当前位置： SCI文献检索 > EXPERIMENTAL HEMATOLOGY期刊下所有文献 > Role of STAT3 and GATA-1 interactions in gamma-globin gene expression.

Role of STAT3 and GATA-1 interactions in gamma-globin gene expression.

Abstract：

OBJECTIVE:We previously demonstrated a silencing role for signal transducers and activators of transcription 3 (STAT3) in gamma-globin gene regulation in primary erythroid cells. Recently, GATA-1, a key transcription factor involved in hematopoietic cell development, was shown to directly inhibit STAT3 activity in vivo. Therefore, we completed studies to determine if interactions between these two factors influence gamma-globin gene expression. MATERIALS AND METHODS:Chromatin immunoprecipitation assay was used to ascertain in vivo protein binding at the gamma-globin 5' untranslated region (5'UTR); protein-protein interactions were examined by coimmunoprecipitation analysis. In vitro protein-DNA binding were completed using surface plasmon resonance and electrophoretic mobility shift assay. Activity of a luciferase gamma-globin promoter reporter and levels of gamma-globin messenger RNA and fetal hemoglobin in stable K562 cell lines overexpressing STAT3 and GATA-1, were used to determine the influence of the STAT3/GATA-1 interaction on gamma-globin gene expression. RESULTS:We observed interaction between STAT3 and GATA-1 in K562 and mouse erythroleukemia cells in vivo at the gamma-globin 5'UTR by chromatin immunoprecipitation assay. Electrophoretic mobility shift assay performed with a 41-base pair gamma-globin DNA probe (gamma41) demonstrated the presence of STAT3 and GATA-1 proteins in complexes assembled at the gamma-globin 5'UTR. A consensus STAT3 DNA probe inhibited GATA-1-binding in a concentration-dependent manner, and the converse was also true. Enforced STAT3 expression augmented its binding at the gamma-globin 5'UTR in vivo and silenced gamma-promoter-driven luciferase activity. Stable enforced STAT3 expression in K562 cells reduced endogenous gamma-globin messenger RNA level. This effect was reversed by GATA-1. CONCLUSION:These data provide evidence that GATA-1 can reverse STAT3-mediated gamma-globin gene silencing in erythroid cells.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Yao X,Kodeboyina S,Liu L,Dzandu J,Sangerman J,Ofori-Acquah SF,Pace BS

doi

10.1016/j.exphem.2009.05.004

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

889-900

issue

8

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(09)00160-X

journal_volume

37

pub_type

杂志文章

相关文献

EXPERIMENTAL HEMATOLOGY文献大全
  • Purification of human urine colony-stimulating factor by affinity chromatography.

    abstract：:Antisera from rabbits immunized with murine macrophage colony-stimulating factor (CSF-1) were evaluated for cross-reactivity with human urine CSF-1. One cross-reactive antiserum was used to purify CSF from human urine. The IgG fractions from normal rabbit serum and the anti-CSF serum were bound to cyanogen bromide-act...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Waheed A,Shadduck RK

    更新日期：1989-01-01 00:00:00

  • T-cell P/E-selectin ligand alpha(1,3)fucosylation is not required for graft-vs-host disease induction.

    abstract：OBJECTIVE:Recognition of E- and P-selectins on vascular endothelium by their leukocyte glycoprotein counterreceptor P-selectin glycoprotein ligand-1 (PSGL-1) initiates and sustains leukocyte rolling, culminating in extravasation of lymphocytes from blood into organs. PSGL-1 is rendered functional by terminal glycosylat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2005.08.003

    authors： Eom HS,Rubio MT,Means TK,Luster AD,Sykes M

    更新日期：2005-12-01 00:00:00

  • Clonal proliferation in vitro of individual murine and human hemopoietic cells after fluorescence-activated cell sorting.

    abstract：:A modification of the fluorescence-activated cell sorter (FACS) was used to rapidly and reliably study the clonal proliferation of single hemopoietic cells. Murine FDC-P1 and human cord blood progenitor cells were examined for their ability to proliferate from single cells in 96-well microtiter plates containing agar ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Kannourakis G,Johnson GR,Battye F

