Abstract：
PURPOSE:To test the hypothesis that RNA editing is altered in pediatric patients with cyanotic congenital heart disease (CHD) and to determine whether A-to-I RNA editing is associated with the postoperative course following cardiac surgery. Cyanotic CHD is associated with a unique pathophysiology caused by chronic hypoxia. The perioperative course of cyanotic infants is partly dictated by the degree of expression of inflammatory and cardiac genes, some of which undergo A-to-I RNA editing. METHODS:RNA was extracted pre- and postoperatively from blood samples of cyanotic and acyanotic patients. Each sample was analyzed for A-to-I RNA editing using automatic DNA sequencing of an intronic segment of the MED13 gene. RNA expression levels of adenosine deaminase acting on RNA (ADAR) enzymes responsible for RNA editing were examined by quantitative reverse-transcriptase polymerase chain reaction. RESULTS:A-to-I RNA editing in MED13 was significantly higher among cyanotic patients (n = 19) than acyanotic ones (n = 18) both pre- and postoperatively, as manifested by average editing at seven highly edited sites (27.4 ± 8.5% versus 20.8 ± 10.2%; P = 0.038) and editing at specific sites, e.g., position 14 (20.2 ± 5.1% versus 14.5 ± 5.2%; P = 0.002). Cyanotic patients exhibited a more complicated postoperative course than acyanotic patients. ADAR2 RNA levels were significantly lower among cyanotic patients. CONCLUSIONS:Cyanotic children manifest significantly higher rates of A-to-I RNA editing than acyanotic children as well as a more complicated surgical course. Posttranscriptional RNA changes may affect cellular and metabolic pathways and influence the perioperative course following hypoxia.
journal_name
Intensive Care Medjournal_title
Intensive care medicineauthors
Borik S,Simon AJ,Nevo-Caspi Y,Mishali D,Amariglio N,Rechavi G,Paret Gdoi
10.1007/s00134-011-2296-zsubject
Has Abstractpub_date
2011-10-01 00:00:00pages
1664-71issue
10eissn
0342-4642issn
1432-1238journal_volume
37pub_type
杂志文章abstract：PURPOSE:To evaluate the diagnostic accuracy of ultrasonography of optic nerve sheath diameter (ONSD) for assessment of intracranial hypertension. METHODS:Systematic review without language restriction based on electronic databases, with manual review of literature and conference proceedings until July 2010. Studies we...
journal_title：Intensive care medicine
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00134-011-2224-2
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:Limited data exist on the correlation between higher flow rates of high-flow nasal cannula (HFNC) and its physiologic effects in patients with acute hypoxemic respiratory failure (AHRF). We assessed the effects of HFNC delivered at increasing flow rate on inspiratory effort, work of breathing, minute ventilatio...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-017-4890-1
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:Organophosphate toxicity is the leading cause of morbidity and death in poisoning by insecticides. The clinical symptoms of pesticide toxicity range from the classic cholinergic syndrome to flaccid paralysis and intractable seizures. The mainstays of therapy are atropine, oximes, benzodiazepines and supportiv...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-005-0051-z
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVE:The study was undertaken to determine if critically ill patients under mechanical ventilation could reactivate latent cytomegalovirus (CMV) in either lung or blood. DESIGN:Prospective study in critically ill patients was performed in a multidisciplinary intensive care unit in a university hospital. PATIENTS...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01709343
更新日期：1996-11-01 00:00:00
abstract：:A case of brainstem encephalitis complicated by torsade de pointes is described. The possible occurrence of ventricular arrhythmias may contribute to the mortality in this condition. We recommend the admission of patients with brainstem encephalitis to an intensive care unit, for a period of electrocardiographic monit...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00263564
更新日期：1987-01-01 00:00:00
abstract：PURPOSE:Ventricular-arterial (V-A) decoupling decreases myocardial efficiency and is exacerbated by tachycardia that increases static arterial elastance (Ea). We thus investigated the effects of heart rate (HR) reduction on Ea in septic shock patients using the beta-blocker esmolol. We hypothesized that esmolol improve...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-016-4351-2
更新日期：2016-10-01 00:00:00
abstract：:Recombinant activated factor VII (rFVIIa, 'NovoSeven') is indicated for the treatment of spontaneous and surgical bleeding in patients with haemophilia A or B with antibodies to factors VIII or IX (FVIII or FIX) worldwide, and in patients with acquired haemophilia in Europe. In vitro cell models have demonstrated that...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-002-1469-1
