Optical probing of a dynamic membrane interaction that regulates the TREK1 channel.

abstract：:Sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase, EC 1.1.1.153) catalyzes the terminal step in the biosynthetic pathway for tetrahydrobiopterin, the cofactor necessary for aromatic amino acid hydroxylation. We report here the isolation of a cDNA clone for rat liver sepiapterin reductase. The cDNA has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Citron BA,Milstien S,Gutierrez JC,Levine RA,Yanak BL,Kaufman S

更新日期：1990-08-01 00:00:00

abstract：:Although it is known that the c-myc oncogene is rearranged in a head-to-head fashion with the immunoglobulin heavy chain locus in mouse plasmacytomas, it has not been clear whether the c-myc oncogene is translocated to the heavy chain locus on mouse chromosome 12 or whether the heavy chain locus is translocated to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Erikson J,Miller DA,Miller OJ,Abcarian PW,Skurla RM,Mushinski JF,Croce CM

更新日期：1985-06-01 00:00:00

abstract：:Contact of axons with target territories results in the formation of synapses, specific junctional complexes that may represent a final stage of neuronal maturation. Synaptosomal-associated protein 25 (SNAP-25) is a component of particular nerve terminals recently identified in rodent brain. To evaluate the structure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Catsicas S,Larhammar D,Blomqvist A,Sanna PP,Milner RJ,Wilson MC

更新日期：1991-02-01 00:00:00

abstract：:Placental abnormalities occur frequently in cloned animals. Here, we attempted to isolate trophoblast stem (TS) cells from mouse blastocysts produced by somatic cell nuclear transfer (NT) at the blastocyst stage (NT blastocysts). Despite the predicted deficiency of the trophoblast cell lineage, we succeeded in isolati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oda M,Tanaka S,Yamazaki Y,Ohta H,Iwatani M,Suzuki M,Ohgane J,Hattori N,Yanagimachi R,Wakayama T,Shiota K

更新日期：2009-09-22 00:00:00

abstract：:A nuclear protein, termed leucine-rich acidic nuclear protein (LANP), has been isolated from among rat cerebellar proteins whose expression was transiently increased during an early stage of postnatal development. The amino acid sequence, deduced from its cDNA, showed that LANP contains 247 amino acids consisting of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuoka K,Taoka M,Satozawa N,Nakayama H,Ichimura T,Takahashi N,Yamakuni T,Song SY,Isobe T

更新日期：1994-10-11 00:00:00

abstract：:Integrin-dependent adhesions mediate reciprocal exchange of force and information between the cell and the extracellular matrix. These effects are attributed to the "focal adhesion clutch," in which moving actin filaments transmit force to integrins via dynamic protein interactions. To elucidate these processes, we me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Driscoll TP,Ahn SJ,Huang B,Kumar A,Schwartz MA

更新日期：2020-12-22 00:00:00

abstract：:Smad proteins are key intracellular signaling effectors for the transforming growth factor-beta superfamily of peptide growth factors. Following receptor-induced activation, Smads move into the nucleus to activate transcription of a select set of target genes. The activity of Smad proteins must be tightly regulated to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Chang C,Gehling DJ,Hemmati-Brivanlou A,Derynck R

更新日期：2001-01-30 00:00:00

abstract：:In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuelsen CO,Baraznenok V,Khorosjutina O,Spahr H,Kieselbach T,Holmberg S,Gustafsson CM

更新日期：2003-05-27 00:00:00

abstract：:FtsZ, the ancestral homolog of eukaryotic tubulins, is a GTPase that assembles into a cytokinetic ring structure essential for cell division in prokaryotic cells. Similar to tubulin, purified FtsZ polymerizes into dynamic protofilaments in the presence of GTP; polymer assembly is accompanied by GTP hydrolysis. We used...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Margalit DN,Romberg L,Mets RB,Hebert AM,Mitchison TJ,Kirschner MW,RayChaudhuri D

更新日期：2004-08-10 00:00:00

abstract：:In biological processes, such as fission, fusion and trafficking, it has been shown that lipids of different shapes are sorted into regions with different membrane curvatures. This lipid sorting has been hypothesized to be due to the coupling between the membrane curvature and the lipid's spontaneous curvature, which ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamal MM,Mills D,Grzybek M,Howard J

