Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development.


BACKGROUND:The FERM domain containing protein Mosaic Eyes (Moe) interacts with Crumbs proteins, which are important regulators of apical identity and size. In zebrafish, loss-of-function mutations in moe result in defects in brain ventricle formation, retinal pigmented epithelium and neural retinal development, pericardial edema, and tail curvature. In humans and mice, there are two major alternately spliced isoforms of the Moe orthologue, Erythrocyte Protein Band 4.1-Like 5 (Epb4.1l5), which we have named Epb4.1l5long and Epb4.1l5short, that differ after the FERM domain. Interestingly, Moe and both Epb4.1l5 isoforms have a putative C' terminal Type-I PDZ-Binding Domain (PBD). We previously showed that the N' terminal FERM domain in Moe directly mediates interactions with Crumbs proteins and Nagie oko (Nok) in zebrafish, but the function of the C'terminal half of Moe/Epb4.1l5 has not yet been examined. RESULTS:To define functionally important domains in zebrafish Moe and murine Epb4.1l5, we tested whether injection of mRNAs encoding these proteins could rescue defects in zebrafish moe- embryos. Injection of either moe or epb4.1l5long mRNA, but not epb4.1l5short mRNA, could rescue moe- embryonic defects. We also tested whether mRNA encoding C' terminal truncations of Epb4.1l5long or chimeric constructs with reciprocal swaps of the isoform-specific PBDs could rescue moe- defects. We found that injection of the Epb4.1l5short chimera (Epb4.1l5short+long_PBD), containing the PBD from Epb4.1l5long, could rescue retinal and RPE defects in moe- mutants, but not brain ventricle formation. Injection of the Epb4.1l5long chimera (Epb4.1l5long+short_PBD), containing the PBD from Epb4.1l5short, rescued retinal defects, and to a large extent rescued RPE integrity. The only construct that caused a dominant phenotype in wild-type embryos, was Epb4.1l5long+short_PBD, which caused brain ventricle defects and edema that were similar to those observed in moe- mutants. Lastly, the morphology of rod photoreceptors in moe- mutants where embryonic defects were rescued by moe or epb4.1l5long mRNA injection is abnormal and their outer segments are larger than normal. CONCLUSION:Taken together, the data reveal tissue specificity for the function of the PBD in Epb4.1l5long, and suggest that additional C' terminal sequences are important for zebrafish retinal development. Additionally, our data provide further evidence that Moe is a negative regulator of rod outer segment size.


BMC Dev Biol


Christensen AK,Jensen AM




Has Abstract


2008-01-11 00:00:00










  • Cell dedifferentiation and epithelial to mesenchymal transitions during intestinal regeneration in H. glaberrima.

    abstract:BACKGROUND:Determining the type and source of cells involved in regenerative processes has been one of the most important goals of researchers in the field of regeneration biology. We have previously used several cellular markers to characterize the cells involved in the regeneration of the intestine in the sea cucumbe...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: García-Arrarás JE,Valentín-Tirado G,Flores JE,Rosa RJ,Rivera-Cruz A,San Miguel-Ruiz JE,Tossas K

    更新日期:2011-10-17 00:00:00

  • Diapause in the pea aphid (Acyrthosiphon pisum) is a slowing but not a cessation of development.

    abstract:BACKGROUND:Many insects undergo a period of arrested development, called diapause, to avoid seasonally recurring adverse conditions. Whilst the phenology and endocrinology of insect diapause have been well studied, there has been comparatively little research into the developmental details of diapause. We investigated ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Shingleton AW,Sisk GC,Stern DL

    更新日期:2003-08-08 00:00:00

  • A comparative study of prenatal development in Miniopterus schreibersii fuliginosus, Hipposideros armiger and H. pratti.

    abstract:BACKGROUND:Bats comprise the second largest order of mammals. However, there are far fewer morphological studies of post-implantation embryonic development than early embryonic development in bats. RESULTS:We studied three species of bats (Miniopterus schreibersii fuliginosus, Hipposideros armiger and H. pratti), repr...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Wang Z,Han N,Racey PA,Ru B,He G

