Abstract:
BACKGROUND:The FERM domain containing protein Mosaic Eyes (Moe) interacts with Crumbs proteins, which are important regulators of apical identity and size. In zebrafish, loss-of-function mutations in moe result in defects in brain ventricle formation, retinal pigmented epithelium and neural retinal development, pericardial edema, and tail curvature. In humans and mice, there are two major alternately spliced isoforms of the Moe orthologue, Erythrocyte Protein Band 4.1-Like 5 (Epb4.1l5), which we have named Epb4.1l5long and Epb4.1l5short, that differ after the FERM domain. Interestingly, Moe and both Epb4.1l5 isoforms have a putative C' terminal Type-I PDZ-Binding Domain (PBD). We previously showed that the N' terminal FERM domain in Moe directly mediates interactions with Crumbs proteins and Nagie oko (Nok) in zebrafish, but the function of the C'terminal half of Moe/Epb4.1l5 has not yet been examined. RESULTS:To define functionally important domains in zebrafish Moe and murine Epb4.1l5, we tested whether injection of mRNAs encoding these proteins could rescue defects in zebrafish moe- embryos. Injection of either moe or epb4.1l5long mRNA, but not epb4.1l5short mRNA, could rescue moe- embryonic defects. We also tested whether mRNA encoding C' terminal truncations of Epb4.1l5long or chimeric constructs with reciprocal swaps of the isoform-specific PBDs could rescue moe- defects. We found that injection of the Epb4.1l5short chimera (Epb4.1l5short+long_PBD), containing the PBD from Epb4.1l5long, could rescue retinal and RPE defects in moe- mutants, but not brain ventricle formation. Injection of the Epb4.1l5long chimera (Epb4.1l5long+short_PBD), containing the PBD from Epb4.1l5short, rescued retinal defects, and to a large extent rescued RPE integrity. The only construct that caused a dominant phenotype in wild-type embryos, was Epb4.1l5long+short_PBD, which caused brain ventricle defects and edema that were similar to those observed in moe- mutants. Lastly, the morphology of rod photoreceptors in moe- mutants where embryonic defects were rescued by moe or epb4.1l5long mRNA injection is abnormal and their outer segments are larger than normal. CONCLUSION:Taken together, the data reveal tissue specificity for the function of the PBD in Epb4.1l5long, and suggest that additional C' terminal sequences are important for zebrafish retinal development. Additionally, our data provide further evidence that Moe is a negative regulator of rod outer segment size.
journal_name
BMC Dev Bioljournal_title
BMC developmental biologyauthors
Christensen AK,Jensen AMdoi
10.1186/1471-213X-8-3subject
Has Abstractpub_date
2008-01-11 00:00:00pages
3issn
1471-213Xpii
1471-213X-8-3journal_volume
8pub_type
杂志文章abstract:BACKGROUND:Fertilization in Caenorhabditis elegans requires functional SPE-9 protein in sperm. SPE-9 is a transmembrane protein with a predicted extracellular domain that contains ten epidermal growth factor (EGF)-like motifs. The presence of these EGF-like motifs suggests that SPE-9 is likely to function in gamete adh...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-3-10
更新日期:2003-12-03 00:00:00
abstract:BACKGROUND:The developing eye receives blood supply from two vascular systems, the intraocular hyaloid system and the superficial choroidal vessels. In zebrafish, a highly stereotypic and simple set of vessels develops on the surface of the eye prior to development of choroidal vessels. The origins and formation of thi...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/s12861-015-0066-9
更新日期:2015-03-27 00:00:00
abstract:BACKGROUND:Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS:We describe here involvement of the signaling enzyme Phosp...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-6-60
更新日期:2006-12-07 00:00:00
abstract:BACKGROUND:Circadian clocks have been implicated in the regulation of pre-adult development of fruit flies Drosophila melanogaster. It is believed that faster clocks speed up development and slower clocks slow it down. We established three sets of D. melanogaster populations (early, control and late). The early and lat...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-6-57
更新日期:2006-11-28 00:00:00
abstract:BACKGROUND:Formation of branching tubes is a fundamental step in the development of glandular organs. To identify extracellular cues that orchestrate epithelial tubulogenesis, we employed an in vitro assay in which EpH4-J3B1A mammary epithelial cells form spheroidal cysts when grown in collagen gels under serum-free co...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-7-7
更新日期:2007-02-08 00:00:00
abstract:BACKGROUND:Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on L...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/s12861-020-00225-1
更新日期:2020-10-06 00:00:00
abstract:BACKGROUND:The regulation of proneural gene expression is an important aspect of neurogenesis. In the study of the Drosophila proneural genes, scute and atonal, several themes have emerged that contribute to our understanding of the mechanism of neurogenesis. First, spatial complexity in proneural expression results fr...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-6-53
更新日期:2006-11-09 00:00:00
abstract:BACKGROUND:Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo development and to optimize assisted reproductive technologies. Critical to the successful application of real-time PCR is carefu...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-5-27
更新日期:2005-12-03 00:00:00
abstract:BACKGROUND:Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene silencing in late clea...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-5-28
更新日期:2005-12-28 00:00:00
abstract:BACKGROUND:The central nervous tissue contains diverse subtypes of neurons with characteristic morphological and physiological features and different neurotransmitter phenotypes. The generation of neurons with defined neurotransmitter phenotypes seems to be governed by factors differently expressed along the anterior-p...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-8-89
更新日期:2008-09-22 00:00:00
abstract:BACKGROUND:Vertebrate neural development requires precise coordination of cell proliferation and cell specification to guide orderly transition of mitotically active precursor cells into different types of post-mitotic neurons and glia. Lateral inhibition, mediated by the Delta-Notch signaling pathway, may provide a me...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213x-1-13
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Many insects undergo a period of arrested development, called diapause, to avoid seasonally recurring adverse conditions. Whilst the phenology and endocrinology of insect diapause have been well studied, there has been comparatively little research into the developmental details of diapause. We investigated ...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-3-7