更新日期：2009-12-29 00:00:00

abstract：:AAV is unique among eukaryotic viruses in the ability of its DNA to integrate preferentially into a specific region of the human genome. Understanding AAV integration may aid in developing gene therapy systems with predictable integration sites. Using a gel mobility-shift assay, we have identified a DNA sequence withi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weitzman MD,Kyöstiö SR,Kotin RM,Owens RA

更新日期：1994-06-21 00:00:00

abstract：:Compensatory ventilatory responses to increased inspiratory loading are essential for adequate breathing regulation in a number of pulmonary diseases; however, the human brain sites mediating such responses are unknown. Midsagittal and axial images were acquired in 11 healthy volunteers during unloaded and loaded (30 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gozal D,Omidvar O,Kirlew KA,Hathout GM,Hamilton R,Lufkin RB,Harper RM

更新日期：1995-07-03 00:00:00

abstract：:The tat protein encoded by the human immunodeficiency virus type 1 is a potent trans-activator of gene expression from the viral long terminal repeat. The domains that are essential for trans-activation, a Pro-Xaa3-Pro triad, a cysteine-rich metal-binding sequence motif, and a cluster of basic residues, are present wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jeyapaul J,Reddy MR,Khan SA

更新日期：1990-09-01 00:00:00

abstract：:A constitutively active form of mitogen-activated protein kinase kinase (MEK1) was synthesized under control of a zinc-inducible promoter in NIH 3T3 fibroblasts. Zinc treatment of serum-starved cells activated extracellular signal-regulated protein kinases (ERKs) and induced expression of cyclin D1. Newly synthesized ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng M,Sexl V,Sherr CJ,Roussel MF

更新日期：1998-02-03 00:00:00

abstract：:The specific nucleocytoplasmic compartmentalization of proteins has been examined for some major soluble acidic nuclear proteins in oocytes of different amphibia. Proteins synthesized and radioactively labeled by translation in vitro, by using mRNA from ovaries of the frog Xenopus laevis, were injected into the cytopl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabauvalle MC,Franke WW

更新日期：1982-09-01 00:00:00

abstract：:Axiomatic foundations for a theory of perception have been given in a prior communication [Shiman, L. G. (1978) Proc. Natl. Acad. Sci. USA 75, 2049-2053]. The theory is based on mathematically characterized stable images. Stable images model subjective (psychological) states of observers. We showed there is a universa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shiman LG

更新日期：1978-05-01 00:00:00

abstract：:Environmental stress often leads to an increased production of reactive oxygen species that are involved in plastid-to-nucleus retrograde signaling. Soon after the release of singlet oxygen ((1)O(2)) in chloroplasts of the flu mutant of Arabidopsis, reprogramming of nuclear gene expression reveals a rapid transfer of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simková K,Moreau F,Pawlak P,Vriet C,Baruah A,Alexandre C,Hennig L,Apel K,Laloi C

更新日期：2012-10-02 00:00:00

abstract：:The environmental topology of complex structures is used by Escherichia coli to create traveling waves of high cell density, a prelude to quorum sensing. When cells are grown to a moderate density within a confining microenvironment, these traveling waves of cell density allow the cells to find and collapse into confi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park S,Wolanin PM,Yuzbashyan EA,Lin H,Darnton NC,Stock JB,Silberzan P,Austin R

更新日期：2003-11-25 00:00:00

abstract：:In the hippocampus, a CA3 pyramidal cell forms excitatory synapses with thousands of other pyramidal cells and inhibitory interneurons. By using sequential paired recordings from three connected cells, we show that the presynaptic properties of CA3 pyramidal cell terminals, belonging to the same axon, differ according...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scanziani M,Gähwiler BH,Charpak S

更新日期：1998-09-29 00:00:00

abstract：:Targeted ablation of the surfactant protein D (SP-D) gene caused chronic inflammation, emphysema, and fibrosis in the lungs of SP-D (-/-) mice. Although lung morphology was unperturbed during the first 2 weeks of life, airspace enlargement was observed by 3 weeks and progressed with advancing age. Inflammation consist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wert SE,Yoshida M,LeVine AM,Ikegami M,Jones T,Ross GF,Fisher JH,Korfhagen TR,Whitsett JA

