Molecular composition and functional properties of f-channels in murine embryonic stem cell-derived pacemaker cells.

abstract：:Glutamic dehydrogenase purified from rat heart mitochondria has been characterized with regard to its substrate kinetics and the influence of nucleotides and potassium phosphate on its kinetic properties. The enzyme had characteristics similar to liver mitochondrial glutamic dehydrogenase. These included several doubl...

journal_title：Journal of molecular and cellular cardiology

authors： McDaniel HG,Jenkins R,Yeh M,Razzaque A

更新日期：1984-04-01 00:00:00

abstract：:Gain- and loss-of-function strategies using transgenic over-expression and targeted ablation of candidate genes in in the mouse have provided important mechanistic insights into cardiovascular development, physiology and disease. An essential, but challenging step is the functional analysis of the resultant phenotype....

journal_title：Journal of molecular and cellular cardiology

authors： Hoit BD

更新日期：2001-01-01 00:00:00

abstract：:An efficient cardiac gene transfer technique in murine models would greatly facilitate the elucidation of the pathophysiology of cardiomyopathies and the development of genetic therapies. Direct myocardial injection or catheter-based intracoronary infusion is not easily achievable in mice and resultant transgene expre...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang JC,Woo YJ,Chen JA,Swain JL,Sweeney HL

更新日期：1999-04-01 00:00:00

abstract：:Pathologic cardiac hypertrophy can lead to heart failure, but the mechanisms involved are poorly understood. SERCA2 is critical for normal cardiac calcium handling and function and SERCA2 mRNA and protein levels are reduced by cardiac hypertrophy. We hypothesized that extracellular signal-regulated kinase (ERK) 1/2 ac...

journal_title：Journal of molecular and cellular cardiology

authors： Huang H,Joseph LC,Gurin MI,Thorp EB,Morrow JP

更新日期：2014-10-01 00:00:00

abstract：:Fibrosis, a consequence of tissue repair, can become a final common pathway to organ failure, if progressive. Prevention and regression of organ fibrosis represent targets of considerable interest. The natural fate of fibrosis differs among various tissues being either persistent, progressive or regressive. Cellular a...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang J,Sun Y,Zhang JQ,Ramires FJ,Weber KT

更新日期：1999-05-01 00:00:00

abstract：:Coronary blood flow is tightly adjusted to the oxygen requirements of the myocardium. The underlying control mechanisms keep coronary venous pO(2) at a rather constant level around 20mm Hg under a variety of physiological conditions. Because coronary flow may increase more than 5-fold during exercise without any signs...

journal_title：Journal of molecular and cellular cardiology

authors： Deussen A,Ohanyan V,Jannasch A,Yin L,Chilian W

更新日期：2012-04-01 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2), administered to the isolated rat heart by perfusion and under constant pressure, is protective against ischemia-reperfusion (I-R). Here we have investigated whether FGF-2 cardioprotection: (a) is dependent on flow modulation; (b) is linked to effects on contractility; (c) is mediate...

journal_title：Journal of molecular and cellular cardiology

authors： Padua RR,Merle PL,Doble BW,Yu CH,Zahradka P,Pierce GN,Panagia V,Kardami E

更新日期：1998-12-01 00:00:00

abstract：:Caloric restriction extends longevity and reduces the onset of chronic disease in many animal models. Recently, caloric restriction was shown in humans to be associated with lower blood pressure, decreased systemic inflammation, and improved cardiac diastolic parameters. However, the causation and mechanisms of calori...

journal_title：Journal of molecular and cellular cardiology

authors： Seymour EM,Parikh RV,Singer AA,Bolling SF

更新日期：2006-10-01 00:00:00

abstract：:Changes in the capacities of ATP-synthesizing reactions were analysed in residual non-infarcted myocardium following myocardial infarction. Rats were subjected to left coronary artery ligation (MI; n = 11) or to sham operation (sham; n = 18). Two months later, hearts were excised, rinsed and buffer-perfused isovolumic...

journal_title：Journal of molecular and cellular cardiology

authors： Laser A,Ingwall JS,Tian R,Reis I,Hu K,Gaudron P,Ertl G,Neubauer S

更新日期：1996-07-01 00:00:00

abstract：:Short duration exposure to cellular stresses have been shown to activate p38 mitogen-activated protein kinase (MAPK) in cultured rat ventricular cardiomyocytes and isolated perfused hearts; however, effects of chronic stress on p38 MAPK are not well understood. This study determined whether alterations in the p38 MAPK...

journal_title：Journal of molecular and cellular cardiology

authors： Lemke LE,Bloem LJ,Fouts R,Esterman M,Sandusky G,Vlahos CJ

更新日期：2001-08-01 00:00:00

abstract：:The lack of pathological consequences of cardiac ATP-sensitive potassium channel (K(ATP)) channel gene manipulation is in stark contrast to the effect of similar perturbations in the pancreatic beta-cell. Because the pancreatic and cardiac channel share the same pore-forming subunit (Kir6.2), the different effects of ...

