Abstract:
OBJECTIVES:Advanced glycation end products (AGEs) have been implicated in the pathogenesis of bone-destructive disorders. Yet reports on the influence of AGEs on human osteoblasts remain lacking. The aim of the study is to investigate the influence of AGE-modified bovine serum albumin (AGE-BSA) on cell growth and expression of osteoblastic markers associated with osteogenesis and osteoclastogenesis. METHODS:Human osteoblasts established from bone tissue specimens were stimulated with AGE-BSA and investigated in vitro. Expression of mRNA for the receptor for AGEs (RAGE), nuclear factor kappa B subunit p65 (NFκB p65), tumour necrosis factor alpha (TNF-α), matrix metallo proteinase-1 (MMP-1), receptor activator of NFκB ligand (RANKL), osteoprotegerin, collagen type I (Col1), osteocalcin (OC) and alkaline phosphatase (ALP) were measured using real-time polymerase chain reaction (PCR). Respective protein expressions were evaluated by western blot analysis or ELISA. NFκB activation was investigated by luciferase assay and electrophoretic mobility shift assay (EMSA). Cell cycle analysis, cell proliferation and markers of necrosis and early apoptosis were assessed. RESULTS:AGE-BSA was actively taken up into osteoblasts and induced cell cycle arrest and an increase in necrotic, but not apoptotic cells. The increased expression of RAGE and TNF-α together with NFκB activation indicates an AGE-mediated inflammatory response. The decreased expression of Col1, OC and ALP presumably reflects a diminished osteogenic potential, whereas upregulation of RANKL and TNF-α enhances osteoclastogenesis. CONCLUSIONS:The present study demonstrates that AGE-BSA affects the growth and function of osteoblasts. Modulation of the expression of various target genes involved in bone metabolism provides evidence that AGEs accumulated in the bone matrix have the potential to suppress osteogenic and to promote osteoclastogenic properties of osteoblasts in vivo, thereby leading to functional and structural impairment of bone.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Franke S,Rüster C,Pester J,Hofmann G,Oelzner P,Wolf Gsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
650-60issue
4eissn
0392-856Xissn
1593-098Xpii
4219journal_volume
29pub_type
杂志文章abstract:OBJECTIVES:Antibodies directed to citrullinated proteins are highly specific for rheumatoid arthritis (RA). Citrullination is catalyzed by peptidylarginine deiminase (PAD) enzymes. The current study examined the mRNA expression of PADI2 and PADI4 in bone marrow (BM) CD34+ cells from RA patients. METHODS:CD34+ cells we...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2018-03-01 00:00:00
abstract::Fibromyalgia syndrome (FMS) is a common form of non-inflammatory rheumatism within the general population with symptoms often mimicking those of arthritis or muscle disorders. Arthralgic symptoms in the region of the hip are commonly mentioned by patients with FMS and one of the diagnostic trigger points for the condi...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,多中心研究
doi:
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVES:This study investigated whether: (i) rheumatoid arthritis (RA) patients have more micronuclei (MN) than healthy controls; (ii) methotrexate (MTX) treated RA patients have more MN than those not using MTX, and (iii) folic acid supplementation decreases the number of MN in MTX treated patients. METHODS:MN ass...
journal_title:Clinical and experimental rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-03-01 00:00:00
abstract::A 61-year-old woman, suffering from classic seropositive rheumatoid arthritis with rheumatoid nodule histologically documented, developed temporal arteritis. HLA-DR typing revealed the presence of DR3 and DR4 antigens. The findings from previous studies support the association of HLA-DR antigens, giant cell arteritis-...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
abstract:OBJECTIVES:Giant cell arteritis (GCA) is a cause of potentially fatal aortic aneurysms. Descriptive data on thoracic aorta measurements at the beginning of the disease are lacking. We aimed to compare aortic diameters between a recently diagnosed GCA population and an age- and sex-matched control group. METHODS:Patien...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:The primary objective of this study was to evaluate the ex vivo therapeutic efficacy of diacerein and its active metabolite, rhein, on osteoarthritic (OA) cartilage chondrocyte DNA fragmentation and death in the experimental canine model of OA. The study also aimed to explore the effect of the drug on the lev...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:OBJECTIVES:An educational programme was conducted in Italy in order to favour the diffusion of the rheumatoid arthritis (RA) treat-to-target (T2T) recommendations among Italian rheumatologists. Our objective was to measure the level of acceptance and applicability of the 10 recommendations to treat RA to a target of re...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Herewith we provide a critical digest of the recent literature on systemic sclerosis. The most relevant studies published over the last two years have been reviewed, with particular focus on the diagnosis, pathogenesis and treatment of the disease. ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG), prostaglandin E2 (PGE2) and cytokines (IL-6 and IL-8) by human articular chondrocytes in vitro. METHODS:Enzymatically isolated...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVES:To identify the role of nailfold capillaroscopy (NC) in Sjögren's syndrome (SS). METHODS:The literature was systematically reviewed in three databases. All published original studies which assess patients with SS by NC were revised. A quality assessment was applied to all studies based on population descrip...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Single nucleotide polymorphisms of CD11a and CD11c have been suggested as susceptibility loci in Korean patients with Behçet's disease (BD). As immunoregulatory roles of CD11c+CD8+T cells were previously observed in multiple autoimmune and autoinflammatory diseases, we aimed to investigate CD11a and CD11c in...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:Examining for gastrointestinal involvement in juvenile idiopathic arthritis is an important part of diagnostic and therapeutic procedures. Only few scientific data are available. METHODS:In a prospective study, 41 patients with juvenile idiopathic arthritis were examined for clinical and laboratory data of g...