更新日期：2002-10-01 00:00:00
abstract：:Specific regulations regarding oversight of research in children vary from country to country, but most share common principles derived from major consensus documents. Whereas the permissibility of research on adults depends heavily upon the informed consent of the subject, the regulation of research in pediatrics is ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-007-0917-3
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis whether PMN-Elastase in bronchoalveolar lavage fluid (BALF) could reflect neutrophil activity in the lower respiratory tract. DESIGN:Prospectively, morphologic findings of acute and chronic inflammation in the transbronchial lung biopsy specimens were compared with the numbers of neutr...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01720908
更新日期：1994-05-01 00:00:00
abstract：OBJECTIVE:To evaluate various treatment strategies in critically ill patients with ischaemic acute renal failure, there is a need for reliable bedside measurements of total renal blood flow (RBF), glomerular filtration rate (GFR) and renal oxygen consumption without the need for urine collection. DESIGN:The continuous...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s00134-004-2380-8
更新日期：2004-09-01 00:00:00
abstract：:We examined the incidence, diagnosis and therapy of acute adrenal insufficiency, secondary to adrenal hemorrhage. This insufficiency resulted in temperature irregularities. hemodynamic instability, and a large volume resuscitation requirement post-operatively. The case illustrates that a high level of suspicion should...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01704704
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVE:To determine the frequency and the eventual clinical characteristics of nosocomial sinusitis with anaerobic bacteria isolation in patients in the intensive care unit (ICU). DESIGN:Retrospective study. SETTING:A 12-bed medical ICU in a teaching hospital. PATIENTS:30 adult patients with documented nosocomial...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340051013
更新日期：1999-10-01 00:00:00
abstract：OBJECTIVE:Evaluation of an open circuit multiple breath nitrogen washout (MBNW) technique for measurement of functional residual capacity (FRC) during partial ventilatory support using corrections for gas viscosity, sampling delay time, and re-inspired nitrogen. DESIGN:Measurements in a lung model with known reference...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1677-3
更新日期：2003-05-01 00:00:00
abstract：:Since the introduction of synchronized intermittent mandatory ventilation (SIMV) several advantages have been attributed to this ventilatory mode, one of them being a more homogeneous distribution of ventilation and perfusion than during controlled mechanical ventilation (CMV). Up to now no data are available to confi...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01704716
更新日期：1991-01-01 00:00:00
abstract：:Breathing pattern was studied non-invasively in 20 coronary artery bypass surgery patients before the operation and post-operatively after weaning from mechanical ventilation. Post-operatively minute ventilation (VE), breathing frequency (Fr) and mean inspiratory flow (VT/TI) increased (28%, 42%, 27%; p less than 0.01...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01716198
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:We propose that higher airway occlusion pressure (P0.1) responses to hypercapnic challenge (HC) indicate less severe injury. The study aim was to determine whether P0.1 responses to HC were associated with successful weaning after prolonged mechanical ventilation (PMV) in patients with brainstem lesions and t...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-008-1197-2
更新日期：2009-01-01 00:00:00
abstract：:In the past two years several guidelines for suggested components of emergency medical systems for children have been suggested, and for the first time, specific standards of pediatric trauma care have also been formulated. The American Academy of Pediatrics new Provisional Committee on Emergency Medicine has been cha...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00260888
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:Providing mechanical ventilation is challenging at supra-atmospheric pressure. The higher gas density increases resistance, reducing the flow delivered by the ventilator. A new hyperbaric ventilator (Siaretron IPER 1000) is said to compensate for these effects automatically. The aim of this bench test study was...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-012-2590-4
