Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods.

Abstract:

OBJECTIVE:Infection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia. METHOD OF STUDY:Amniotic fluid (AF) from 62 subjects with preeclampsia, not in labor, was analyzed with both cultivation and molecular methods. Broad-range and group-specific PCR assays targeting small subunit ribosomal DNA, or other gene sequences, from bacteria, fungi and archaea were used. Results were correlated with measurements of host inflammatory response, including AF white blood cell count and AF concentrations of glucose, interleukin-6 (IL-6) and MMP-8. RESULTS:1) The rate of MIAC in preeclampsia was 1.6% (1/62) based on cultivation techniques, 8% (5/62) based on PCR, and 9.6% (6/62) based on the combined results of both methods; 2) among the six patients diagnosed with MIAC, three had a positive PCR for Sneathia/Leptotrichia spp.; and 3) patients with MIAC were more likely to have evidence of an inflammatory response in the amniotic cavity than those without MIAC, as determined by a higher median AF IL-6 [1.65 ng/mL interquartile range (IQR): 0.35-4.62 vs. 0.22 ng/mL IQR: 0.12-0.51; P=0.002). CONCLUSION:The prevalence of MIAC in preeclampsia is low, suggesting that intra-amniotic infection plays only a limited role in preeclampsia. However, the unexpectedly high number of positive AF specimens for Sneathia/Leptotrichia warrants further investigation.

journal_name

J Perinat Med

authors

DiGiulio DB,Gervasi M,Romero R,Mazaki-Tovi S,Vaisbuch E,Kusanovic JP,Seok KS,Gómez R,Mittal P,Gotsch F,Chaiworapongsa T,Oyarzún E,Kim CJ,Relman DA

doi

10.1515/jpm.2010.078

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

503-13

issue

5

eissn

0300-5577

issn

1619-3997

journal_volume

38

pub_type

杂志文章
  • To 'C' or not to 'C'? Caesarean delivery upon maternal request: a review of facts, figures and guidelines.

    abstract::The last few decades have seen an unrelenting rise in caesarean section (CS) rates. In addition to an increase in numbers of CS performed worldwide, there has also been a change in the indications for CS, a reflection of changing times. A new dilemma facing obstetricians is the increasing demand for CS in the absence ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/jpm-2012-0049

    authors: D'Souza R,Arulkumaran S

    更新日期:2013-01-01 00:00:00

  • Impact of breast-feeding on psychomotor and neuropsychological development in children of diabetic mothers: role of the late neonatal period.

    abstract:AIM:Previous data from our Kaulsdorf Cohort Study (KCS) suggest that early neonatal ingestion (1st week) of breast milk from diabetic mothers (diabetic breast milk, DBM) may increase the risk of being overweight and delay speech development in offspring of diabetic mothers (ODM). Late neonatal DBM ingestion (2nd-4th we...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2006.095

    authors: Rodekamp E,Harder T,Kohlhoff R,Dudenhausen JW,Plagemann A

    更新日期:2006-01-01 00:00:00

  • The concentration of bupivacaine in fetal organs during obstetrical epidural analgesia.

    abstract::The concentration of bupivacaine in organs of non-viable human fetuses after obstetrical epidural analgesia in customary doses was investigated. The concentrations were determined with a gas chromatograph. The material consisted of a fetus who died following termination in the 24th week of pregnancy three minutes afte...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1986.14.4.219

    authors: Müller-Holve W,von Meyer L,Nagler P

    更新日期:1986-01-01 00:00:00

  • Clinical characteristics of nosocomial infections in neonatal intensive care unit in eastern China.

    abstract::A retrospective cohort study on nosocomial infections (NI) in the neonatal intensive care unit (NICU) was performed in the Children's Hospital of Zhejiang University, Hangzhou district, China. The most common infection site was pneumonia and bloodstream infection. Low admission age, long NICU stay, and mechanical vent...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpm.2010.063

    authors: Xu XF,Ma XL,Chen Z,Shi LP,Du LZ

    更新日期:2010-07-01 00:00:00

  • Non-selective fetal reduction is malpractice.

    abstract::Non-selective multifetal pregnancy reduction is carried out to reduce healthy higher order multiple fetuses to one or two fetuses. No studies exist to show any benefit of this practice and a Cochrane review, as well as investigators in the field, have not found any justification for such practice. From a medical point...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/JPM.2006.072

    authors: Saugstad OD

    更新日期:2006-01-01 00:00:00

  • Ventricular dominance patterns in preterm infants.

