当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Transgenic overexpression of SUR1 in the heart suppresses sarcolemmal K(ATP).

Transgenic overexpression of SUR1 in the heart suppresses sarcolemmal K(ATP).

Abstract：

:The lack of pathological consequences of cardiac ATP-sensitive potassium channel (K(ATP)) channel gene manipulation is in stark contrast to the effect of similar perturbations in the pancreatic beta-cell. Because the pancreatic and cardiac channel share the same pore-forming subunit (Kir6.2), the different effects of genetic manipulation likely reflect, at least in part, the tissue-specific expression of the regulatory subunit (SUR1 in pancreas vs. SUR2A in heart) of the bipartite channel complex. To examine this, we have generated transgenic (TG) mice that overexpress epitope-tagged SUR1 or SUR2A under the transcriptional control of the alpha-myosin heavy chain promoter. Western blot and real time RT-PCR analysis confirm transgene expression in the heart, and variable levels of SUR1 RNA and protein, in 16 viable founder lines. Surprisingly, activation of channels by either pharmacological agents (diazoxide and pinacidil) or metabolic inhibitors (oligomycin and 2-deoxyglucose) reveals a suppression of total K(ATP) conductance in high expressing TG mice. Moreover, K(ATP) channel activity was significantly reduced in excised cardiac patches from TG myocytes that overexpress either SUR1 or SUR2A. Using a recombinant cell system, we show that overexpression of either SUR1 or Kir6.2 suppresses the functional expression of K(ATP) from optimized dimeric SUR1-Kir6.2. Thus, the graded effect of SUR1 expression in the intact heart appears to demonstrate an in vivo requirement for 1:1 expression ratio of Kir6.2 and SURx.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Flagg TP,Remedi MS,Masia R,Gomes J,McLerie M,Lopatin AN,Nichols CG

doi

10.1016/j.yjmcc.2005.06.003

keywords:

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

647-56

issue

4

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(05)00187-2

journal_volume

39

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Zinc improves mitochondrial respiratory function and prevents mitochondrial ROS generation at reperfusion by phosphorylating STAT3 at Ser727.

    abstract：:Serine 727 (Ser727) phosphorylation of STAT3 plays a role in the regulation of mitochondrial respiration. This study aimed to test if zinc could regulate mitochondrial respiration through phosphorylation of STAT3 at Ser727 in the setting of ischemia/reperfusion in the heart. Under normoxic conditions, treatment of iso...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.03.019

    authors： Zhang G,Sheng M,Wang J,Teng T,Sun Y,Yang Q,Xu Z

    更新日期：2018-05-01 00:00:00

  • Protection against lipopolysaccharide-induced myocardial dysfunction in mice by cardiac-specific expression of soluble Fas.

    abstract：:The mechanisms responsible for myocardial dysfunction in the setting of sepsis remain undefined. Fas ligation with its cognate ligand (FasL) induces apoptosis and activates cellular inflammatory responses associated with tissue injury. We determined whether interruption of Fas/FasL interaction by cardiac-specific expr...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.09.016

    authors： Niu J,Azfer A,Kolattukudy PE

    更新日期：2008-01-01 00:00:00

  • miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation.

    abstract：:The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue because it has major implications for the prevention of pathological vascular conditions. Using microRNA array screen, we found the expression levels of 200 unique miRNAs in hyperplasic tissues. Among them, miR-200c expression sub...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.03.011

    authors： Zheng B,Bernier M,Zhang XH,Suzuki T,Nie CQ,Li YH,Zhang Y,Song LL,Shi HJ,Liu Y,Zheng CY,Wen JK

    更新日期：2015-05-01 00:00:00

  • Guanylate cyclase activation by organic nitrates is not mediated via nitrite.

