Abstract:
PURPOSE:PCR is widely used for rapidly and accurately detecting Mycobacterium species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods. MATERIALS AND METHODS:Using 128 samples, the five PCR methods were assessed, including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis. RESULTS:For Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples. CONCLUSION:Real-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM.
journal_name
Yonsei Med Jjournal_title
Yonsei medical journalauthors
Cho SY,Kim MJ,Suh JT,Lee HJdoi
10.3349/ymj.2011.52.2.301subject
Has Abstractpub_date
2011-03-01 00:00:00pages
301-6issue
2eissn
0513-5796issn
1976-2437pii
201103301journal_volume
52pub_type
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1999.40.5.478
更新日期:1999-10-01 00:00:00
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
pub_type: 杂志文章,随机对照试验
doi:10.3349/ymj.2007.48.2.295
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1990.31.3.205
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2006.47.4.575
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.2003.44.2.288
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2016.57.2.523
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journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1992.33.3.258
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journal_title:Yonsei medical journal
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2000.41.1.123
更新日期:2000-02-01 00:00:00
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.1999.40.6.608
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.3.508
更新日期:2003-06-30 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1996.37.4.290
更新日期:1996-08-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.2.445
更新日期:2013-03-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1989.30.2.198
更新日期:1989-01-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.3.486
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.2.311
更新日期:2008-04-30 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.5.667
更新日期:2009-10-31 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.2.304
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