    更新日期：1988-06-01 00:00:00

  • V617F JAK-2 mutation in patients with essential thrombocythemia: relation to platelet, granulocyte, and plasma hemostatic and inflammatory molecules.

    abstract：OBJECTIVE:This article evaluates patients with essential thrombocythemia (ET) to determine whether the V617F mutation in the JAK2 gene affects platelet hemostatic and adhesive molecules, platelet-polymorphonuclear leukocyte (PMN) interactions, and PMN-activation characteristics, as well as plasma hypercoagulation marke...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2007.01.053

    authors： Falanga A,Marchetti M,Vignoli A,Balducci D,Russo L,Guerini V,Barbui T

    更新日期：2007-05-01 00:00:00

  • Analysis of leukocyte antigens detected by immunoenzymatic staining on human bone marrow colonies.

    abstract：:Immunoenzymatic (IE) staining for antigen detection on intact human bone marrow (BM) colonies grown in agar layers is described. The reliability of this method allowed a study of antigenic changes during myelomonocytic differentiation. Phenotypic analysis was carried out at different times using the following monoclon...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Villa S,Delia D

    更新日期：1988-06-01 00:00:00

  • Hematologic effects of recombinant human interleukin-6 in dogs exposed to a total-body radiation dose of 2.4 Gy.

    abstract：:The hematologic effects of recombinant human interleukin-6 (rhIL-6) were studied in dogs exposed to a total-body irradiation (TBI) of 2.4 Gy. IL-6 was administered over a period of 14 days at a daily dose of 18 micrograms/kg by single subcutaneous injection. Treatment was started 1 day after TBI. The data obtained for...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Selig C,Kreja L,Müller H,Seifried E,Nothdurft W

    更新日期：1994-07-01 00:00:00

  • Busulfan-conditioned bone marrow transplantation results in high-level allogeneic chimerism in mice made tolerant by in utero hematopoietic cell transplantation.

    abstract：OBJECTIVE:In utero hematopoietic cell transplantation (IUHCT) is a non-ablative approach that achieves mixed allogeneic chimerism and donor-specific tolerance. However, clinical application of IUHCT has been limited by minimal engraftment. We have previously demonstrated in the murine model that low-level allogeneic ch...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2005.11.011

    authors： Ashizuka S,Peranteau WH,Hayashi S,Flake AW

    更新日期：2006-03-01 00:00:00

  • Enzymatic and functional correction along with long-term enzyme secretion from transduced bone marrow hematopoietic stem/progenitor and stromal cells derived from patients with Fabry disease.

    abstract：:Fabry disease is a lysosomal storage disorder that is due to a deficiency in alpha-galactosidase A (alpha-gal A). Previously we have shown that a recombinant retrovirus synthesized for the transfer of the human alpha-gal A coding sequence was able to engineer enzymatic correction of the hydrolase deficiency in fibrobl...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(99)00050-8

    authors： Takenaka T,Hendrickson CS,Tworek DM,Tudor M,Schiffmann R,Brady RO,Medin JA

    更新日期：1999-07-01 00:00:00

  • CBP/Catenin antagonists: Targeting LSCs' Achilles heel.

    abstract：:Cancer stem cells (CSCs), including leukemia stem cells (LSCs), exhibit self-renewal capacity and differentiation potential and have the capacity to maintain or renew and propagate a tumor/leukemia. The initial isolation of CSCs/LSCs was in adult myelogenous leukemia, although more recently, the existence of CSCs in a...

    journal_title：Experimental hematology

    pub_type： 杂志文章,评审

    doi：10.1016/j.exphem.2017.04.010

    authors： Kim YM,Gang EJ,Kahn M

    更新日期：2017-08-01 00:00:00

  • Depletion of donor-reactive cells as a new concept for improvement of mismatched bone marrow engraftment using reduced-intensity conditioning.