    abstract：:Nitrovasodilators relax vascular smooth muscle by stimulating guanylate cyclase. Ignarro et al. (1981) proposed a mechanistic scheme according to which organic nitrates release nitrite in the presence of thiols. The corresponding nitrous acid would decay leading to nitric oxide, which then would react with another thi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(88)80130-5

    authors： Romanin C,Kukovetz WR

    更新日期：1988-05-01 00:00:00

  • Influence of noradrenaline and temperature on the isolated perfused rat heart.

    abstract：:Myocardial protection during open heart surgery is achieved by general cooling and cold cardioplegia. However sympathetic activation during the operation causes raised circulating catecholamine levels that might negatively influence the myocardial metabolism. Moreover, local liberation of catecholamines, in "poorly pe...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(83)90276-6

    authors： Berggren H,Ekroth R,Hjalmarson A,Holm S,William-Olsson G,Waldenström A

    更新日期：1983-04-01 00:00:00

  • Heterotypic docking compatibility of human connexin37 with other vascular connexins.

    abstract：:Human vascular connexins (Cx37, Cx40, Cx43, and Cx45) can form various types of gap junction channels to synchronize vasodilation/constriction to control local circulation. Most of our knowledge on heterotypic gap junctions of these vascular connexins was from studies on rodent connexins. In human vasculature, the sam...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.12.013

    authors： Kim NK,Santos-Miranda A,Chen H,Aoyama H,Bai D

    更新日期：2019-02-01 00:00:00

  • The role of E2F-1 and downstream target genes in mediating ischemia/reperfusion injury in vivo.

    abstract：:E2Fs are a family of transcription factors that regulate proliferation, differentiation and apoptosis in many cell types. E2F-1 is the prototypical E2F and the family member that has most often been implicated in also mediating apoptosis. To better understand the role of E2F-1 in mediating cardiomyocyte injury we init...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2011.09.012

    authors： Angelis E,Zhao P,Zhang R,Goldhaber JI,Maclellan WR

    更新日期：2011-12-01 00:00:00

  • Altered fibroblast function following myocardial infarction.

    abstract：:Adequate wound healing and scar formation is an essential response to myocardial infarction (MI), and fibroblasts are primary cellular components regulating the process. How fibroblast functions are altered post-MI and to what extent these abnormalities persist in vitro is not well understood. Accordingly, we isolated...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.07.008

    authors： Squires CE,Escobar GP,Payne JF,Leonardi RA,Goshorn DK,Sheats NJ,Mains IM,Mingoia JT,Flack EC,Lindsey ML

    更新日期：2005-10-01 00:00:00

  • Metabolic inhibition activates a non-selective current through connexin hemichannels in isolated ventricular myocytes.

    abstract：:Intracellular Na(+)accumulation and K(+)loss play important roles in the pathogenesis of arrhythmias and injury in the ischemic heart. We investigated the role of metabolically sensitive connexin hemichannels as a potential route for Na(+)influx and K(+)efflux during ischemia, using dye uptake and electrophysiological...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2000.1220

    authors： Kondo RP,Wang SY,John SA,Weiss JN,Goldhaber JI

    更新日期：2000-10-01 00:00:00

  • Palmitoyl lysophosphatidylcholine mediated mobilization of LPL to the coronary luminal surface requires PKC activation.

    abstract：:Following diazoxide (DZ) induced hypoinsulinemia, cardiac luminal lipoprotein lipase (LPL) increases [Cardiovasc. Res. 3 (2003) 788]. To identify circulating mediators that maintain high LPL in vivo, DZ hearts were perfused for 1 h in the presence or absence of glucose, triglyceride (TG), palmitic acid or palmitoyl ly...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.07.003

    authors： Pulinilkunnil T,An D,Yip P,Chan N,Qi D,Ghosh S,Abrahani A,Rodrigues B

    更新日期：2004-11-01 00:00:00

  • Control of intracellular ionized calcium concentration by sarcolemmal and intracellular mechanisms.