    abstract:OBJECTIVE:The purpose of the study was to determine EKG and 2-D echocardiographic criteria of ventricular dominance in preterm infants and select those by which ventricular dominance could be established by EKG alone. METHODS:A database was constructed from EKG and 2-D echocardiographic measurements on preterm infants...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.1999.040

    authors: Schmer V,Mogos C,Gudavalli M,Sutija VG,Tugertimur A

    更新日期:1999-01-01 00:00:00

  • New approaches to the prediction of preterm delivery.

    abstract::Current approaches to the detection of patients at risk for preterm delivery (PTD) have focused on the diagnosis of preterm labor. However, these approaches are complicated by treatment biases and our inability to convincingly differentiate preterm labor from Braxton-Hicks contractions. Moreover, preterm labor with in...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/jpme.1993.21.6.441

    authors: Lockwood CJ,Dudenhausen JW

    更新日期:1993-01-01 00:00:00

  • MRI during pregnancy.

    abstract::The use of ultrasonography (USG) during pregnancy provides a tremendous amount of valuable information about fetal and maternal well-being. However, in some cases the image quality may be poor due to maternal fat tissue, fetal position, or some other reason. Magnetic resonance imaging (MRI) can provide clear images fr...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/JPM.2003.070

    authors: Kawabata I,Takahashi Y,Iwagaki S,Tamaya T

    更新日期:2003-01-01 00:00:00

  • The cisterna magna size in normal second-trimester fetuses.

    abstract:OBJECTIVE:The purpose of this study was to evaluate cisterna magna size of the fetuses between 16 and 24 weeks of gestation, and to establish a normogram for cisterna magna measurements during gestation. METHODS:A prospective study of normal singleton pregnancies was established. Measurement of the fetal cisterna magn...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2007.060

    authors: Koktener A,Dilmen G,Kurt A

    更新日期:2007-01-01 00:00:00

  • The role of ultrasonography in recognizing the cause of fetal cerebral ventriculomegaly.

    abstract::Dilatation of the fetal cerebral ventricles (ventriculomegaly) is a generic sonographic sign common to several pathological entities carrying different prognoses. The main causes of fetal ventriculomegaly are aqueductal stenosis, Chiari II malformation, Dandy-Walker complex and agenesis of the corpus callosum. Ventric...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/JPM.2004.002

    authors: D'Addario V

    更新日期:2004-01-01 00:00:00

  • Does rescue cerclage work?

    abstract::Rescue cervical cerclage (RCC), also known as emergency cerclage, has long been the subject of controversy. Its use in women who have a dilated cervix has been ambivalent. RCC is often considered as a salvage measure for pregnancies which are at a high risk of severe preterm delivery (PTD) or mid-trimester miscarriage...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpm-2017-0311

    authors: Shivani D,Quek BH,Tan PL,Shephali T

    更新日期:2018-10-25 00:00:00

  • Duration of the multifetal gestation, birth weight and infant prognosis.

    abstract::For the higher numbered multifetal gestation, duration of gestational age decreases in spite of different medical activities. On the other hand increasing gestational age often strongly suggests the need for delivery by caesarean section for maternal indications. After the 32nd week of gestation in general no serious ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1993.21.4.295

    authors: Knitza R,Ott M,Hasbargen U,Hepp H

    更新日期:1993-01-01 00:00:00

  • Maternal serum lipid profile in pregnancies complicated by hypertensive disorders.

    abstract::Plasma lipoprotein, cholesterol and triglycerides were determined in 34 hypertensive pregnant patients and in 17 healthy full term pregnant women. Pregnancy induced hypertension was diagnosed in 21 patients and chronic hypertension in the remaining 13 women. Serum triglyceride levels were significantly elevated in the...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1990.18.6.473

    authors: Kokia E,Barkai G,Reichman B,Segal P,Goldman B,Mashiach S

    更新日期:1990-01-01 00:00:00

  • Prenatal sonographic diagnosis of congenital sacrococcygeal teratoma and management.

    abstract::Teratomas are tumors containing tissues derived from all three germinal cell layers. Most teratomas arise in the sacrococcygeal region, with a reported incidence of 1/40,000 births. During labor, severe dystocia may occur in infants with large tumors if delivered vaginally, with an extremely high fetal morbidity and m...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1989.17.2.93

    authors: Sepúlveda WH

    更新日期:1989-01-01 00:00:00

  • Perinatal mortality and risk of delivery. What's happening in Italy?

