Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge.

Abstract:

:Two novel anaerobic, moderately thermophilic and cellulose-/cellobiose-digesting bacteria, EBR45(T) and EBR596(T), were isolated from anaerobic sludge of a cellulose-degrading methanogenic bioreactor. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belonged to cluster III within the low-G+C-content Gram-positive bacteria. The close relatives of EBR45(T) were Clostridium straminisolvens DSM 16021(T) (sequence identity, 94.6 %) and Clostridium thermocellum DSM 1237(T) (93.4 %). The closest relative of EBR596(T) was Clostridium stercorarium DSM 8532(T) (95.9 %). Both isolates were rod-shaped sporulators, growing optimally at 60 degrees C. EBR45(T) was Gram-staining-reaction-variable and non-motile, formed bright-yellow colonies on solid media, and grew on a relatively narrow range of carbohydrates including cellulose and cellobiose. EBR596(T) was Gram-staining-reaction-negative and motile, formed glossy white colonies and grew on cellobiose and various carbohydrates except cellulose. Major fatty acid compositions were 16 : 0 iso, 16 : 0 and 16 : 0 dimethylacetal (strain EBR45(T)) and 15 : 0 iso, 16 : 0 iso, 15 : 0 anteiso and 17 : 0 anteiso (strain EBR596(T)). The DNA G+C contents were 36.9 mol% (EBR45(T)) and 51.1 mol% (EBR596(T)). Based on the phenotypic and phylogenetic data and genomic distinctiveness, strains EBR45(T) and EBR596(T) represent two novel species, for which the names Clostridium clariflavum sp. nov. (type strain EBR45(T) =DSM 19732(T) =NBRC 101661(T)) and Clostridium caenicola sp. nov. (type strain EBR596(T) =DSM 19027(T) =NBRC 102590(T)) are proposed.

authors

Shiratori H,Sasaya K,Ohiwa H,Ikeno H,Ayame S,Kataoka N,Miya A,Beppu T,Ueda K

doi

10.1099/ijs.0.003483-0

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

1764-70

issue

Pt 7

eissn

1466-5026

issn

1466-5034

pii

ijs.0.003483-0

journal_volume

59

pub_type

杂志文章
  • Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China.

    abstract::Three chickpea rhizobial strains (WYCCWR 10195T=S1-3-7, WYCCWR 10198=S1-4-3 and WYCCWR 10200=S1-5-1) isolated from Northwest China formed a group affiliated to Mesorhizobium based on 16S rRNA gene sequence comparison. To clarify their species status, multilocus sequence analysis and average nucleotide identity (ANI) v...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002770

    authors: Zhang J,Guo C,Chen W,de Lajudie P,Zhang Z,Shang Y,Wang ET

    更新日期:2018-06-01 00:00:00

  • Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

    abstract::Eleven strains from hamster of Bisgaard taxa 23 and 24, also referred to as Krause's groups 2 and 1, respectively, were investigated by a polyphasic approach including data published previously. Strains showed small, regular and circular colonies with smooth and shiny appearance, typical of members of the family Paste...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.067470-0

    authors: Christensen H,Nicklas W,Bisgaard M

    更新日期:2014-11-01 00:00:00

  • Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Malaga, southern Spain.

    abstract::Three Halomonas strains, FP34, FP35T and FP36, which were isolated from soil samples taken from Fuente de Piedra, a saline wetland in the province of Málaga in southern Spain, are described. Phylogenetic analyses based on 16S rRNA gene sequences show that the three isolates belong to the genus Halomonas in the gamma-P...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63108-0

    authors: Martínez-Cánovas MJ,Béjar V,Martínez-Checa F,Quesada E

    更新日期:2004-07-01 00:00:00

  • Genomic insights into a novel species Rhodoferax aquaticus sp. nov., isolated from freshwater.

    abstract::A novel non-phototrophic member of the genus Rhodoferax was obtained from freshwater. The purpose of this study was to analyse the genome of a nonphototrophic strain and propose a new species based on its phylogenetic, genomic, physiological and chemotaxonomic characteristics. The results of phylogenetic analysis base...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004325

    authors: Li T,Zhuo Y,Jin CZ,Wu X,Ko SR,Jin FJ,Ahn CY,Oh HM,Lee HG,Jin L

    更新日期:2020-08-01 00:00:00

  • Ketobacter nezhaii sp. nov., a marine bacterium isolated from coastal sediment.