journal_title：Journal of molecular and cellular cardiology

authors： Flagg TP,Remedi MS,Masia R,Gomes J,McLerie M,Lopatin AN,Nichols CG

更新日期：2005-10-01 00:00:00

abstract：:The effects of trimetazidine on membrane potentials and membrane currents of enzymatically isolated guinea-pig ventricular cells were studied with the use of giga-seal suction pipettes for patch clamp. Trimetazidine (3 X 10(-5) M) decreased the action potential duration from 433 +/- 179 ms (mean and S.D., n = 9) to 31...

journal_title：Journal of molecular and cellular cardiology

authors： Kiyosue T,Nakamura S,Arita M

更新日期：1986-12-01 00:00:00

abstract：:Recent evidence has shown that the cardioprotection afforded by the late phase of ischemic preconditioning (PC) is mediated by upregulation of inducible nitric oxide synthase (iNOS). However, the specific cardiac cell type(s) that express(es) iNOS in response to ischemic PC remains unknown. Thus, mice underwent a sequ...

journal_title：Journal of molecular and cellular cardiology

authors： Wang Y,Guo Y,Zhang SX,Wu WJ,Wang J,Bao W,Bolli R

更新日期：2002-01-01 00:00:00

abstract：:The Gq-RhoA-Rho kinase pathway, activated by neurohormonal factors such as angiotensin II (Ang II), has been proposed to be one of the important signaling pathways involved in the progression of left ventricular (LV) hypertrophy to heart failure. We tested the hypothesis that chronic inhibition of Rho kinase prevents ...

journal_title：Journal of molecular and cellular cardiology

authors： Satoh S,Ueda Y,Koyanagi M,Kadokami T,Sugano M,Yoshikawa Y,Makino N

更新日期：2003-01-01 00:00:00

abstract：:Phosphorylation of myosin binding protein C (MyBP-C) was investigated in intraventricular septum samples taken from patients with hypertrophic cardiomyopathy undergoing surgical septal myectomy. These samples were compared with donor heart muscle, as a well-characterised control tissue, and with end-stage failing hear...

journal_title：Journal of molecular and cellular cardiology

authors： Jacques AM,Copeland O,Messer AE,Gallon CE,King K,McKenna WJ,Tsang VT,Marston SB

更新日期：2008-08-01 00:00:00

abstract：AIMS:LCZ696, a novel angiotensin receptor neprilysin inhibitor, is effective in treating heart failure patients. Doxorubicin (DOX) is an effective antitumor medication but the cardiotoxicity limited its clinical use. In this study, we aimed to determine the effect of LCZ696 on DOX-induced cardiomyopathy in mice and in ...

journal_title：Journal of molecular and cellular cardiology

authors： Xia Y,Chen Z,Chen A,Fu M,Dong Z,Hu K,Yang X,Zou Y,Sun A,Qian J,Ge J

更新日期：2017-07-01 00:00:00

abstract：:The mitochondrial calcium uniporter (MCU) relays cytosolic Ca2+ transients to the mitochondria. We examined whether energy metabolism was compromised in hearts from mice with a cardiac-specific deficiency of MCU subjected to an isoproterenol (ISO) challenge. Surprisingly, isolated working hearts from cardiac MCU-defic...

journal_title：Journal of molecular and cellular cardiology

authors： Altamimi TR,Karwi QG,Uddin GM,Fukushima A,Kwong JQ,Molkentin JD,Lopaschuk GD

更新日期：2019-02-01 00:00:00

abstract：:Effects of hypoxia-reoxygenation (H-R) on myocytes isolated from 10 week hypertrophied and sham control rat hearts were studied. Myocyte hypertrophy was indicated by an increase in cell size. Superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) enzyme activities were significantly higher and lipid peroxidatio...

journal_title：Journal of molecular and cellular cardiology

authors： Kirshenbaum LA,Hill M,Singal PK

更新日期：1995-01-01 00:00:00

abstract：:To examine cardiac myosin gene structure and expression in a non-human primate model for human heart development and disease, we have constructed a cDNA library from baboon atrium and used baboon beta-myosin heavy chain (beta-MHC)* cDNA probes to isolate atrial MHC clones. The nucleotide sequence of one such clone, la...

journal_title：Journal of molecular and cellular cardiology

authors： Hixson JE,Henkel RD,Britten ML,Vernier DT,deLemos RA,VandeBerg JL,Walsh RA

更新日期：1989-10-01 00:00:00

abstract：:Chronic pain aggravates cardiovascular injury via incompletely understood mechanisms. While melatonin may participate in the pathophysiological process of chronic pain, its cardiovascular effects under chronic pain states remains unknown. In this study, chronic pain was induced by spared nerve injury model (SNI) for 4...

