Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.

Abstract:

AIM:To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS:A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS:Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION:A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.

journal_name

World J Gastroenterol

authors

Karagin PH,Stenram U,Wadström T,Ljungh A

doi

10.3748/wjg.v16.i38.4817

subject

Has Abstract

pub_date

2010-10-14 00:00:00

pages

4817-22

issue

38

eissn

1007-9327

issn

2219-2840

journal_volume

16

pub_type

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