    abstract::A Gram-stain-negative, motile, aerobic and heterotrophic bacterium, designated as GYS_M3HT, was isolated from marine coastal sediment sampled at Xiamen Island. Cells were rod-shaped with one polar flagellum and weakly positive for oxidase and catalase. Growth of the strain occurred at pH 6-9 (optimum, pH 7-8), at 15-3...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004365

    authors: Li J,Qi M,Lai Q,Liu X,Shao Z

    更新日期:2020-09-01 00:00:00

  • Deinococcus metallilatus sp. nov. and Deinococcus carri sp. nov., isolated from a car air-conditioning system.

    abstract::Two bacterial strains, designated MA1002(T) and MA1003(T), were isolated from the air-conditioning system of a car. Cells of both strains were Gram-reaction-positive, non-motile, non-spore-forming coccoids, catalase- and oxidase-positive and UV-radiation resistant. The major fatty acids of strain MA1002(T) were iso-C1...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000396

    authors: Kim DU,Lee H,Lee JH,Ahn JH,Lim S,Jeong S,Park SY,Seong CN,Ka JO

    更新日期:2015-09-01 00:00:00

  • Mucilaginibacter roseus sp. nov., isolated from a freshwater river.

    abstract::A bacterial strain, designated TTM-1T, was isolated from a water sample taken from the Caohu River in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain TTM-1T were Gram-stain-negative, aerobic, non-motile, rod-shaped and covered by large capsules, and formed pink-coloured colon...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000838

    authors: Chen WM,Chen YL,Sheu SY

    更新日期:2016-03-01 00:00:00

  • Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.

    abstract::Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (Mars Odyssey Orbiter) and assembly-facility surfaces at the Jet Propulsion Laboratory in California and the Kennedy Space Center in Florida. Phylogenetic analysis of 16S rRNA gene sequen...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64189-0

    authors: Satomi M,La Duc MT,Venkateswaran K

    更新日期:2006-08-01 00:00:00

  • Vibrio rhizosphaerae sp. nov., a red-pigmented bacterium that antagonizes phytopathogenic bacteria.

    abstract::Two novel red-pigmented Vibrio strains, MSSRF3(T) and MSSRF10, with antibacterial activity against phytopathogens were isolated from the rhizosphere region of mangrove-associated wild rice (Porteresia coarctata Tateoka), in Pichavaram, India. The cells were Gram-negative, facultatively anaerobic and rod-shaped and wer...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65017-0

    authors: Kumar NR,Nair S

    更新日期:2007-10-01 00:00:00

  • Streptomyces specialis sp. nov.

    abstract::A Gram-positive, non-endospore-forming bacterium (GW41-1564(T)) was isolated from soil. Comparison of 16S rRNA gene sequences showed that strain GW41-1564(T) is a member of the genus Streptomyces, exhibiting highest similarities with Streptomyces hainanensis YIM 47672(T) (97.8 %) and Streptomyces cacaoi subsp. cacaoi ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.2008/001008-0

    authors: Kämpfer P,Huber B,Buczolits S,Thummes K,Grün-Wollny I,Busse HJ

    更新日期:2008-11-01 00:00:00

  • Ochrobactrum pituitosum sp. nov., isolated from an industrial environment.

    abstract::Strain CCUG 50899, a Gram-negative, rod-shaped, non-spore-forming, motile bacterium isolated from industrial environment in Sweden and tentatively assigned to the species Ochrobactrum anthropi, was studied in order to clarify its taxonomic status. 16S rRNA gene sequence similarities placed the strain in the genus Ochr...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.011668-0

    authors: Huber B,Scholz HC,Kämpfer P,Falsen E,Langer S,Busse HJ

    更新日期:2010-02-01 00:00:00

  • Arthrobacter deserti sp. nov., isolated from a desert soil sample.

    abstract::A Gram-stain-positive, non-motile, rod-shaped, catalase-positive and oxidase-negative bacterium, designated YIM CS25T, was isolated from a soil sample collected from Turpan desert in Xinjiang Uyghur Autonomous Region, north-western China. The isolate grew at 15-40 °C, at pH 6.0-8.0 and with 0-6 % (w/v) NaCl. The phylo...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000986

    authors: Hu QW,Chu X,Xiao M,Li CT,Yan ZF,Hozzein WN,Kim CJ,Zhi XY,Li WJ

    更新日期:2016-05-01 00:00:00

  • Falsibacillus albus sp. nov., isolated from mangrove soil.