    abstract：OBJECTIVE:New nonmyeloablative strategies to improve acceptance of mismatched bone marrow (BM) may compensate for the inadequate supply of compatible grafts. Recently we proposed to facilitate engraftment of mismatched BM by selective depletion of activated donor-reactive host cells with cyclophosphamide (CY). Here we ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2004.07.017

    authors： Prigozhina TB,Elkin G,Khitrin S,Slavin S

    更新日期：2004-11-01 00:00:00

  • Identification of E74-like factor 1 (ELF1) as a transcriptional regulator of the Hox cofactor MEIS1.

    abstract：OBJECTIVE:Myeloid ectropic viral integration site 1 (MEIS1) is a Hox cofactor known for its role in development and is strongly linked to normal and leukemic hematopoiesis. Although previous studies have focused on identifying protein partners of MEIS1 and its transcriptionally regulated targets, little is known about ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2010.06.006

    authors： Xiang P,Lo C,Argiropoulos B,Lai CB,Rouhi A,Imren S,Jiang X,Mager D,Humphries RK

    更新日期：2010-09-01 00:00:00

  • Immunologic recovery after hematopoietic cell transplantation with nonmyeloablative conditioning.

    abstract：OBJECTIVE:We studied immune reconstitution in 51 recipients of HLA-identical hematopoietic cellular transplant (HCT) after nonmyeloablative conditioning compared to a reference group of 67 recipients after myeloablative conditioning. METHODS:Nonmyeloablative conditioning consisted of 2 Gy total-body irradiation+/-flud...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(03)00201-7

    authors： Maris M,Boeckh M,Storer B,Dawson M,White K,Keng M,Sandmaier B,Maloney D,Storb R,Storek J

    更新日期：2003-10-01 00:00:00

  • Separation of CFU-S from primitive cells responsible for reconstitution of the bone marrow hemopoietic stem cell compartment following irradiation: evidence for a pre-CFU-S cell.

    abstract：:We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is show...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Ploemacher RE,Brons RH

    更新日期：1989-03-01 00:00:00

  • CD133-enriched CD34(-) (CD33/CD38/CD71)(-) cord blood cells acquire CD34 prior to cell division and hematopoietic activity is exclusively associated with CD34 expression.

    abstract：:We compared the cell division behavior of CD34(-) and CD34(+) (CD33/CD38/CD71)-negative (Lin(-)) CD133(+) cord blood cells stimulated with the cytokines Flt3-ligand, stem cell factor, and thrombopoietin. Within a 4-day time frame, Lin(-)CD34(-) CD133(+) (CD34(-)) cells underwent more cell divisions in serum-free cultu...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2007.05.016

    authors： Götze KS,Schiemann M,Marz S,Jacobs VR,Debus G,Peschel C,Oostendorp RA

    更新日期：2007-09-01 00:00:00

  • Responses of the murine myeloid cell line FDC-P1 to soluble and membrane-bound forms of steel factor (SLF).

    abstract：:Cells of the murine interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) factor-dependent line, FDC-P1, express the tyrosine kinase receptor, c-kit. The ligand for c-kit, steel factor (SLF), encoded by the steel (Sl) locus, is produced as both membrane-bound and soluble forms by fibroblast...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Caruana G,Ashman LK,Fujita J,Gonda TJ

    更新日期：1993-06-01 00:00:00

  • The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI.

    abstract：:The high affinity Fc gamma receptor, Fc gamma RI, is unique among the three classes of macrophage Fc gamma receptors not only in its affinity for IgG, but also in the structure of its cytoplasmic domain. Fc gamma RIIA and the gamma subunit of Fc gamma RIIIA have tyrosine-containing motifs within their cytoplasmic doma...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Indik ZK,Hunter S,Huang MM,Pan XQ,Chien P,Kelly C,Levinson AI,Kimberly RP,Schreiber AD