    更新日期:2010-01-21 00:00:00

  • Interleukin-6 increases inner cell mass numbers in bovine embryos.

    abstract:BACKGROUND:Work in other species suggests that interleukin-6 (IL6) promotes early embryo development. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production. RESULTS:Transcripts for IL6 and its two co...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Wooldridge LK,Ealy AD

    更新日期:2019-02-01 00:00:00

  • Histone deacetylase expression patterns in developing murine optic nerve.

    abstract:BACKGROUND:Histone deacetylases (HDACs) play important roles in glial cell development and in disease states within multiple regions of the central nervous system. However, little is known about HDAC expression or function within the optic nerve. As a first step in understanding the role of HDACs in optic nerve, this s...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Tiwari S,Dharmarajan S,Shivanna M,Otteson DC,Belecky-Adams TL

    更新日期:2014-07-09 00:00:00

  • Construction of a cDNA library for miniature pig mandibular deciduous molars.

    abstract:BACKGROUND:The miniature pig provides an excellent experimental model for tooth morphogenesis because its diphyodont and heterodont dentition resembles that of humans. However, little information is available on the process of tooth development or the exact molecular mechanisms controlling tooth development in miniatur...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Song T,Wu T,Wei F,Li A,Wang F,Xie Y,Liu D,Fan Z,Wang X,Cheng S,Zhang C,He J,Wang S

    更新日期:2014-04-21 00:00:00

  • Amnion formation in the mouse embryo: the single amniochorionic fold model.

    abstract:BACKGROUND:Despite the detailed knowledge obtained over the last decade on the molecular regulation of gastrulation in amniotes, the process of amnion development has been poorly described and illustrated in mice, and conflicting descriptions exist. Understanding the morphogenesis and development not only of the early ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Pereira PN,Dobreva MP,Graham L,Huylebroeck D,Lawson KA,Zwijsen AN

    更新日期:2011-08-01 00:00:00

  • The chick somitogenesis oscillator is arrested before all paraxial mesoderm is segmented into somites.

    abstract:BACKGROUND:Somitogenesis is the earliest sign of segmentation in the developing vertebrate embryo. This process starts very early, soon after gastrulation has initiated and proceeds in an anterior-to-posterior direction during body axis elongation. It is widely accepted that somitogenesis is controlled by a molecular o...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Tenin G,Wright D,Ferjentsik Z,Bone R,McGrew MJ,Maroto M

    更新日期:2010-02-25 00:00:00

  • myGRN: a database and visualisation system for the storage and analysis of developmental genetic regulatory networks.

    abstract:BACKGROUND:Biological processes are regulated by complex interactions between transcription factors and signalling molecules, collectively described as Genetic Regulatory Networks (GRNs). The characterisation of these networks to reveal regulatory mechanisms is a long-term goal of many laboratories. However compiling, ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Bacha J,Brodie JS,Loose MW

    更新日期:2009-06-06 00:00:00

  • Apical accumulation of MARCKS in neural plate cells during neurulation in the chick embryo.

    abstract:BACKGROUND:The neural tube is formed by morphogenetic movements largely dependent on cytoskeletal dynamics. Actin and many of its associated proteins have been proposed as important mediators of neurulation. For instance, mice deficient in MARCKS, an actin cross-linking membrane-associated protein that is regulated by ...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Zolessi FR,Arruti C

    更新日期:2001-01-01 00:00:00

  • Multiple enhancers contribute to spatial but not temporal complexity in the expression of the proneural gene, amos.

    abstract:BACKGROUND:The regulation of proneural gene expression is an important aspect of neurogenesis. In the study of the Drosophila proneural genes, scute and atonal, several themes have emerged that contribute to our understanding of the mechanism of neurogenesis. First, spatial complexity in proneural expression results fr...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Holohan EE,zur Lage PI,Jarman AP

    更新日期:2006-11-09 00:00:00

  • 3D structured illumination microscopy of mammalian embryos and spermatozoa.