更新日期：2000-05-23 00:00:00

abstract：:Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvie NR,Schultz JS

更新日期：1970-05-01 00:00:00

abstract：:Lewy bodies and Lewy neurites are the defining neuropathological characteristics of Parkinson's disease and dementia with Lewy bodies. They are made of abnormal filamentous assemblies of unknown composition. We show here that Lewy bodies and Lewy neurites from Parkinson's disease and dementia with Lewy bodies are stai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spillantini MG,Crowther RA,Jakes R,Hasegawa M,Goedert M

更新日期：1998-05-26 00:00:00

abstract：:Glycoprotein Ib is a surface membrane glycoprotein of platelets that functions as a receptor for von Willebrand factor. It is a heterodimer composed of an alpha and a beta chain linked by a disulfide bond(s). A phage lambda gt11 cDNA expression library prepared from mRNA from a human erythroleukemia cell line, HEL, wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez JA,Chung DW,Fujikawa K,Hagen FS,Papayannopoulou T,Roth GJ

更新日期：1987-08-01 00:00:00

abstract：:Methylation on lysine 9 of histone H3 (H3K9me) and DNA methylation play important roles in the transcriptional silencing of specific genes and repetitive elements. Both marks are detected on class I and II endogenous retroviruses (ERVs) in murine embryonic stem cells (mESCs). Recently, we reported that the H3K9-specif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung DC,Dong KB,Maksakova IA,Goyal P,Appanah R,Lee S,Tachibana M,Shinkai Y,Lehnertz B,Mager DL,Rossi F,Lorincz MC

更新日期：2011-04-05 00:00:00

abstract：:Recent sequencing efforts have led to estimates of human cytomegalovirus (HCMV) genome-wide intrahost diversity that rival those of persistent RNA viruses [Renzette N, Bhattacharjee B, Jensen JD, Gibson L, Kowalik TF (2011) PLoS Pathog 7:e1001344]. Here, we deep sequence HCMV genomes recovered from single and longitud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cudini J,Roy S,Houldcroft CJ,Bryant JM,Depledge DP,Tutill H,Veys P,Williams R,Worth AJJ,Tamuri AU,Goldstein RA,Breuer J

更新日期：2019-03-19 00:00:00

abstract：:The Arabidopsis thaliana disease resistance genes RPS2 and RPM1 belong to a class of plant disease resistance genes that encode proteins that contain an N-terminal tripartite nucleotide binding site (NBS) and a C-terminal tandem array of leucine-rich repeats. RPS2 and RPM1 confer resistance to strains of the bacterial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leister RT,Ausubel FM,Katagiri F

更新日期：1996-12-24 00:00:00

abstract：:Human T lymphocytes were shown to proliferate in response to tetanus toxoid antigen only in the presence of macrophages. This response was inhibited by anti-DRw but not by anti-HLA (A and B loci) antisera added to the cultures and by pretreatment of macrophages but not of T cells with anti-DRw antisera and complement....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geha RS,Milgrom H,Broff M,Alpert S,Martin S,Yunis EJ

更新日期：1979-08-01 00:00:00

abstract：:Because previous studies showed that polyunsaturated fatty acids can reduce the contraction rate of spontaneously beating heart cells and have antiarrhythmic effects, we examined the effects of the fatty acids on the electrophysiology of the cardiac cycle in isolated neonatal rat cardiac myocytes. Exposure of cardiomy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang JX,Xiao YF,Leaf A

更新日期：1995-04-25 00:00:00

abstract：:Recent excavations at Pre-Pottery Neolithic A (PPNA) WF16 in southern Jordan have revealed remarkable evidence of architectural developments in the early Neolithic. This sheds light on both special purpose structures and "domestic" settlement, allowing fresh insights into the development of increasingly sedentary comm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finlayson B,Mithen SJ,Najjar M,Smith S,Maričević D,Pankhurst N,Yeomans L

更新日期：2011-05-17 00:00:00

abstract：:The crossed strand-exchange form (Holliday structure, half chiasma) has been predicted as an intermediate in the genetic recombination of eukaryotes. We report here the detection of this form in the yeast plasmid, 2-micron DNA, isolated during meiosis. Physical mapping has previously suggested that two forms of 2-micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bell L,Byers B

更新日期：1979-07-01 00:00:00