    abstract：:The regulation of the resting intracellular ionized calcium concentration [( Ca2+]i) has been studied in ferret papillary muscle using the photoprotein aequorin to measure [Ca2+]i. Elevating [Ca2+]o produced an initial rapid increase of [Ca2+]i and tension which then decayed to a steady level. This secondary fall of [...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(84)80702-6

    authors： Eisner DA,Orchard CH,Allen DG

    更新日期：1984-02-01 00:00:00

  • S100A1 in cardiovascular health and disease: closing the gap between basic science and clinical therapy.

    abstract：:Calcium (Ca(2+)) signaling plays a major role in a wide range of physiological functions including control and regulation of cardiac and skeletal muscle performance and vascular tone. As all Ca(2+) signals require proteins to relay intracellular Ca(2+) oscillations downstream to different signaling networks, a specifi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.06.003

    authors： Kraus C,Rohde D,Weidenhammer C,Qiu G,Pleger ST,Voelkers M,Boerries M,Remppis A,Katus HA,Most P

    更新日期：2009-10-01 00:00:00

  • What is the mitochondrial permeability transition pore?

    abstract：:Under conditions of mitochondrial calcium overload, especially when accompanied by oxidative stress, elevated phosphate concentrations and adenine nucleotide depletion, a non-specific pore, the mitochondrial permeability transition pore (MPTP), opens in the inner mitochondrial membrane. MPTP opening enables free passa...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.02.021

    authors： Halestrap AP

    更新日期：2009-06-01 00:00:00

  • Histochemical and biochemical analysis of myosin heavy chain expression during cardiogenesis in the rat.

    abstract：:Little is known about the tissue-specific expression of contractile proteins during cardiogenesis in the mammalian heart. Since the myosin heavy chain (HC) isoform expressed in the adult correlates with myocardial functional capacity, we undertook an analysis of myosin HC expression in atrial and ventricular myocardia...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(90)91469-n

    authors： Sweeney LJ,Kelley SW

    更新日期：1990-03-01 00:00:00

  • Selective activation of the "b" splice variant of phospholipase Cbeta1 in chronically dilated human and mouse atria.

    abstract：:Atrial fibrillation (AF) is commonly associated with chronic dilatation of the left atrium, both in human disease and animal models. The immediate signaling enzyme phospholipase C (PLC) is activated by mechanical stretch to generate the Ca2+-releasing messenger inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) and sn-1,2-di...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.08.020

    authors： Woodcock EA,Grubb DR,Filtz TM,Marasco S,Luo J,McLeod-Dryden TJ,Kaye DM,Sadoshima J,Du XJ,Wong C,McMullen JR,Dart AM

    更新日期：2009-11-01 00:00:00

  • Atorvastatin-induced increase in progenitor cell levels is rather caused by enhanced receptor activator of NF-kappaB ligand (RANKL) cell proliferation than by bone marrow mobilization.

    abstract：BACKGROUND:Statins have been shown to increase the level of circulating progenitor cells in peripheral blood supposedly due to a mobilization of progenitor cells from the bone marrow niche. Osteoclast/osteoblast interaction has been associated with progenitor cell mobilization. Here, we investigated the role of statins...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.12.018

    authors： Steinmetz M,Pelster B,Lucanus E,Arnal JF,Nickenig G,Werner N

    更新日期：2013-04-01 00:00:00

  • Human connexin31.9, unlike its orthologous protein connexin30.2 in the mouse, is not detectable in the human cardiac conduction system.

    abstract：:In the human heart connexin(Cx)40, Cx43 and Cx45-containing gap junctional channels electrically couple cardiomyocytes, forming a functional syncytium. In the mouse heart, additionally, Cx30.2-containing gap junctions have been detected in the atrioventricular node where they are implicated, together with Cx45, in imp...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.12.007

    authors： Kreuzberg MM,Liebermann M,Segschneider S,Dobrowolski R,Dobrzynski H,Kaba R,Rowlinson G,Dupont E,Severs NJ,Willecke K