    abstract::A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China. Cells were aerobic, motile with peritrichous flagella and rod-shaped. The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003328

    authors: Shi SB,Liu C,Jiang MG,Li GD,Yang LF,Wu JF,Jiang LQ,Zhang K,Shen NK,Jiang CL,Jiang Y

    更新日期:2019-05-01 00:00:00

  • Microbacterium excoecariae sp. nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.

    abstract::A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China. Cells were short rods. Colonies were light yellow, circular and had entire margins. Strain CBS5P-1T grew at 10-37 °C (optimum, 30 °C...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004523

    authors: Chen MS,Li F,Yan XR,Tuo L

    更新日期:2020-12-01 00:00:00

  • Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil.

    abstract::A novel bacterium, strain Br(T), was isolated from gamma-irradiated soils of the Britannia drift, Lake Wellman Region, Antarctica. This isolate was rod-shaped, endospore forming, Gram-stain-variable, catalase-positive, oxidase-negative and strictly aerobic. Cells possessed a monotrichous flagellum. Optimal growth was ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.056697-0

    authors: Dsouza M,Taylor MW,Ryan J,MacKenzie A,Lagutin K,Anderson RF,Turner SJ,Aislabie J

    更新日期:2014-04-01 00:00:00

  • Terrimonas pekingensis sp. nov., isolated from bulking sludge, and emended descriptions of the genus Terrimonas, Terrimonas ferruginea, Terrimonas lutea and Terrimonas aquatica.

    abstract::A Gram-negative, strictly aerobic, non-motile and non-spore-forming rod that produced white, viscous colonies, designated QH(T), was isolated from bulking sludge collected from a municipal wastewater treatment plant in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QH(T) belo...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.036848-0

    authors: Jin D,Wang P,Bai Z,Jin B,Yu Z,Wang X,Zhuang G,Zhang H

    更新日期:2013-05-01 00:00:00

  • Aquipuribacter hungaricus gen. nov., sp. nov., an actinobacterium isolated from the ultrapure water system of a power plant.

    abstract::A Gram-positive actinobacterium, strain IV-75(T), was isolated by using R2A agar from the ultrapure water system of a power plant in Hungary. The strain exhibited a rod-coccus cell cycle, and was strictly aerobic, non-motile, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that strain ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.032672-0

    authors: Tóth EM,Kéki Z,Bohus V,Borsodi AK,Márialigeti K,Schumann P

    更新日期:2012-03-01 00:00:00

  • Nemorella caseinilytica gen. nov., sp. nov., isolated from forest soil.

    abstract::Two novel strains, J116-2T and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. St...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002427

    authors: Chaudhary DK,Dahal RH,Kim J

    更新日期:2018-02-01 00:00:00

  • Marmoricola solisilvae sp. nov. and Marmoricola terrae sp. nov., isolated from soil and emended description of the genus Marmoricola.

    abstract::Two strains of species of the genus Marmoricola, designated KIS18-7T and JOS5-1T, were isolated from soil samples in Korea. The 16S rRNA gene sequence of strain KIS18-7T showed highest similarities with Marmoricola scoriae Sco-D01T (97.8 %), Marmoricola aequoreus SST-45T (97.6 %) and Marmoricola aurantiacus BC 361T (9...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000184

    authors: Kim SJ,Lim JM,Hamada M,Ahn JH,Weon HY,Suzuki KI,Ahn TY,Kwon SW

    更新日期:2015-06-01 00:00:00

  • Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., isolated from broccoli.

    abstract::A coccal strain isolated from fresh broccoli was initially identified as Enterococcus saccharolyticus; however, molecular identification and phenotypic traits did not support this identification. DNA-DNA hybridization with the type strain of E. saccharolyticus (76.4 % relatedness), DNA G+C content (35.7 mol%), phyloge...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.052761-0

    authors: Chen YS,Lin YH,Pan SF,Ji SH,Chang YC,Yu CR,Liou MS,Wu HC,Otoguro M,Yanagida F,Liao CC,Chiu CM,Huang BQ

    更新日期:2013-12-01 00:00:00

  • Bacteroides xylanisolvens sp. nov., a xylan-degrading bacterium isolated from human faeces.

    abstract::During the course of a study on the xylan-degrading community from the human gut, six xylanolytic, Gram-negative, anaerobic rods were isolated from faecal samples. 16S rRNA gene sequence analysis showed that the isolates were closely related to each other (> or =99 % sequence similarity) and that they belonged to the ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65504-0

    authors: Chassard C,Delmas E,Lawson PA,Bernalier-Donadille A

    更新日期:2008-04-01 00:00:00

  • Kineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment.