    abstract:BACKGROUND:Super-resolution fluorescence microscopy performed via 3D structured illumination microscopy (3D-SIM) is well established on flat, adherent cells. However, blastomeres of mammalian embryos are non-adherent, round and large. Scanning whole mount mammalian embryos with 3D-SIM is prone to failure due to the mov...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Popken J,Dahlhoff M,Guengoer T,Wolf E,Zakhartchenko V

    更新日期:2015-11-26 00:00:00

  • Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells.

    abstract:BACKGROUND:Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs have not been completely unders...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Wang Z,Huang Y,Tan L

    更新日期:2020-01-14 00:00:00

  • Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain.

    abstract:BACKGROUND:The transcription factor Pax6 is essential for the development of the central nervous system and it exerts its multiple functions by regulating the expression of downstream target molecules. To screen for genes downstream of Pax6, we performed comprehensive transcriptome profiling analyses in the early hindb...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Numayama-Tsuruta K,Arai Y,Takahashi M,Sasaki-Hoshino M,Funatsu N,Nakamura S,Osumi N

    更新日期:2010-01-18 00:00:00

  • Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L.

    abstract:BACKGROUND:Formation of haploid spermatozoa capable of fertilization requires proper programming of epigenetic information. Exactly how DNMT3L (DNA methyltransferase 3-Like), a postulated regulator of DNA methyltransferase activity, contributes to DNA methylation pattern acquisition during gametogenesis remains unclear...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: La Salle S,Oakes CC,Neaga OR,Bourc'his D,Bestor TH,Trasler JM

    更新日期:2007-09-18 00:00:00

  • Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary.

    abstract:BACKGROUND:During Drosophila oogenesis, the follicular epithelium differentiates into several morphologically distinct follicle-cell populations. Characteristic bioelectrical properties make this tissue a suitable model system for studying connections between electrochemical signals and the organisation of the cytoskel...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Weiß I,Bohrmann J

    更新日期:2019-11-12 00:00:00

  • Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.

    abstract:BACKGROUND:Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously reported human pancreas organoid (hPO) cultures do not expand efficiently long-term in a chemic...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Georgakopoulos N,Prior N,Angres B,Mastrogiovanni G,Cagan A,Harrison D,Hindley CJ,Arnes-Benito R,Liau SS,Curd A,Ivory N,Simons BD,Martincorena I,Wurst H,Saeb-Parsy K,Huch M

    更新日期:2020-02-26 00:00:00

  • Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation.

    abstract:BACKGROUND:Tissue fixation is crucial for preserving the morphology of biological structures and cytological details to prevent postmortem degradation and autolysis. Improper fixation conditions could lead to artifacts and thus incorrect conclusions in immunofluorescence or histology experiments. To resolve reported st...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Nie J,Mahato S,Zelhof AC

    更新日期:2015-02-03 00:00:00

  • Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

    abstract:BACKGROUND:Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish. RESULTS:...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Vogeler S,Carboni S,Li X,Nevejan N,Monaghan SJ,Ireland JH,Joyce A

    更新日期:2020-11-23 00:00:00

  • β-catenin is required in the neural crest and mesencephalon for pituitary gland organogenesis.

    abstract:BACKGROUND:The pituitary gland is a highly vascularized tissue that requires coordinated interactions between the neural ectoderm, oral ectoderm, and head mesenchyme during development for proper physiological function. The interactions between the neural ectoderm and oral ectoderm, especially the role of the pituitary...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Davis SW,Mortensen AH,Keisler JL,Zacharias AL,Gage PJ,Yamamura K,Camper SA

    更新日期:2016-05-16 00:00:00

  • The Lc3-synthase gene B3gnt5 is essential to pre-implantation development of the murine embryo.

    abstract:BACKGROUND:Glycosphingolipids (GSL) are integral components of mammalian cell membranes that are involved in cell adhesion and cell signaling processes. GSL are subdivided into structural series, like ganglio-, lacto/neolacto-, globo- and isoglo-series, which are defined by distinct trisaccharide cores. The beta1,3 N-a...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Biellmann F,Hülsmeier AJ,Zhou D,Cinelli P,Hennet T

    更新日期:2008-11-12 00:00:00

  • Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick.