    更新日期：2009-04-01 00:00:00

  • Alpha-myosin heavy chain cDNA structure and gene expression in adult, fetal, and premature baboon myocardium.

    abstract：:To examine cardiac myosin gene structure and expression in a non-human primate model for human heart development and disease, we have constructed a cDNA library from baboon atrium and used baboon beta-myosin heavy chain (beta-MHC)* cDNA probes to isolate atrial MHC clones. The nucleotide sequence of one such clone, la...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(89)90805-5

    authors： Hixson JE,Henkel RD,Britten ML,Vernier DT,deLemos RA,VandeBerg JL,Walsh RA

    更新日期：1989-10-01 00:00:00

  • Quantitation and distribution of beta-tubulin in human cardiac myocytes.

    abstract：:Increasing evidence suggests that derangements of cytoskeletal proteins contribute to alterations in intracellular signaling, myocyte function, and the coupling of myocytes to the extracellular matrix during cardiac hypertrophy and failure. Data from animal studies have shown an increased density of beta-tubulin prote...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2002.2105

    authors： Aquila-Pastir LA,DiPaola NR,Matteo RG,Smedira NG,McCarthy PM,Moravec CS

    更新日期：2002-11-01 00:00:00

  • Differential reduction of HCN channel activity by various types of lipopolysaccharide.

    abstract：:Recently it was shown that lipopolysaccharide (LPS) impairs the pacemaker current in human atrial myocytes. It was speculated that reduced heart rate variability (HRV), typical of patients with severe sepsis, may partially be explained by this impairment. We evaluated the effect of various types of LPS on the activity...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2011.05.004

    authors： Klöckner U,Rueckschloss U,Grossmann C,Ebelt H,Müller-Werdan U,Loppnow H,Werdan K,Gekle M

    更新日期：2011-08-01 00:00:00

  • Sall1 transiently marks undifferentiated heart precursors and regulates their fate.

    abstract：:Cardiac progenitor cells (CPCs) are a crucial source of cells in cardiac development and regeneration. However, reported CPCs are heterogeneous, and no gene has been identified to transiently mark undifferentiated CPCs throughout heart development. Here we show that Spalt-like gene 1 (Sall1), a zing-finger transcripti...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2016.02.008

    authors： Morita Y,Andersen P,Hotta A,Tsukahara Y,Sasagawa N,Hayashida N,Koga C,Nishikawa M,Saga Y,Evans SM,Koshiba-Takeuchi K,Nishinakamura R,Yoshida Y,Kwon C,Takeuchi JK

    更新日期：2016-03-01 00:00:00

  • Shear stress-induced activation of Tie2-dependent signaling pathway enhances reendothelialization capacity of early endothelial progenitor cells.

    abstract：:Although endothelial progenitor cells (EPCs) play a pivotal role in the endothelial repair following arterial injury and shear stress has a beneficial effect on EPCs, however, the molecular mechanism underlying the influence of EPCs on the endothelial integrity and the regulation of shear stress on the EPC signaling r...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.01.019

    authors： Yang Z,Xia WH,Zhang YY,Xu SY,Liu X,Zhang XY,Yu BB,Qiu YX,Tao J

    更新日期：2012-05-01 00:00:00

  • Beta-adrenergic receptor blockade modulates Bcl-X(S) expression and reduces apoptosis in failing myocardium.

    abstract：:The mechanisms by which beta-adrenergic receptor (beta-AR) blockade modulates apoptosis in heart failure (HF) are unclear. We examined the impact of beta-AR blockade with metoprolol on myocardial remodeling, apoptosis, pro-apoptotic (Fas, Fas ligand, Bax, and Bcl-X(S)) and anti-apoptotic (Bcl-X(L)and Bcl-2) gene expre...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(03)00052-x

    authors： Prabhu SD,Wang G,Luo J,Gu Y,Ping P,Chandrasekar B

    更新日期：2003-05-01 00:00:00

  • Role of a KCNH2 polymorphism (R1047 L) in dofetilide-induced Torsades de Pointes.