    abstract::The aim of this paper is to establish the exact incidence of risk in delivery as related to perinatal mortality rate. The entire 1976 Italian population was statistically sampled (latest data available), specifically enucleating the sanitary data reported in 1976 by ISTAT (National Institute of Statistics) for the var...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1982.10.6.293

    authors: Selvaggi L,di Vagno G

    更新日期:1982-01-01 00:00:00

  • Ranitidine and late-onset sepsis in the neonatal intensive care unit.

    abstract:AIMS:The objective of the study was to examine the effect of ranitidine on the incidence of late onset sepsis. METHODS:This study was based on information extracted from charts of 569 infants admitted to the neonatal intensive care unit (NICU) from July 2003 to July 2005. All infants admitted for seven or more days we...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2007.017

    authors: Bianconi S,Gudavalli M,Sutija VG,Lopez AL,Barillas-Arias L,Ron N

    更新日期:2007-01-01 00:00:00

  • Sudden intractable respiratory failure in extremely low birth weight infants with H-type tracheoesophageal fistula.

    abstract::A fatality from a tracheoesophageal fistula (TEF) in two extremely low birth weight infants is presented. The sudden onset of intractable respiratory failure accompanied by the absence of chest movement and breathing sounds was observed. The typical clinical symptoms were concealed because the infants required mechani...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2002.037

    authors: Hosono S,Fuyama Y,Ohno T,Kishimoto H,Ogawa Y

    更新日期:2002-01-01 00:00:00

  • Nomogram of the fetal gastric size development in normal pregnancy.

    abstract:OBJECTIVE:To construct an ultrasonographic nomogram of the fetal gastric size with gestational age in normal singleton pregnancy. PATIENTS AND METHODS:Out of 17 000 outpatient pregnant women who were admitted to our hospital, a total of 250 fetal measurements were randomly collected from routine ultrasonographic exami...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2005.060

    authors: Kepkep K,Tuncay YA,Göynümer G,Yetim G

    更新日期:2005-01-01 00:00:00

  • The frequency and clinical significance of intra-amniotic infection and/or inflammation in women with placenta previa and vaginal bleeding: an unexpected observation.

    abstract:OBJECTIVE:Idiopathic vaginal bleeding, a common complication of pregnancy, increases the risk of small-for-gestational age (SGA) neonate, preeclampsia and preterm delivery and can be the only clinical manifestation of intra-amniotic infection and/or inflammation (IAI). Placenta previa is thought to be protective agains...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpm.2010.001

    authors: Madan I,Romero R,Kusanovic JP,Mittal P,Chaiworapongsa T,Dong Z,Mazaki-Tovi S,Vaisbuch E,Alpay Savasan Z,Yeo L,Kim CJ,Hassan SS

    更新日期:2010-05-01 00:00:00

  • Observations on the energy and redox state and protein synthetic rate in animal and human placentas.

    abstract::The energy and redox states of placentas in newly anesthetized or sacrificed guinea pigs and sheep were compared with that of human placentas immediately after delivery. Subsequently the effect of ischemia was observed in both human and animal placentas. The influence of perfusion, using a fluid containing oxygenated ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1987.15.1.21

    authors: Carroll MJ,Young M

    更新日期:1987-01-01 00:00:00

  • The impact of length of second stage of labor on shoulder dystocia outcomes: a retrospective cohort study.

    abstract:OBJECTIVE:To compare obstetrical and neonatal outcomes of vaginal deliveries complicated by shoulder dystocia, according to the length of second stage of labor. METHODS:We conducted a retrospective cohort study of 177 shoulder dystocia cases that were divided into three categories according to second stage duration (1...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpm-2011-0253

    authors: Moragianni VA,Hacker MR,Craparo FJ

    更新日期:2012-03-29 00:00:00

  • Risk factors and outcomes for ventilator-associated pneumonia in neonatal intensive care unit patients.

    abstract::In order to determine the risk factors and outcomes of ventilator-associated pneumonia (VAP) in the neonatal intensive care unit (NICU), a retrospective cohort study was conducted on 259 patients who were ventilated >48 h. Clinical characteristics and risk factors were compared and non-conditional logistic regression ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2007.065

    authors: Yuan TM,Chen LH,Yu HM

    更新日期:2007-01-01 00:00:00

  • Neonatal sepsis due to echovirus 18 infection.

