Abstract:
:Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (Mars Odyssey Orbiter) and assembly-facility surfaces at the Jet Propulsion Laboratory in California and the Kennedy Space Center in Florida. Phylogenetic analysis of 16S rRNA gene sequences has placed these novel isolates within the genus Bacillus, the greatest sequence similarity (99.9 %) being found with Bacillus pumilus. However, these isolates share a mere 91.2 % gyrB sequence similarity with Bacillus pumilus, rendering their 16S rRNA gene-derived relatedness suspect. Furthermore, DNA-DNA hybridization showed only 54-66 % DNA relatedness between the novel isolates and strains of B. pumilus. rep-PCR fingerprinting and previously reported matrix-assisted laser desorption/ionization time-of-flight mass spectrometry protein profiling clearly distinguished these isolates from B. pumilus. Phenotypic analyses also showed some differentiation between the two genotypic groups, although the fatty acid compositions were almost identical. The polyphasic taxonomic studies revealed distinct clustering of the tested strains into two distinct species. On the basis of phenotypic characteristics and the results of phylogenetic analyses of 16S rRNA and gyrB gene sequences, repetitive element primer-PCR fingerprinting and DNA-DNA hybridization, the 13 isolates represent a novel species of the genus Bacillus, for which the name Bacillus safensis sp. nov. is proposed. The type strain is FO-36b(T) (=ATCC BAA-1126(T)=NBRC 100820(T)).
journal_name
Int J Syst Evol Microbiolauthors
Satomi M,La Duc MT,Venkateswaran Kdoi
10.1099/ijs.0.64189-0subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1735-1740issue
Pt 8eissn
1466-5026issn
1466-5034journal_volume
56pub_type
杂志文章abstract::Two anaerobic, pigmented, non-spore-forming, Gram-stain-negative, rod-shaped strains isolated from the human oral cavity, OMA31(T) and OMA130, were characterized by determining their phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 1...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.012831-0
更新日期:2010-03-01 00:00:00
abstract::Based upon the results of another publication [P. Schumann et al. (2000). Int J Syst Evol Microbiol 50, 1421-1424) it is concluded that the culture Pelczaria aurantia ATCC 49321T (= DSM 12801T) currently being distributed does not conform to the description of the type strain of Pelczaria aurantia (Poston 1994) and th...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-4-1695
更新日期:2000-07-01 00:00:00
abstract::Two strictly anaerobic, crescent-shaped bacterial strains, designated WGT and Ycb08, were isolated from a cellulose-degrading mixed culture enriched from yak rumen contents. The strains were Gram-negative, non-spore-forming and motile, with four to six flagella situated at the centre of the concave side of the cell. T...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007641-0
更新日期:2009-08-01 00:00:00
abstract::Two aerobic, Gram-negative, non-motile, non-sporulating, yellow-pigmented bacteria, strains TH92(T) and TH91, were isolated from a hot spring located in Laibin, Guangxi, in the south-eastern geothermal area of China. The isolates grew at 40-77 degrees C (optimally at 70 degrees C) and at pH 6.0-9.5 (optimally at pH 7....