    abstract:BACKGROUND:MiR-9 is a small non-coding RNA that is highly conserved between species and primarily expressed in the central nervous system (CNS). It is known to influence proliferation and neuronal differentiation in the brain and spinal cord of different vertebrates. Different studies have pointed to regional and speci...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Alwin Prem Anand A,Huber C,Asnet Mary J,Gallus N,Leucht C,Klafke R,Hirt B,Wizenmann A

    更新日期:2018-02-22 00:00:00

  • Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi.

    abstract:BACKGROUND:Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene silencing in late clea...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Soares ML,Haraguchi S,Torres-Padilla ME,Kalmar T,Carpenter L,Bell G,Morrison A,Ring CJ,Clarke NJ,Glover DM,Zernicka-Goetz M

    更新日期:2005-12-28 00:00:00

  • Role of the 2 zebrafish survivin genes in vasculo-angiogenesis, neurogenesis, cardiogenesis and hematopoiesis.

    abstract:BACKGROUND:Normal growth and development of organisms requires maintenance of a dynamic balance between systems that promote cell survival and those that induce apoptosis. The molecular mechanisms that regulate these processes remain poorly understood, and thus further in vivo study is required. Survivin is a member of...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Delvaeye M,De Vriese A,Zwerts F,Betz I,Moons M,Autiero M,Conway EM

    更新日期:2009-03-26 00:00:00

  • Activated Ras alters lens and corneal development through induction of distinct downstream targets.

    abstract:BACKGROUND:Mammalian Ras genes regulate diverse cellular processes including proliferation and differentiation and are frequently mutated in human cancers. Tumor development in response to Ras activation varies between different tissues and the molecular basis for these variations are poorly understood. The murine lens...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Burgess D,Zhang Y,Siefker E,Vaca R,Kuracha MR,Reneker L,Overbeek PA,Govindarajan V

    更新日期:2010-01-27 00:00:00

  • Characterization of the astacin family of metalloproteases in C. elegans.

    abstract:BACKGROUND:Astacins are a large family of zinc metalloproteases found in bacteria and animals. They have diverse roles ranging from digestion of food to processing of extracellular matrix components. The C. elegans genome contains an unusually large number of astacins, of which the majority have not been functionally c...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Park JO,Pan J,Möhrlen F,Schupp MO,Johnsen R,Baillie DL,Zapf R,Moerman DG,Hutter H

    更新日期:2010-01-28 00:00:00

  • Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst.

    abstract:BACKGROUND:The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Ozawa M,Sakatani M,Yao J,Shanker S,Yu F,Yamashita R,Wakabayashi S,Nakai K,Dobbs KB,Sudano MJ,Farmerie WG,Hansen PJ

    更新日期:2012-11-06 00:00:00

  • Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques.

    abstract:BACKGROUND:Retinal cell development has been extensively investigated; however, the current knowledge of dynamic morphological and molecular changes is not yet complete. RESULTS:This study was aimed at revealing the dynamic morphological and molecular changes in retinal cell development during the embryonic stages usi...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Doh ST,Hao H,Loh SC,Patel T,Tawil HY,Chen DK,Pashkova A,Shen A,Wang H,Cai L

    更新日期:2010-01-20 00:00:00

  • Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos.

    abstract:BACKGROUND:Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo development and to optimize assisted reproductive technologies. Critical to the successful application of real-time PCR is carefu...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Goossens K,Van Poucke M,Van Soom A,Vandesompele J,Van Zeveren A,Peelman LJ

    更新日期:2005-12-03 00:00:00

  • Distinct contextual roles for Notch signalling in skeletal muscle stem cells.

    abstract::Notch signalling acts in virtually every tissue during the lifetime of metazoans. Recent studies have pointed to multiple roles for Notch in stem cells during quiescence, proliferation, temporal specification, and maintenance of the niche architecture. Skeletal muscle has served as an excellent paradigm to examine the...

    journal_title:BMC developmental biology

    pub_type: 杂志文章


    authors: Mourikis P,Tajbakhsh S

    更新日期:2014-01-24 00:00:00