Fixation methods for electron microscopy of human and other liver.

Abstract:

:For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.

journal_name

World J Gastroenterol

authors

Wisse E,Braet F,Duimel H,Vreuls C,Koek G,Olde Damink SW,van den Broek MA,De Geest B,Dejong CH,Tateno C,Frederik P

doi

10.3748/wjg.v16.i23.2851

subject

Has Abstract

pub_date

2010-06-21 00:00:00

pages

2851-66

issue

23

eissn

1007-9327

issn

2219-2840

journal_volume

16

pub_type

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