Transformation of Phomopsis viticola with the green fluorescent protein.

Abstract:

:Phomopsis viticola is the causal agent of Phomopsis cane and leaf spot on Vitis spp., a persistent and economically important disease in temperate regions. Here we describe the transformation of this fungus with two different constructs (pBHt2_sGFP and pIGPAPA) containing the green fluorescent protein (GFP) and the hygromycin B resistance gene (hph). Protoplast-mediated transformation yielded mitotically stable transformants with no change in virulence on grape internodes and leaves in comparison to the wild type. These transformants will be critical tools for elucidating fungal penetration of host plants, invasive growth and the nature of its host association.

journal_name

Mycologia

journal_title

Mycologia

authors

Anco DJ,Kim S,Mitchell TK,Madden LV,Ellis MA

doi

10.3852/09-007

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

853-8

issue

6

eissn

0027-5514

issn

1557-2536

journal_volume

101

pub_type

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