Abstract:
:We have previously shown that Wnt5A-mediated signaling can promote melanoma metastasis. It has been shown that Wnt signaling is antagonized by the protein Klotho, which has been implicated in aging. We show here that in melanoma cells, expressions of Wnt5A and Klotho are inversely correlated. In the presence of recombinant Klotho (rKlotho), we show that Wnt5A internalization and signaling is decreased in high Wnt5A-expressing cells. Moreover, in the presence of rKlotho, we observe an increase in Wnt5A remaining in the medium, coincident with an increase in sialidase activity, and decrease in syndecan expression. These effects can be inhibited using a sialidase inhibitor. In addition to its effects on Wnt5A internalization, we also demonstrate that Klotho decreases melanoma cell invasive potential by a second mechanism that involves the inhibition of calpain and a resultant decrease in filamin cleavage, which we demonstrate is critical for melanoma cell motility.
journal_name
Pigment Cell Melanoma Resjournal_title
Pigment cell & melanoma researchauthors
Camilli TC,Xu M,O'Connell MP,Chien B,Frank BP,Subaran S,Indig FE,Morin PJ,Hewitt SM,Weeraratna ATdoi
10.1111/j.1755-148X.2010.00792.xsubject
Has Abstractpub_date
2011-02-01 00:00:00pages
175-86issue
1eissn
1755-1471issn
1755-148Xjournal_volume
24pub_type
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
pub_type: 杂志文章,评审
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
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journal_title:Pigment cell & melanoma research
pub_type: 杂志文章,评审
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