    abstract::Two novel actinobacteria, designated YIM 121936T and YIM 121940T, were isolated from alkaline sediment in Yuanjiang, China. The cells of the novel strains were Gram-stain-positive, aerobic, motile, non-spore-forming and coccus-shaped. The two strains both contained meso-diaminopimelic acid as the diagnostic diamino ac...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002380

    authors: Xu FJ,Li QY,Li GD,Chen X,Jiang Y,Jiang CL

    更新日期:2017-11-01 00:00:00

  • Classification of 'Sarraceniospora aurea' Furihata et al. 1989 as Actinocorallia aurea sp. nov.

    abstract::Phylogenetic analysis of 'Sarraceniospora aurea' NBRC 14752 and strain NBRC 15120, based on 16S rRNA gene sequences, revealed that these organisms are related to members of the genus Actinocorallia. These organisms contained glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids and the menaquino...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64008-0

    authors: Tamura T,Hatano K,Suzuki KI

    更新日期:2007-09-01 00:00:00

  • Chryseobacterium gregarium sp. nov., isolated from decaying plant material.

    abstract::In this study, strain P 461/12T, isolated from decaying plant material after mulching a pasture, was shown to represent a novel species of the genus Chryseobacterium by means of a polyphasic approach. The closest phylogenetic neighbours to the novel strain, as determined by 16S rRNA gene sequence analysis, were Chryse...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65544-0

    authors: Behrendt U,Ulrich A,Schumann P

    更新日期:2008-05-01 00:00:00

  • Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water.

    abstract::A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain NAA-13T, was isolated from a freshwater spring located in Kaoshiung, southern Taiwan. This strain was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its class...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65810-0

    authors: Chou JH,Cho NT,Arun AB,Young CC,Chen WM

    更新日期:2008-08-01 00:00:00

  • Dactylosporangium siamense sp. nov., isolated from soil.

    abstract::A novel actinomycete strain, designed MW4-36(T), was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangioph...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.052092-0

    authors: Thawai C,Suriyachadkun C

    更新日期:2013-11-01 00:00:00

  • Spirosoma metallilatum sp. nov., isolated from an automotive air conditioning system.

    abstract::A Gram-stain-negative, aerobic, rod-shaped and yellow-pigmented bacterial strain, designated TX0405T, was isolated from an automotive air conditioning system. Colonies were circular, convex, semi-translucent, smooth and yellow. The strain grew at 20-28°C (optimum, 28°C), at pH 6.0-7.5 (optimum, pH 6.5) and in the pres...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002533

    authors: Kim DU,Lee H,Lee S,Park S,Yoon JH,Ka JO

    更新日期:2018-02-01 00:00:00

  • Francisella guangzhouensis sp. nov., isolated from air-conditioning systems.

    abstract::Four strains (08HL01032(T), 09HG994, 10HP82-6 and 10HL1960) were isolated from water of air-conditioning systems of various cooling towers in Guangzhou city, China. Cells were Gram-stain-negative coccobacilli without flagella, catalase-positive and oxidase-negative, showing no reduction of nitrate, no hydrolysis of ur...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.049916-0

    authors: Qu PH,Chen SY,Scholz HC,Busse HJ,Gu Q,Kämpfer P,Foster JT,Glaeser SP,Chen C,Yang ZC

    更新日期:2013-10-01 00:00:00

  • Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete.

    abstract::A bacterial strain, SL-1T, capable of degrading trichloroethene was isolated from a laboratory enrichment in the Department of Civil and Environmental Engineering, University of Washington, USA. The material in the enrichments was derived from a soil sample from Seattle, WA, USA. Strain SL-1T was capable of using phen...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02488-0

    authors: Lee SB,Strand SE,Stensel HD,Herwig RP

    更新日期:2004-01-01 00:00:00

  • Lactococcus formosensis sp. nov., a lactic acid bacterium isolated from yan-tsai-shin (fermented broccoli stems).

    abstract::A coccal-shaped organism, designated 516(T), was isolated from yan-tsai-shin (fermented broccoli stems), a traditional fermented food in Taiwan. 16S rRNA gene sequencing results showed that strain 516(T) had 98.9 % sequence similarity to that of the type strain Lactococcus garvieae NBRC 100934(T). Comparison of three ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.052811-0

    authors: Chen YS,Otoguro M,Lin YH,Pan SF,Ji SH,Yu CR,Liou MS,Chang YC,Wu HC,Yanagida F

    更新日期:2014-01-01 00:00:00