journal_title：Journal of molecular and cellular cardiology

authors： Yang Z,Li C,Wang Y,Yang J,Yin Y,Liu M,Shi Z,Mu N,Yu L,Ma H

更新日期：2018-12-01 00:00:00

abstract：:Microtubules have been implicated in a number of muscle-specific functions, including sarcomerogenesis and the regulation of heart cell beating rate. Post-translationally modified microtubules (MTs) have been correlated, in other cell types, with MTs of increased stability and possibly distinct functions. This study w...

journal_title：Journal of molecular and cellular cardiology

authors： Webster DR

更新日期：1997-06-01 00:00:00

abstract：:Arterial hypotension can cause cerebral ischemia when the autoregulation of the cerebral circulation is exhausted. We hypothesized that sudden cerebral vasoconstriction induced by moderate hypotensive, but hemodynamically stable, sustained ventricular tachycardias (MHT-VT) further compromises cerebral blood flow (CBF)...

journal_title：Journal of molecular and cellular cardiology

authors： Hagendorff A,Dettmers C,Danos P,Hümmelgen M,Vahlhaus C,Martin C,Heusch G,Lüderitz B

更新日期：1998-10-01 00:00:00

abstract：:Cardiac disease is frequently associated with abnormalities in electrical function that can severely impair cardiac performance with potentially fatal consequences. The available therapeutic options have some efficacy but are far from perfect. The curative potential of gene therapy makes it an attractive approach for ...

journal_title：Journal of molecular and cellular cardiology

authors： Greener I,Donahue JK

更新日期：2011-05-01 00:00:00

abstract：:Quin2 is a fluorescent Ca2+-sensitive indicator which in its acetoxymethyl ester form can be loaded into the cytosolic compartment of cells without any disruption of the plasma membrane. Quin2 has been used with many cell types to measure changes of [Ca2+]i in response to hormones and other stimuli. In this report we ...

journal_title：Journal of molecular and cellular cardiology

authors： Thomas AP,Selak M,Williamson JR

更新日期：1986-05-01 00:00:00

abstract：:Previous work on the rat heart has demonstrated an age-related reduction in catecholamines and a decline in myocardial cell sensitivity to catecholamines in vitro. We used ultrastructural cytochemical techniques to label noradrenergic vesicles of the sympathetic nerve terminals of the rat heart atrium, and addressed t...

journal_title：Journal of molecular and cellular cardiology

authors： McLean MR,Goldberg PB,Roberts J

更新日期：1983-02-01 00:00:00

abstract：:Chemokines are small molecular weight proteins that play important roles in inflammation. Originally described as chemotactic cytokines, chemokines stimulate the influx of leukocytes into specific tissue compartments. These molecules also modulate gene expression in both infiltrating and resident cells to mediate a va...

journal_title：Journal of molecular and cellular cardiology

authors： Tarzami ST,Cheng R,Miao W,Kitsis RN,Berman JW

更新日期：2002-02-01 00:00:00

abstract：:Transgenic mice with cardiac-specific overexpression of G alpha q exhibit a biochemical and physiological phenotype of load-independent cardiac hypertrophy with contractile dysfunction. To elucidate the cellular basis for altered contractility, we measured cellular contraction, Ca(2+)transients, and l -type Ca(2+)chan...

journal_title：Journal of molecular and cellular cardiology

authors： Yatani A,Frank K,Sako H,Kranias EG,Dorn GW 2nd

更新日期：1999-07-01 00:00:00

abstract：:Mammalian cardiomyocytes withdraw from the cell cycle shortly after birth, although it remains unclear how cardiomyocyte cell cycles behave during development. Compared to conventional immunohistochemistry in static observation, time-lapse imaging can reveal comprehensive data in hard-to-understand biological phenomen...

journal_title：Journal of molecular and cellular cardiology

authors： Hashimoto H,Yuasa S,Tabata H,Tohyama S,Hayashiji N,Hattori F,Muraoka N,Egashira T,Okata S,Yae K,Seki T,Nishiyama T,Nakajima K,Sakaue-Sawano A,Miyawaki A,Fukuda K

更新日期：2014-07-01 00:00:00

abstract：:In recently published studies, we show that angiotensin II (AII) generated from an engineered rat angiotensinogen cDNA, and maintained intracellularly, is growth stimulatory for a rat hepatoma cell line. In the present study, we report that co-expression of AII fused to cyan fluorescent protein (ECFP/AII) and angioten...

journal_title：Journal of molecular and cellular cardiology

authors： Cook JL,Re R,Alam J,Hart M,Zhang Z

更新日期：2004-01-01 00:00:00

abstract：:The modulating effects of Ca2+ on single K+ channel currents in canine heart sarcoplasmic reticulum were studied using a planar lipid bilayer technique. The open-state probability and the unitary open-state current both decreased gradually as the Ca2+ concentration was reduced from pCa 3 to pCa 7.5. Each single-channe...

journal_title：Journal of molecular and cellular cardiology

authors： Uehara A,Yasukochi M,Imanaga I

更新日期：1994-02-01 00:00:00