Abstract:
:A rapid immunohistochemical method using 5'-bromo-2'-deoxyuridine (BrdU), a thymidine analog, for labeling proliferating epithelial cells was modified and tested for accuracy against standard tritiated thymidine autoradiography (3H-TdR) in rat esophagus, stomach, duodenum, and colon. Either BrdU or 3H-thymidine or both compounds (simultaneously) were injected IP. Histological sections of these tissues were immunostained with monoclonal anti-BrdU antibody, linked to horseradish peroxidase by standard avidin-biotin techniques and stained with diaminobenzidine, or sections were dipped for autoradiography, or both techniques were applied to the same tissue section. Results showed that (a) BrdU labeled the same number of proliferating cells in all organs as 3H-TdR; (b) BrdU colabeled with 3H-thymidine; (c) immunostaining was complete in 3-4 days but standard 3H-TdR took 2 weeks; and (d) qualitative analysis took 50% less time with BrdU than with standard 3H-TdR.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Lacy ER,Kuwayama H,Cowart KS,King JS,Deutz AH,Sistrunk Sdoi
10.1016/0016-5085(91)90610-wsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
259-62issue
1eissn
0016-5085issn
1528-0012pii
0016-5085(91)90610-Wjournal_volume
100pub_type
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