    更新日期：1994-07-01 00:00:00

  • A multifactorial analysis of umbilical cord blood, adult bone marrow and mobilized peripheral blood progenitors using the improved ML-IC assay.

    abstract：OBJECTIVE:Assays that can evaluate the potential of individual human hematopoietic stem cells (HSC) are still lacking. We previously developed the myeloid-lymphoid initiating cell (ML-IC) assay that enumerates single CD34(+) cells that generate long-term culture-initiating (LTC-IC) and NK-initiating (NK-IC) daughter ce...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2004.10.016

    authors： Theunissen K,Verfaillie CM

    更新日期：2005-02-01 00:00:00

  • Marrow repopulating cells, but not CFU-S, establish long-term in vitro hemopoiesis on a marrow-derived stromal layer.

    abstract：:We have studied the ability of subpopulations of hemopoietic stem cells, obtained from murine bone marrow using elutriation and multiparameter sorting, to establish and maintain hemopoiesis following their deposition on irradiated stromal layers of long-term bone marrow cultures. Two fractions were obtained that diffe...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： van der Sluijs JP,de Jong JP,Brons NH,Ploemacher RE

    更新日期：1990-09-01 00:00:00

  • Destruction of hematopoietic microenvironment by cytotoxic T cells.

    abstract：:Coculture of cytotoxic T cells (STIL-3 C5) derived from L8313 leukemic mice with hematopoietic supportive stromal cells (MS-5) resulted in the detachment of MS-5 cells from the culture dish, whereas helper T cells (STIL-3 DF) did not induce this detachment. The response of bone marrow (BM) adherent cells to the same t...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Takashita E,Sugimoto K,Adachi Y,Aihara Y,Inoue H,Jiang H,Katakai T,Mori KJ

    更新日期：1997-09-01 00:00:00

  • Are stem cells exposed to ionizing radiation in vivo as effective as nonirradiated transfused stem cells in restoring hematopoiesis?

    abstract：:Are the stem cells that survive graded doses of ionizing radiation as effective in restoring hematopoiesis in irradiated mice as transfused nonirradiated stem cells? This question was addressed by determining animal dose mortality and 10-day colony-forming units (CFU-S) survival curves and then replotting the percent ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Cronkite EP,Inoue T,Hirabayashi Y,Bullis J

    更新日期：1993-06-01 00:00:00

  • Radioresistant murine marrow stromal cells: a morphologic and functional characterization.

    abstract：:Two types of murine marrow adherent cells derived from Dexter cultures have been characterized. Exposure of C57B1/6J, ICR, or BDF1 mice to 1000 R x-ray 24 h prior to killing and establishment of liquid marrow cultures resulted in the growth of two types of adherent cells. A macrophage-like cell was phagocytic, nonspec...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Song ZX,Quesenberry PJ

    更新日期：1984-08-01 00:00:00

  • A murine model for genetic manipulation of the T cell compartment.

    abstract：:The expression of exogenous genes in long-lived primary T cells is potentially beneficial for the treatment of various diseases including cancer, AIDS, genetic defects of the lymphoid compartment, and systemic enzyme deficiencies such as hemophilia. One approach for genetic modification of T cells is to introduce ther...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Gu J,Kuo ML,Rivera A,Sutkowski N,Ron Y,Dougherty JP

    更新日期：1996-10-01 00:00:00

  • Comparative analysis of naïve and memory CD4+ and CD8+ T-cell subsets in bone marrow and G-CSF-mobilized peripheral blood stem cell allografts: impact of donor characteristics.

    abstract：OBJECTIVE:Donor T cells expressing lymph node homing receptors are the foremost initiators of acute graft-vs-host disease (aGVHD), and a high proportion of CD4(+)CCR7(+) T cells in human leukocyte antigen-matched allografts has been shown to confer a high risk of aGVHD without interfering in other outcomes. METHODS:Na...

    journal_title：Experimental hematology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.exphem.2007.03.006

    authors： Yakoub-Agha I,Saule P,Depil S,Grutzmacher C,Boulanger F,Magro L,Jouet JP,Dessaint JP,Labalette M