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007690-0
更新日期:2010-04-01 00:00:00
abstract::A mesophilic, chemolithoautotrophic, neutrophilic and aerobic ammonia-oxidizing archaeon, designated strain MY1T, was isolated from agricultural soil. Microscopic observation revealed short, rod-shaped cells with a diameter of 0.3-0.5 µm and length of 0.6-1.0 µm. The isolate had no flagella and pili, and possessed no ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002926
更新日期:2018-10-01 00:00:00
abstract::In order to develop a detection method for the rice pathogens Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli, the phylogeny of six plant-pathogenic Burkholderia species was analysed using the combined nucleotide sequences of gyrB and rpoD. B. plantarii, B. glumae and B. gladioli formed tight mon...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64184-0
更新日期:2006-05-01 00:00:00
abstract::Five brightly red-pigmented, motile, rod-shaped, extremely halophilic bacteria were isolated from saltern crystallizer ponds in Alicante (two strains) and Mallorca (three strains), Spain. They grew optimally at salt concentrations between 20 and 30% and did not grow below 15% salts. Thus, these isolates are among the ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-52-2-485
更新日期:2002-03-01 00:00:00
abstract::A yellow-pigmented, rod-shaped, Gram-staining-negative, aerobic bacterial strain, designated CJ7(T), was isolated from a freshwater lake at Chung-Ang University in Anseong, South Korea. Results from 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically affiliated with members of the genus Flav...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007955-0
更新日期:2009-07-01 00:00:00
abstract::A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain 280(T)) isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus Paenochrobactrum group with a 16S rRNA gene sequence similarity of 98...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.061101-0
更新日期:2014-05-01 00:00:00
abstract::Two strains of strictly aerobic, moderately halophilic Gram-positive rods were isolated from fermented shrimp paste ('ka-pi') produced in Thailand. They produced a red pigment and grew optimally in the presence of 5-30 % NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64315-0
更新日期:2007-02-01 00:00:00
abstract::The taxonomic position of an endophytic actinomycete, strain GKU 157T, isolated from the roots of a jambolan plum tree (Syzygium cumini L. Skeels) collected at Khao Khitchakut National Park, Chantaburi province, Thailand, was determined using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed th...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000203
更新日期:2015-06-01 00:00:00
abstract::A curved-rod-shaped bacterium was isolated from a marine (100 m depth) water sample collected from Bay of Bengal, Visakhapatnam, India. Strain NIO-S14T, was Gram-stain-negative, motile and pale-yellow. NIO-S14T was able to grow aerobically and anaerobically and could utilize a number of organic substrates. Major fatty...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003059
更新日期:2019-01-01 00:00:00
abstract::Two bacterial isolates from Kuwaiti soil contaminated by crude oil were analysed by using a polyphasic taxonomic approach. The isolates, designated OFN N11 and OFN N12(T), were shown to have molecular, chemical and morphological properties typical of members of the genus Nocardia. Based on a multigenic approach that i...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64815-0
更新日期:2007-07-01 00:00:00
abstract::Representatives of a new cyanobacterial genus, Toxopsis Lamprinou & Pantazidou gen. nov., were found in fresh material from Cave 'Francthi' (Peloponnese, Greece) and isolated in cultures. Ecological data relating to the environmental parameters of the sampling sites are provided, such as the photosynthetically active ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.038679-0
更新日期:2012-12-01 00:00:00
abstract::A rod-shaped, Gram-staining-negative, strictly aerobic, non-motile bacterium with no flexirubin-type pigment, designated as W201ET, was isolated from an intertidal sandy beach in Antarctica. The organism formed faintly yellow, round colonies on marine agar 2216E. The strain required sea salts for growth and grew optim...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002586
更新日期:2018-03-01 00:00:00
abstract::Four unknown strains belonging to the genus Arthrobacter were isolated from plateau wildlife on the Qinghai-Tibet Plateau of PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates were separated into two clusters. Cluster I (strains 785T and 208) had the greatest 16S rRNA gene s...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004414
更新日期:2020-10-01 00:00:00
abstract::An organism commonly referred to as 'megabacterium' colonizes the gastric isthmus of many species of birds. It is weakly gram-positive and periodic acid-Schiff-positive and stains with silver stains. Previous studies have shown that it has a nucleus and a cell wall similar to those seen in fungi. Calcofluor white M2R ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.02514-0
更新日期:2003-07-01 00:00:00