    abstract::Clinical manifestations of neonatal echovirus type 18 infections include a nonspecific febrile illness, diarrhea, and meningitis with or without exanthem. We report a successful outcome in a case of neonatal sepsis with shock caused by echovirus type 18, a complication not previously associated with this serotype. ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1997.25.4.381

    authors: Shah SS,Gallagher PG

    更新日期:1997-01-01 00:00:00

  • Plasma angiotensin II and blood pressure changes during dietary sodium manipulation.

    abstract::The renin-angiotensin system and dietary sodium have been repeatedly implicated in the pathogenesis of pregnancy induced hypertension (PIH). Mechanisms responsible for increased vascular responsiveness to angiotensin II (A-II) observed in PIH are not completely understood. Plasma A-II levels and blood pressure are fun...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1984.12.4.167

    authors: Lee MI,Sokol RJ,Todd H

    更新日期:1984-01-01 00:00:00

  • Maturation of the autonomic nervous system: differences in heart rate variability in premature vs. term infants.

    abstract:AIMS:Heart rate variability (HRV) reveals information on the functional state of the autonomic nervous system (ANS). This study was initiated to assess the physiological- and maturational development of the ANS by comparing HRV data of healthy prematures with term infants. METHODS:Short-term recordings of HRV in 39 pr...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2006.058

    authors: Longin E,Gerstner T,Schaible T,Lenz T,König S

    更新日期:2006-01-01 00:00:00

  • Bronchopulmonary dysplasia and early prophylactic inhaled nitric oxide in preterm infants: current concepts and future research strategies in animal models.

    abstract::We reviewed the literature on the use of inhaled nitric oxide and the influence of supplemental oxygen on bronchopulmonary dysplasia (BPD), and the role of endogenous nitric oxide-synthase, vascular endothelial growth factor, the interplay of nitric oxide and superoxide, protein nitration and the nuclear factor kappa ...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/JPM.2008.065

    authors: Rieger-Fackeldey E,Hentschel R

    更新日期:2008-01-01 00:00:00

  • Diameters of the common carotid artery and aorta change in different directions during acute asphyxia in the fetal lamb.

    abstract::The aim of this experimental ultrasound study on six fetal lambs was to evaluate how blood flow variables and vessel diameters of the descending aorta and the common carotid artery change during fetal asphyxia in the acute preparation. When acute asphyxia was induced by obstructing the maternal aortic blood flow all f...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:

    authors: Malcus P,Kjellmer I,Lingman G,Marsál K,Thiringer K,Rosén KG

    更新日期:1991-01-01 00:00:00

  • New scoring system for fetal neurobehavior assessed by three- and four-dimensional sonography.

    abstract:AIM:To produce a new scoring system for fetal neurobehavior based on prenatal assessment by 3D/4D sonography. We identified severely brain damaged infants and those with optimal neurological findings and compared fetal with neonatal findings. RESULTS:The new scoring system was retrospectively applied in a group of 100...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/JPM.2008.007

    authors: Kurjak A,Miskovic B,Stanojevic M,Amiel-Tison C,Ahmed B,Azumendi G,Vasilj O,Andonotopo W,Turudic T,Salihagic-Kadic A

    更新日期:2008-01-01 00:00:00

  • The neonatal bisacromial diameter: a prenatal sonographic evaluation.

    abstract::This study was designed to determine which fetal ultrasonographic parameter best correlates with the neonatal bisacromial diameter (BAD). The latter is defined as the distance between the two acromions of the scapulae. The study population included fifty-four uncomplicated singleton pregnant patients whose infants wit...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章

    doi:10.1515/jpme.1997.25.6.484

    authors: Winn HN,Holcomb W,Shumway JB,al-Malt A,Amon E,Hobbins JC

    更新日期:1997-01-01 00:00:00

  • The application of individualised fetal growth curves.

    abstract::Individually adjusted or 'customised' growth charts aim to optimise the assessment of fetal growth by taking individual variation into account, and by projecting an optimal curve which delineates the potential weight gain in each pregnancy. This results in an increased detection rate of true growth restriction and a r...

    journal_title:Journal of perinatal medicine

    pub_type: 杂志文章,评审

    doi:10.1515/jpme.1998.26.4.333

    authors: Gardosi J

    更新日期:1998-01-01 00:00:00