. However compiling, ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Bacha J,Brodie JS,Loose MW

    更新日期:2009-06-06 00:00:00

  • Knockout of the folate transporter folt-1 causes germline and somatic defects in C. elegans.

    abstract:BACKGROUND:The C. elegans gene folt-1 is an ortholog of the human reduced folate carrier gene. The FOLT-1 protein has been shown to transport folate and to be involved in uptake of exogenous folate by worms. A knockout mutation of the gene, folt-1(ok1460), was shown to cause sterility, and here we investigate the sourc...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Austin MU,Liau WS,Balamurugan K,Ashokkumar B,Said HM,LaMunyon CW

    更新日期:2010-05-04 00:00:00

  • Prolonged FGF signaling is necessary for lung and liver induction in Xenopus.

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    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Shifley ET,Kenny AP,Rankin SA,Zorn AM

    更新日期:2012-09-18 00:00:00

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    abstract:BACKGROUND:Super-resolution fluorescence microscopy performed via 3D structured illumination microscopy (3D-SIM) is well established on flat, adherent cells. However, blastomeres of mammalian embryos are non-adherent, round and large. Scanning whole mount mammalian embryos with 3D-SIM is prone to failure due to the mov...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Popken J,Dahlhoff M,Guengoer T,Wolf E,Zakhartchenko V

    更新日期:2015-11-26 00:00:00

  • In vitro culture of embryonic mouse intestinal epithelium: cell differentiation and introduction of reporter genes.

    abstract:BACKGROUND:Study of the normal development of the intestinal epithelium has been hampered by a lack of suitable model systems, in particular ones that enable the introduction of exogenous genes. Production of such a system would advance our understanding of normal epithelial development and help to shed light on the pa...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Quinlan JM,Yu WY,Hornsey MA,Tosh D,Slack JM

    更新日期:2006-05-25 00:00:00

  • Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing.

    abstract:BACKGROUND:The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Dussmann P,Pagel JI,Vogel S,Magnusson T,Zimmermann R,Wagner E,Schaper W,Ogris M,Deindl E

    更新日期:2011-05-20 00:00:00