更新日期:2003-08-08 00:00:00
abstract:BACKGROUND:Mammalian Ras genes regulate diverse cellular processes including proliferation and differentiation and are frequently mutated in human cancers. Tumor development in response to Ras activation varies between different tissues and the molecular basis for these variations are poorly understood. The murine lens...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-10-13
更新日期:2010-01-27 00:00:00
abstract:BACKGROUND:The miniature pig provides an excellent experimental model for tooth morphogenesis because its diphyodont and heterodont dentition resembles that of humans. However, little information is available on the process of tooth development or the exact molecular mechanisms controlling tooth development in miniatur...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-14-16
更新日期:2014-04-21 00:00:00
abstract:BACKGROUND:Neuroblastoma is a childhood cancer derived from immature cells of the sympathetic nervous system. The disease is clinically heterogeneous, ranging from neuronal differentiated benign ganglioneuromas to aggressive metastatic tumours with poor prognosis. Amplification of the MYCN oncogene is a well establishe...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-11-1
更新日期:2011-01-03 00:00:00
abstract:BACKGROUND:Postnatal growth in mouse is rapid, with total skeletal muscle mass increasing several-fold in the first few weeks. Muscle growth can be achieved by either an increase in muscle fibre number or an increase in the size of individual myofibres, or a combination of both. Where myofibre hypertrophy during growth...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-10-21
更新日期:2010-02-22 00:00:00
abstract:BACKGROUND:The neural tube is formed by morphogenetic movements largely dependent on cytoskeletal dynamics. Actin and many of its associated proteins have been proposed as important mediators of neurulation. For instance, mice deficient in MARCKS, an actin cross-linking membrane-associated protein that is regulated by ...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213x-1-7
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Interactions of cells with the extracellular matrix (ECM) are critical for the establishment and maintenance of stem cell self-renewal and differentiation. However, the ECM is a complex mixture of matrix molecules; little is known about the role of ECM components in human embryonic stem cell (hESC) different...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-8-90
更新日期:2008-09-22 00:00:00
abstract:BACKGROUND:Determining the type and source of cells involved in regenerative processes has been one of the most important goals of researchers in the field of regeneration biology. We have previously used several cellular markers to characterize the cells involved in the regeneration of the intestine in the sea cucumbe...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-11-61
更新日期:2011-10-17 00:00:00
abstract:BACKGROUND:During early steps of embryonic development the hindbrain undergoes a regionalization process along the anterior-posterior (AP) axis that leads to a metameric organization in a series of rhombomeres (r). Refinement of the AP identities within the hindbrain requires the establishment of local signaling center...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-9-61
更新日期:2009-12-03 00:00:00
abstract:BACKGROUND:One way in which positional information is established during embryonic development is through the graded distribution of diffusible morphogens. Unfortunately, little is known about how cells interpret different concentrations of morphogen to activate different genes or how thresholds are generated in a morp...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-7-47
更新日期:2007-05-16 00:00:00
abstract:BACKGROUND:Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs have not been completely unders...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/s12861-019-0206-8
更新日期:2020-01-14 00:00:00
abstract:BACKGROUND:Heparan sulfate (HS) biosynthesis is tightly regulated during vertebrate embryo development. However, potential roles for HS biosynthesis in regulating the function of paracrine signaling molecules that bind to HS are incompletely understood. RESULTS:In this report we have studied Fgf, Wnt and Hedgehog (Hh)...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-11-53
更新日期:2011-09-05 00:00:00
abstract:BACKGROUND:Normal post-squalene cholesterol biosynthesis is important for mammalian embryonic development. Neonatal mice lacking functional dehydrocholesterol Delta7-reductase (Dhcr7), a model for the human disease of Smith-Lemli-Opitz syndrome, die within 24 hours of birth. Although they have a number of biochemical a...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-4-1
更新日期:2004-02-02 00:00:00
abstract:BACKGROUND:Pooled human embryonic stem cells (hESC) cell lines were profiled to obtain a comprehensive list of genes common to undifferentiated human embryonic stem cells. RESULTS:Pooled hESC lines were profiled to obtain a comprehensive list of genes common to human ES cells. Massively parallel signature sequencing (...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-4-10
更新日期:2004-08-10 00:00:00
abstract:BACKGROUND:Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the bl...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/s12861-019-0195-7
更新日期:2019-07-05 00:00:00
abstract:BACKGROUND:Mitogen-activated protein kinase (MAPK) cascades (p38, JNK, ERK pathways) are involved in cell fate acquisition during development. These kinase modules are associated with scaffold proteins that control their activity. In Drosophila, dMP1, that encodes an ERK scaffold protein, regulates ERK signaling during...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-11-17
更新日期:2011-03-14 00:00:00
abstract:BACKGROUND:The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-12-33
更新日期:2012-11-06 00:00:00
abstract:BACKGROUND:The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. RESULTS:We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'kno...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-6-2
更新日期:2006-01-22 00:00:00
abstract:BACKGROUND:ERK5 is a member of the mitogen activated protein kinase family activated by certain mitogenic or stressful stimuli in cells, but whose physiological role is largely unclear. RESULTS:To help determine the function of ERK5 we have used gene targeting to inactivate this gene in mice. Here we report that ERK5 ...
journal_title:BMC developmental biology
pub_type: 杂志文章
doi:10.1186/1471-213X-3-11
更新日期:2003-12-16 00:00:00