abstract::A haloarchaeal strain (ESP3B_9T) was isolated from the salt pan of Sambhar salt lake, Rajasthan, India. Cells were coccoid, non-motile, Gram-stain-negative and formed reddish-pink pigmented colonies. The strain was aerobic, able to grow at 35-55 °C (optimum, 40 °C), in 20-35 % (25 %) NaCl and pH 8-10 (pH 9). Mg2+ not ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003986
更新日期:2020-03-01 00:00:00
abstract::A Gram-negative, rod-shaped, moderately halophilic bacterium, designated strain YIM 91118(T), motile with a single polar flagellum, was isolated from Xinjiang province in north-west China and subjected to a polyphasic taxonomic study. Strain YIM 91118(T) grew optimally at 37 degrees C, pH 7.0-8.0 and 10% NaCl (w/v). I...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65519-0
更新日期:2008-09-01 00:00:00
abstract::Among a group of marine isolates that were found to be positive for genes of the bacterial photosynthetic reaction centre, a strain was selected for characterization that was phylogenetically close to the genus Roseovarius. The strain, designated DFL-24T, originated from a culture of Alexandrium ostenfeldii (dinoflage...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63832-0
更新日期:2005-11-01 00:00:00
abstract::A dark yellow-coloured bacterium, JS13-8(T), was isolated from a soil sample from Jeju Island, Republic of Korea. The cells were aerobic, Gram-negative, non-motile, short rods (0.5-0.7 x 0.8-1.4 microm). Growth occurred at 15-35 degrees C (optimally at 30 degrees C), at pH 5.0-8.0 (optimally at pH 6.0-7.0) and at 0-1 ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65304-0
更新日期:2008-02-01 00:00:00
abstract::Two novel acidothermophilic archaea, strains Ric-A(T) and Ric-F, were isolated from muddy water samples of a sulfuric hot spring located in Tengchong County, Yunnan Province, PR China. The strains were aerobic and facultatively chemolithoautotrophic. Both strains could oxidize S(0) and K2S4O6 for autotrophic growth, a...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.070870-0
更新日期:2015-02-01 00:00:00
abstract::Two bacterial isolates from the surface water of a haloalkaline lake on the Mongolia Plateau, strains MIM18(T) and MIM19, were characterized; their morphological, physiological and chemotaxonomic characters, and phylogenetic position based on 16S rRNA gene sequences, were determined. The two strains were alkaliphilic,...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.031286-0
更新日期:2012-03-01 00:00:00
abstract::The halophilic archaeal strain, YC82(T), was isolated from Yuncheng salt lake in Shanxi, PR China. Cells from strain YC82(T) were Gram-stain negative, pleomorphic rods, which lysed in distilled water and formed light-red colonies on solid media. Strain YC82(T) grew at 25-50 °C (optimum 37 °C), in 1.4-4.8 M NaCl (optim...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000038
更新日期:2015-03-01 00:00:00
abstract::A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5T, was isolated from rhizosphere soil of Suaeda salsa, in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed th...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004255
更新日期:2020-06-01 00:00:00
abstract::Strain DS-1T is a small (0.8 microm in length and 0.2 microm in diameter) heterotrophic bacterium able to omega-oxygenate the commercial surfactant linear alkylbenzenesulfonate (LAS) and shorten the side chain by beta-oxidation to yield sulfophenylcarboxylates. The morphotype is widespread in cultures able to utilize ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.03020-0
更新日期:2004-09-01 00:00:00
abstract::Strain CBA3207T, a novel Gram-stain-negative, aerobic and rod-shaped bacterium, was isolated from the seashore sand of Jeju island in South Korea. Strain CBA3207T grew optimally at 25-30 °C and pH 7.0-7.5 with 3.0-4.0 % (w/v) NaCl. It was catalase-positive, oxidase-negative, and hydrolysed starch, gelatin, and Tweens ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001645
更新日期:2017-02-01 00:00:00
abstract::A novel siderophore-producing actinomycete, designated PL19T, was isolated from the Scots-pine needle-like leaves collected from TNAU campus, Coimbatore, India. The isolate was chemoorganotrophic in nutrition and able to grow at 30 °C, and the optimum pH and NaCl facilitated the growth pH 6-11 and 0-8 % (w/v), respect...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001336
更新日期:2016-10-01 00:00:00
abstract::A novel Gram-stain-negative, rod-shaped, ivory-white, facultatively anaerobic and catalase-positive bacterium, designated H1304T, was isolated from the gut of sea catfish from Coast of Weihai, China. Optimal growth occurred at 30-33 °C (range, 4-37 °C) and pH 7.0-7.5 (range, pH 6.5-9.0) with 2.0-3.0 % (w/v) NaCl (rang...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003146
更新日期:2019-01-01 00:00:00
abstract::A Gram-staining-positive, aerobic and non-motile strain, 0704P10-1(T), was isolated from hydrothermal sediment of the Indian Ocean. Phylogenetic, phenotypic and chemotaxonomic data for the organism supported that it belonged to the genus Janibacter. Strain 0704P10-1(T) showed 97.2-98.7% 16S rRNA gene sequence similari...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.059527-0
更新日期:2014-07-01 00:00:00