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::To test whether alternative complement pathway activation explains normal C1s-C1 inhibitor complex in hypocomplementemic (low CH50cl) patients with systemic lupus erythematosus, we examined alternative pathway hemolytic complement (CH50alt) factor B, and Ba fragment in hypocomplementemic sera with normal and with elev...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is an autoimmune inflammatory disease associated with rapid loss of function and radiographic damage. Treatment is targeted to achieve low disease activity/remission, as measured by various pooled indices comprised of laboratory measures, patient-derived, and physician-derived measures. Outsi...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:Coeliac disease (CD) is a chronic autoimmune disease of the small intestine caused by the ingestion of gluten, in which musculoskeletal manifestations may occur. Aim of this study was to evaluate the prevalence and severity of joint involvement in paediatric patients with CD using musculoskeletal ultrasound ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Methotrexate (MTX) in low doses is widely used in the treatment of rheumatoid arthritis (RA) and it is not known whether its effects are due to immunosuppressive and/or anti-inflammatory actions. High concentrations of MTX inhibit the activity of thymidylate synthetase (TS) and dihydrofolate reductase essenti...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::The association of kerato-conjunctivitis sicca and xerostomia has been termed Sjogren's syndrome (SS). Although this disease is referred to as a non-organ-specific autoimmune condition, the vast majority of the deleterious effects of primary SS are restricted to the exocrine glands. Among them, the lacrymal and saliva...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2004-09-01 00:00:00
abstract::We report the emergence of polyclonal expansion of gut lymphocyte associated tissues producing IgA in a patient with rheumatoid arthritis associated with a high serum IgA concentration. The role of IgA in rheumatoid arthritis is reviewed. ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::MALT, or mucosa associated lymphoid tissue, is normally not present in gastric tissue. Its presence is often associated with persistent antigenic stimulation. MALT is a precursor of gastric MALT lymphoma, a low-grade lymphoma whose incidence recently appears to have increased. Although much epidemiologic and clinical ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVES:To describe the MRI features of gout tophi in the soft-tissues or joints of the limbs by low-field extremity-dedicated MRI. METHODS:Nine consecutive patients, 8M/1W, affected by chronic tophaceous gout were studied. Mean patients' age was 71.3±11.5 years, mean disease duration 98.1±44.9 months, and mean ser...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVES:A decrease in high-density lipoprotein (HDL) cholesterol during inflammation is common in many rheumatologic diseases, including rheumatoid arthritis (RA). Apolipoprotein M (apoM) is an apolipoprotein predominantly associated with HDL cholesterol. Recently, apoM polymorphisms have been related with RA suscep...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract::Heat shock proteins (hsp) or "stress proteins" are a group of highly conserved proteins which are important in the day to day function of all cells. Early studies by others have indicated that immunity to the 65 kDa hsp of mycobacteria is important in the development of arthritis in the adjuvant arthritis model in rat...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:1990-07-01 00:00:00
abstract:OBJECTIVES:To assess adherence to published guidelines for the treatment of Behçet's syndrome (BS) in two geographic areas. METHODS:We extracted guideline statements from the 2008 EULAR recommendations. Adherence to these statements was evaluated retrospectively in both New York (USA) and Amsterdam (The Netherlands), ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,多中心研究
doi:
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVES:To determine the protein expression of TNFAIP3 in synovium and to show the capability of 6q23 intergenic SNPs, associated with rheumatoid arthritis (RA) susceptibility, to influence TNFAIP3 gene transcription. METHODS:Immunohistochemistry for TNFAIP3, NF-kB p65 and phosphorylated NF-kB p65 protein expressio...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract::****************************************************************************. ...
journal_title:Clinical and experimental rheumatology
pub_type: 信件
doi:
更新日期:2017-03-01 00:00:00
abstract::Leg ulcers are associated with antiphospholipid antibodies in patients with systemic lupus erythematosus. They may also occur in patients with primary antiphospholipid syndrome(PAPS). Histopathologic findings at the edges of the ulcers may give insight into their pathogenesis. In a patient with PAPS and a leg ulcer, a...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Tumour necrosis factor-receptor associated periodic syndrome (TRAPS) is a rare autosomal dominant autoinflammatory disorder characterised by recurrent episodes of long-lasting fever and inflammation in different regions of the body, as musculo-skeletal system, skin, gastrointestinal tube, serosal membranes and eye. In...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVES:The Behçet's Syndrome Activity Score (BSAS) is the first patient reported outcome measure developed to assess the global disease activity in patients with Behçet's syndrome (BS). We aimed to evaluate the reliability and validity of the Turkish version of BSAS for measuring disease activity in BS. We further ...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVES:The CD40L/CD40 pathway is involved in the pathophysiology of atherothrombotic disease, and elevated levels of soluble CD40L (sCD40L) were reported in SLE patients. However, the clinical implication of sCD40L in SLE remains elusive. METHODS:We measured levels of plasma sCD40L in 241 SLE patients and 37 healt...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2017-09-01 00:00:00
abstract::Spondyloarthritides (SpA) are chronic inflammatory rheumatic diseases that usually affect the axial skeleton and may involve entheses and peripheral joints. The main subtypes are ankylosing spondylitis (AS) and psoriatic arthritis (PsA). Other subtypes are reactive arthritis, arthritis associated with chronic inflamma...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2011-09-01 00:00:00