    更新日期：2007-06-01 00:00:00

  • Studies on the hyperthermic sensitivity of the murine hematopoietic stem cell compartment. I. Heat effects on clonogenic stem cells and progenitors.

    abstract：:The heat sensitivity at 42 degrees, 43 degrees and 44 degrees C of various hematopoietic subsets in murine bone marrow (MRA, CFU-S-12, CFU-S-8, CFU-GM, BFU-E and CFU-E) was investigated in order to determine whether there is a relationship between heat sensitivity and the position of cells within the stem cell hierarc...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Wierenga PK,Konings AW

    更新日期：1993-05-01 00:00:00

  • Blast colony-forming cells and precursors of colony-forming cells detectable in long-term marrow culture express the same phenotype (CD33- CD34+).

    abstract：:We have previously shown that CD33- CD34+ human marrow cells are capable of giving rise to colony-forming cells (CFC) in long-term marrow culture (LTMC) but are mainly depleted of progenitors that directly form colonies in semi-solid media. In contrast, the CD33+ CD34+ population contains most of the CFC but not the p...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Bernstein ID,Leary AG,Andrews RG,Ogawa M

    更新日期：1991-08-01 00:00:00

  • Lymphoid cell proliferation in non-lymphoid organs during graft-versus-host reactions (GVHR): I. Lack of correlation between mortality and grafted cell proliferation.

    abstract：:The course of development of graft-versus-host reactions (GVHR) following lethal irradiation was compared in two mouse donor-host combinations disparate at non H-2 loci alone or associated to H-2 complex. The pathogenesis of these reactions was very different (mortality rates faster in the second situation for example...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Spach C,Motta R

    更新日期：1981-05-01 00:00:00

  • Heterozygous kit mutants with little or no apparent anemia exhibit large defects in overall hematopoietic stem cell function.

    abstract：OBJECTIVE:The evolutionarily conserved Kit receptor is vital for function of hematopoietic stem cells (HSC). Kit(W-41) (W-41) and Kit(W-42) (W-42) are single residue changes in the KIT intracellular phosphotransferase domain, while Kit(W-v) (W-v) is a single residue change in the ATP binding domain. This study tests ho...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.10.001

    authors： Sharma Y,Astle CM,Harrison DE

    更新日期：2007-02-01 00:00:00

  • The leukopoietic cytokine granulocyte colony-stimulating factor increases blood flow to rat bone marrow.

    abstract：:The current knowledge concerning the blood supply to hematopoietic bone marrow during increased marrow metabolism is scanty. We have previously shown that an accelerated erythropoiesis in the awake rat is accompanied by a rapid increase in perfusion of the tibial marrow and its bony encasement. We have now measured bl...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Iversen PO,Nicolaysen G,Benestad HB

    更新日期：1993-02-01 00:00:00

  • Thyroid hormones enhance hypoxia-induced erythropoietin production in vitro.

    abstract：:Effects of thyroid hormones on the production of erythropoietin (Epo) were investigated in isolated perfused rat kidneys and in the human hepatoma cell line, HepG2. Epo protein was measured by radioimmunoassay. L-triiodothyronine and L-thyroxine stimulated hypoxia-induced Epo formation both in the kidney and in HepG2 ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Fandrey J,Pagel H,Frede S,Wolff M,Jelkmann W

    更新日期：1994-03-01 00:00:00

  • Effects of in vivo administration of stem cell factor on thrombopoiesis in normal and immunodeficient mice.

    abstract：:Thrombocytopenia is an important clinical problem for many acquired immunodeficiency syndrome (AIDS) patients. Recently, the utility of recombinant cytokines in alleviating the hematopoietic complications of AIDS and AIDS therapy has been evaluated. The newly cloned cytokine stem cell factor (SCF) has been demonstrate...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Chow FP,Zsebo K,Hamburger AW

    更新日期：1993-08-01 00:00:00