更新日期：2012-08-01 00:00:00
abstract：PURPOSE:Inflammatory markers may have a role in predicting severity of illness of intensive care unit (ICU) patients. The aim of this study is to determine whether low eosinophil count can predict 28-day mortality in medical ICU. METHODS:A prospective study over a 4-month period. To evaluate the prognosis information ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-011-2170-z
更新日期：2011-07-01 00:00:00
abstract：:As a result of the previous shortage of tools to assess objectively the overall physiological status of the respiratory system in infants and young children, it has been difficult to measure the degree of physiological disorder or the response to therapy in respiratory diseases such as BPD, the pediatric version of AR...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/BF01704742
更新日期：1995-09-01 00:00:00
abstract：OBJECTIVE:To assess the effects of the cyclooxygenase inhibitor diclofenac in a canine model of pulmonary occlusion and reperfusion of the left lower lobe (LLL). DESIGN:Twelve adult beagle dogs (13-17 kg) were randomly assigned to a control group (n = 6) and a diclofenac-treated group (n = 6). Animals in the treatment...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01700965
更新日期：1995-10-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between cytokines and the inflammatory responses in patients with out-of-hospital cardiac arrest, we examined the changes of cytokines as well as alterations in the markers of neutrophil activation, platelet and endothelial activation, and endothelial injury. DESIGN:Prospectiv...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s001340050907
更新日期：1999-06-01 00:00:00
abstract：:Dynamic vectorcardiography (VCG) is increasingly employed for ischaemia monitoring with the use of a computerized method for recording and on-line analysis by the calculation of trend parameters. To elucidate how well the derived electrocardiogram (dECG), calculated from the VCG, compares with the simultaneously regis...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s001340050455
更新日期：1997-10-01 00:00:00
abstract：: ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-018-5213-x
更新日期：2019-01-01 00:00:00
abstract：:Physicians are increasingly involved in how their critically ill patients die [72]. The more this happens, the more physicians will have to understand not only how their own backgrounds and biases influence their medical management, but also the cultural and religious backgrounds of the patient and surrogate [72, 73]....
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s001340000641
更新日期：2000-10-01 00:00:00
abstract：OBJECTIVE:Comparison of side effects (fever shivering and/or allergic reactions, collapse, symptomatic hypocalcaemia) of the different substitution fluids used during therapeutical plasmapheresis. DESIGN:Multicentric retrospective comparative study designed to explore the side effects due to the replacements fluids (a...
journal_title：Intensive care medicine
pub_type： 杂志文章,多中心研究
doi：10.1007/s001340050595
更新日期：1998-05-01 00:00:00
abstract：OBJECTIVE:To compare the effectiveness of 70-mg and 200-mg doses of intravenous erythromycin in improving gastric emptying in critically ill patients. DESIGN:Gastric emptying was measured on consecutive days; day 1 (pre-treatment), day 2 (post-treatment) after an intravenous infusion of either 70 or 200 mg erythromyci...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00134-005-2663-8
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVE:To describe Enterococcus faecalis colonisation and endocarditis in 5 intensive care patients after treatment with selective decontamination (SDD). SETTING:Intensive care unit (ICU) in a general hospital. PATIENTS:The patients were admitted to the ICU because of adult respiratory distress syndrome, polytraum...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01701478
更新日期：1995-03-01 00:00:00
abstract：:Changes in the inspiratory flow rate of mechanically ventilated patients can affect arterial oxygenation. Although the mechanism producing this alteration is not certain, one possible mechanism is a change in ventilation inhomogeneity. This study was performed to determine if the inspiratory flow setting would affect ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00254316
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:Although several advantages are attributed to tracheotomy in ICU patients requiring mechanical ventilation (MV), true benefits and the optimal timing of tracheotomy remain controversial. In this study, we compared early tracheotomy (ET) with prolonged intubation (PI) in severely ill patients requiring prolong...
journal_title：Intensive care medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00134-008-1195-4
更新日期：2008-10-01 00:00:00
