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Calcium ions regulate K⁺ uptake into brain mitochondria: the evidence for a novel potassium channel.

Abstract：

:The mitochondrial response to changes of cytosolic calcium concentration has a strong impact on neuronal cell metabolism and viability. We observed that Ca(2+) additions to isolated rat brain mitochondria induced in potassium ion containing media a mitochondrial membrane potential depolarization and an accompanying increase of mitochondrial respiration. These Ca(2+) effects can be blocked by iberiotoxin and charybdotoxin, well known inhibitors of large conductance potassium channel (BK(Ca) channel). Furthermore, NS1619 - a BK(Ca) channel opener - induced potassium ion-specific effects on brain mitochondria similar to those induced by Ca(2+). These findings suggest the presence of a calcium-activated, large conductance potassium channel (sensitive to charybdotoxin and NS1619), which was confirmed by reconstitution of the mitochondrial inner membrane into planar lipid bilayers. The conductance of the reconstituted channel was 265 pS under gradient (50/450 mM KCl) conditions. Its reversal potential was equal to 50 mV, which proved that the examined channel was cation-selective. We also observed immunoreactivity of anti-beta(4) subunit (of the BK(Ca) channel) antibodies with ~26 kDa proteins of rat brain mitochondria. Immunohistochemical analysis confirmed the predominant occurrence of beta(4) subunit in neuronal mitochondria. We hypothesize that the mitochondrial BK(Ca) channel represents a calcium sensor, which can contribute to neuronal signal transduction and survival.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Skalska J,Bednarczyk P,Piwońska M,Kulawiak B,Wilczynski G,Dołowy K,Kudin AP,Kunz WS,Szewczyk A

doi

10.3390/ijms10031104

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

1104-20

issue

3

issn

1422-0067

journal_volume

10

pub_type

杂志文章

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