    abstract：:Various drugs are reported to prolong the QT-interval on the surface ECG, thereby increasing the risk of developing a potentially fatal arrhythmia known as Torsades de Pointes (TdP). TdP case reports for these drugs have often been associated with risk factors such as overdosing, concomitant drugs and/or existing path...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.09.001

    authors： Sun Z,Milos PM,Thompson JF,Lloyd DB,Mank-Seymour A,Richmond J,Cordes JS,Zhou J

    更新日期：2004-11-01 00:00:00

  • Toll-like receptor 4 activation promotes cardiac arrhythmias by decreasing the transient outward potassium current (Ito) through an IRF3-dependent and MyD88-independent pathway.

    abstract：:Cardiac arrhythmias are one of the main causes of death worldwide. Several studies have shown that inflammation plays a key role in different cardiac diseases and Toll-like receptors (TLRs) seem to be involved in cardiac complications. In the present study, we investigated whether the activation of TLR4 induces cardia...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.08.012

    authors： Monnerat-Cahli G,Alonso H,Gallego M,Alarcón ML,Bassani RA,Casis O,Medei E

    更新日期：2014-11-01 00:00:00

  • Targeting cardiac fibroblasts to treat fibrosis of the heart: focus on HDACs.

    abstract：:Cardiac fibrosis is implicated in numerous physiologic and pathologic conditions, including scar formation, heart failure and cardiac arrhythmias. However the specific cells and signaling pathways mediating this process are poorly understood. Lysine acetylation of nucleosomal histone tails is an important mechanism fo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2014.02.015

    authors： Schuetze KB,McKinsey TA,Long CS

    更新日期：2014-05-01 00:00:00

  • A study of the effects of substrates on intracellular pH in toad ventricular strips.

    abstract：:The ability of different substrates to affect myocardial function is well established but the mechanism for this effect has yet to be determined. To explore this area further, the studies described below were designed to determine the effect of different metabolic substrates, glucose or pyruvate, on myocardial respons...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80943-9

    authors： Snow TR

    更新日期：1986-07-01 00:00:00

  • Cytotoxic T lymphocytes are activated following myocardial infarction and can recognize and kill healthy myocytes in vitro.

    abstract：:The damage of myocardial infarction (MI) is often progressive. A possible mechanism for subsequent myocardial damage and heart failure after MI is immune response against cardiac self-antigens. The purpose of our study was to test the hypothesis that cytotoxic T lymphocytes are activated following acute MI and may hav...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2000.1261

    authors： Varda-Bloom N,Leor J,Ohad DG,Hasin Y,Amar M,Fixler R,Battler A,Eldar M,Hasin D

    更新日期：2000-12-01 00:00:00

  • Mutation that dramatically alters rat titin isoform expression and cardiomyocyte passive tension.

    abstract：:Titin is a very large alternatively spliced protein that performs multiple functions in heart and skeletal muscles. A rat strain is described with an autosomal dominant mutation that alters the isoform expression of titin. While wild type animals go through a developmental program where the 3.0 MDa N2B becomes the maj...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.02.272

    authors： Greaser ML,Warren CM,Esbona K,Guo W,Duan Y,Parrish AM,Krzesinski PR,Norman HS,Dunning S,Fitzsimons DP,Moss RL

    更新日期：2008-06-01 00:00:00

  • BNip3 and signal-specific programmed death in the heart.

    abstract：:The BH3-only proteins, including BNip3, are members of the Bcl-2 family of cell death-regulating factors. Whereas proteins such as Bax and Bak play a central role in most forms of apoptosis, the BH3-only proteins appear to modulate apoptosis through cell type- and signal-specific pathways. This review will focus on ou...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2004.11.007

    authors： Webster KA,Graham RM,Bishopric NH

    更新日期：2005-01-01 00:00:00