Abstract：
:Dp71 has an important role in the central nervous system. To better understand the function of Dp71 domains in neuronal differentiation, PC12 cells were stably transfected with a dystrophin mutant, Dp71Δ(78-79) , which lacks exons 78 and 79. Based on the percentage of cells bearing neurites and neurite length analyses, we found that cells stably expressing Dp71Δ(78-79) (PC12-C11) differentiate more efficiently than non-transfected cells. While wild-type cells reach their maximum differentiation 9-12 days after initiating the differentiation process, the PC12-C11 cells reach differentiation in 4-6 days. Protein expression analysis showed a down-regulation of Dp71a and an up-regulation of Dp71ab and/or Up71, β-dystroglycan and neuron-specific enolase in undifferentiated and in neural growth factor differentiated PC12-C11 cells. No change was observed in the expression of Grb2 and Up400. The subcellular localization of Dp71Δ(78-79) was in the cell periphery, and there was no change in localization during the differentiation process, which was also observed throughout the neurite extensions.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Aragón J,Romo-Yáñez J,Martínez-Herrera A,Ceja V,Rendon A,Montañez Cdoi
10.1111/j.1471-4159.2011.07347.xsubject
Has Abstractpub_date
2011-11-01 00:00:00pages
697-707issue
4eissn
0022-3042issn
1471-4159journal_volume
119pub_type
杂志文章abstract：:A continuous enzyme-linked fluorometric assay was used for determining the characteristics for glutamate exocytosis from guinea-pig cerebrocortical synaptosomes. Ca2+-dependent release can be induced not only by K+, but also by the Na+ channel activator veratridine and the Ca2+ ionophore ionomycin. K+-induced release ...
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journal_title：Journal of neurochemistry
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
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更新日期：2018-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.2000.0742146.x
更新日期：2000-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1989.tb02524.x
更新日期：1989-03-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：2007-04-01 00:00:00
abstract：:As the leading genetic cause for infantile death, Spinal Muscular Atrophy (SMA) has been extensively studied since its first description in the early 1890s. Though today much is known about the cause of the disease, a cure or effective treatment is not currently available. Recently the short chain fatty acid valproic ...
journal_title：Journal of neurochemistry
pub_type： 杂志文章,评审
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更新日期：2009-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2006-02-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.2005.03363.x
更新日期：2005-10-01 00:00:00
abstract：:Stem cell-based approaches provide hope as a potential therapy for neurodegenerative diseases and stroke. One of the major scientific hurdles for stem cell therapy is the poor survival rate of the newly formed or transplanted neural stem cells. In this study, we found that low-dose treatment with the Heat shock protei...
journal_title：Journal of neurochemistry
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更新日期：2011-05-01 00:00:00
