Abstract：
:The AmpFℓSTR(®) Identifiler(®) Direct PCR Amplification Kit is a new short tandem repeat multiplex assay optimized to allow the direct amplification of single-source blood and buccal samples on FTA(®) card without the need for sample purification and quantification. This multiplex assay has been validated according to the FBI/National Standards and SWGDAM guidelines. Validation results revealed that slight variations in primer concentration, master mix component concentration, and thermal cycling parameters did not affect the performance of the chemistry. The assay's sensitivity was demonstrated by amplifying known amounts of white blood cells spotted onto FTA(®) cards, and the assay's specificity was verified by establishing minimal cross-reactivity with nonhuman DNA. No effect on the age of the sample stored on the FTA(®) substrate was observed and full concordance was established in the population study. These findings of the validation study support the use of the Identifiler(®) Direct Kit for forensic standards and database samples genotyping.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Wang DY,Chang CW,Lagacé RE,Oldroyd NJ,Hennessy LKdoi
10.1111/j.1556-4029.2011.01757.xsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
835-45issue
4eissn
0022-1198issn
1556-4029journal_volume
56pub_type
杂志文章abstract：:Ethanol was determined by gas chromatography in a variety of tissues and body fluids secured at autopsy in 61 cases. The specimens tested included right and left heart blood, femoral blood, pericardial fluid, cerebrospinal fluid, vitreous humor, urine, stomach contents, and brain. Statistical analysis of the cases rev...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Little is known about to what extent the events surrounding death can be reliably reconstructed from blunt force trauma analysis alone. In this study, we reconstruct circumstances of death from a blind blunt force trauma analysis using a sample of 21 individuals of known death circumstances, obtained from two identifi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13128
更新日期：2016-11-01 00:00:00
abstract：:The authors describe three cases (two blacks and one Latin American) of sudden death caused by late complications of Kawasaki disease (mucocutaneous lymph node syndrome). At autopsy each heart contained multiple coronary artery aneurysms with luminal stenosis caused by intimal hyperplasia and thrombi. Although virtual...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The aims of this study were to enable geometric morphometric sex classification using tibial proximal and distal sexual dimorphism and to evaluate the secular trend of tibial shape/form from the early 20th century to the present day. The study samples consisted of 61 adult tibias from an early 20th-century Czech popul...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：10.1111/1556-4029.12641
更新日期：2015-01-01 00:00:00
abstract：:The Computer-Assisted Postmortem Identification (CAPMI) system was developed at the U.S. Army Institute of Dental Research to facilitate rapid identification of human remains. The increasing requirement for dental based identification of high-energy fatalities and decomposed remains has dovetailed with the development...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:Age at death assessment of adult skeletons is one of the most difficult problems in forensic and physical anthropology. Two fundamental sources of error are described: the complex variability in the process of skeletal aging and methodological bias. Taking into account these limits, we developed a new scoring system f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Spontaneous pneumothorax is present in 1 to 2% of healthy full-term newborn infants. Virtually all cases have resolution of the pneumothorax without sequelae and in fact without the condition being diagnosed. Two fatal cases are presented. Anyone doing medicolegal autopsies should be aware of this entity so that X-ray...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:Gait is one biological characteristic which has attracted strong research interest due to its potential use in human identification. Although almost two decades have passed since a forensic gait expert has testified to the identity of a perpetrator in court, the methods remain insufficiently robust, considering the re...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/1556-4029.14036
更新日期：2019-09-01 00:00:00
abstract：:Many studies have been published describing numerous techniques to determine the aging of individuals from dental tissues. However, few case reports demonstrate how such techniques can be employed by the forensic odontologist undertaking casework. Indeed, many of the techniques are highly complex and utilize equipment...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:This study evaluated the reliability of Widmark calculations, based on breath ethanol reading (BrACs), for estimating the amount of alcohol consumed. A standard ethanol dose (males 0.51 g/kg; females 0.43 g/kg) was given to 115 college seniors, and BrACs were measured for two hours. Calculations of ethanol dose were p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The above article, published online on January 27, 2020, in Wiley Online Library, has been withdrawn by agreement between the authors, the JFS Editor in Chief (Dr. Michael Peat), and John Wiley & Sons Ltd. The withdrawal has been agreed by all parties given the ongoing confidential legal proceeding concerning the deat...
journal_title：Journal of forensic sciences
pub_type： 撤回出版物
doi：10.1111/1556-4029.14292
更新日期：2020-05-01 00:00:00
abstract：:Photographic surveying is fundamental to crime scene investigation. It is performed using 2D images; however, this method is limited because it does not offer a detailed view. We present a 3D (three-dimensional) experimental model that has clarified unsolved aspects of a homicide. A woman was found dead in her home. C...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14041
更新日期：2019-09-01 00:00:00
abstract：:This case concerns a sudden death of a patient with Chiari I malformation. A 17-year-old female was seen unconscious then fell off a motorbike during the vehicle acceleration. The girl was confirmed dead on the way to hospital, being previously asymptomatic and with a clean medical record. Autopsy findings showed an e...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12051
更新日期：2013-03-01 00:00:00
abstract：:Forensic odontology cases examined from 2012 to 2019 at the Defense POW/MIA Accounting Agency (DPAA) laboratory were reviewed. Five cases from World War II and the Korea War were selected. Three of the cases presented involve dental assemblages, which were built by previous analysts. Postmortem radiographic analyses a...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14296
更新日期：2020-07-01 00:00:00
abstract：:The spreading dynamics of blood dripping onto hard surfaces is compared to two spreading models. Samples of human blood, porcine blood, and Millipore® water were dripped onto cardboard, foamcore, and glass surfaces in low velocity passive drip simulations. Final stain diameter, the total number of spines and scallops,...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13217
更新日期：2017-01-01 00:00:00
abstract：:A rapid, semiautomated radioimmunoassay system for detection of morphine, barbiturates, and amphetamines is described. The assays are applicable to large drug abuse screening programs. The heart of the system is the automatic pipetting station which can accomplish 600 pipetting operations per hour. The method uses 15 ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:In this paper, a novel three-dimensional transformation method for vehicle license plate number recognition is proposed. This method provides an efficient solution to normalize skew distorted vehicle license plate images. The Hough transform is used to estimate the license plate position and the normalization angle. A...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Prior to 1984, William R. Maples, Ph.D. worked primarily with Medical Examiners in the State of Florida in investigation of and testimony in criminal cases. In 1984 the Republic of Peru requested him to identify skeletal remains thought to be those of Francisco Pizarro, conqueror of Peru and the Incas in the early 16t...
journal_title：Journal of forensic sciences
pub_type： 传,历史文章,杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:This study investigated the postmortem diffusion of tracheal lidocaine into the blood after intubation in three individuals whose heart beat was not restored by cardiopulmonary resuscitation. The results are compared with those obtained in animal experiments using rabbits. The first human subject was a 3.5-month-old f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:We describe three unusual cases of suicide committed by health care workers. The aim of this paper was to analyze and evaluate the evidence of general diagnostic elements of poisoning in these cases. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:This report details the pathologic and toxicologic findings in the case of a 15-year-old girl who deliberately and fatally ingested brodifacoum, a commonly used rodenticide. The mechanism of death, massive pulmonary hemorrhage, has not been previously reported. Brodifacoum was quantitated in liver, spleen, lung, brain...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Handwriting of children in early grades is studied from the viewpoint of quantitatively measuring the development of handwriting individuality. Handwriting samples of children, in grades 2-4, writing a paragraph of text in both handprinted and cursive, collected over a period of 3 years, were analyzed using two differ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13158
更新日期：2016-09-01 00:00:00
abstract：:Vitreous humor chemistry profiles were reviewed on 133 autopsied cases in which death occurred outdoors during a six-year period, to determine whether environmental temperature at the time of death influenced chemistry values obtained at autopsy. The glucose concentration and total carbon dioxide content varied invers...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Measurements of the type and concentration of propellant and stabilizer additives in smokeless gunpowder are used by forensic scientists investigating the source of explosives and by military laboratories assuring the safety and efficacy of munitions. The National Institute of Standards and Technology recently assesse...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Seals are traditionally used in the Far East Asia to stamp an impression on a document in place of a signature. In this study, an accuser claimed that a personal contract regarding mining development rights acquired by a defendant was devolved to the accuser because the defendant stamped the devolvement contract in th...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12502
更新日期：2014-07-01 00:00:00
abstract：:A sexual assault case was received in the laboratory. Upon examination, a small bloodstain was located on a bed sheet that was recovered from the defendant's motel room. Typing the whole blood samples from the defendant and the victim revealed that both blood samples exhibited identical phenotypes in eleven different ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) restriction fragment length polymorphism (RFLP) profile results were obtained from bloodstains and other body fluid stains subjected to mixture with other body fluids, environmental insults (sunlight and temperature), different substrates (cotton, nylon, blue denim, glass, aluminum, and woo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:A principal goal of this research was to conduct a field evaluation of "on-site" multi-analyte drug testing devices to determine the most accurate, efficient, and cost-effective device available for the purpose of rapidly detecting drivers under the influence of drugs. Four on-site kits were selected and evaluated for...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Detection of ketamine exposure in skeletal tissues by automated enzyme-linked immunosorbent assay (ELISA) and gas chromatography with electron capture detection (GC-ECD) is described. Rats (n = 18) received 0, 15, 30, or 75 mg/kg ketamine hydrochloride acutely (i.p.), and were euthanized within 15 min or 1 h. Ketamine...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00866.x
更新日期：2008-11-01 00:00:00
abstract：:Using GC/MS in scanning mode as a screening and definitive identification methodology for substance abuse testing, 4500 urine samples have been analyzed. The accuracy and sensitivity of this method was evaluated by the results of 92 proficiency sample analyses, reanalyses by TLC screening with GC confirmation of 125 s...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
