Essential role of hIST1 in cytokinesis.

abstract：:The kinetochore is a crucial structure for faithful chromosome segregation during mitosis and is formed in the centromeric region of each chromosome. The 16-subunit protein complex known as the constitutive centromere-associated network (CCAN) forms the foundation for kinetochore assembly on the centromeric chromatin....

journal_title：Molecular biology of the cell

authors： Nagpal H,Hori T,Furukawa A,Sugase K,Osakabe A,Kurumizaka H,Fukagawa T

更新日期：2015-11-01 00:00:00

abstract：:Hsc70 with its cochaperone, either auxilin or GAK, not only uncoats clathrin-coated vesicles but also acts as a chaperone during clathrin-mediated endocytosis. However, because synaptojanin is also involved in uncoating, it is not clear whether GAK is an essential gene. To answer this question, GAK conditional knockou...

journal_title：Molecular biology of the cell

authors： Lee DW,Zhao X,Yim YI,Eisenberg E,Greene LE

更新日期：2008-07-01 00:00:00

abstract：:Mutations in the cytolinker protein plectin lead to grossly distorted morphology of neuromuscular junctions (NMJs) in patients suffering from epidermolysis bullosa simplex (EBS)-muscular dystrophy (MS) with myasthenic syndrome (MyS). Here we investigated whether plectin contributes to the structural integrity of NMJs ...

journal_title：Molecular biology of the cell

authors： Mihailovska E,Raith M,Valencia RG,Fischer I,Al Banchaabouchi M,Herbst R,Wiche G

更新日期：2014-12-15 00:00:00

abstract：:During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understo...

journal_title：Molecular biology of the cell

authors： Xie S,Mason FM,Martin AC

更新日期：2016-11-07 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00

abstract：:A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is required for gonad mo...

journal_title：Molecular biology of the cell

authors： Yoshina S,Sakaki K,Yonezumi-Hayashi A,Gengyo-Ando K,Inoue H,Iino Y,Mitani S

更新日期：2012-05-01 00:00:00

abstract：:Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic ...

journal_title：Molecular biology of the cell

authors： Faigle W,Colucci-Guyon E,Louvard D,Amigorena S,Galli T

更新日期：2000-10-01 00:00:00

abstract：:Human HT2-19 cells with a conditional cdk1 mutation stop dividing upon cdk1 inactivation and undergo multiple rounds of endoreplication. We show herein that major cell cycle events remain synchronized in these endoreplicating cells. DNA replication alternates with gap phases and cell cycle-specific cyclin E expression...

journal_title：Molecular biology of the cell

authors： Laronne A,Rotkopf S,Hellman A,Gruenbaum Y,Porter AC,Brandeis M

更新日期：2003-09-01 00:00:00

abstract：:The fission yeast Mcs6-Mcs2-Pmh1 complex, homologous to metazoan Cdk7-cyclin H-Mat1, has dual functions in cell division and transcription: as a partially redundant cyclin-dependent kinase (CDK)-activating kinase (CAK) that phosphorylates the major cell cycle CDK, Cdc2, on Thr-167; and as the RNA polymerase (Pol) II c...

journal_title：Molecular biology of the cell

authors： Lee KM,Miklos I,Du H,Watt S,Szilagyi Z,Saiz JE,Madabhushi R,Penkett CJ,Sipiczki M,Bähler J,Fisher RP

更新日期：2005-06-01 00:00:00

abstract：:The actin cytoskeleton is constantly assembling and disassembling. Cells harness the energy of these turnover dynamics to drive cell motility and organize cytoplasm. Although much is known about how cells control actin polymerization, we do not understand how actin filaments depolymerize inside cells. I briefly descri...

journal_title：Molecular biology of the cell

authors： Brieher W

更新日期：2013-08-01 00:00:00

abstract：:Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and sorted to peroxisomes. These mutants also have a glutamate or aspartate requirement on minimal media. Cloning of the cor...

journal_title：Molecular biology of the cell

authors： Ozimek P,van Dijk R,Latchev K,Gancedo C,Wang DY,van der Klei IJ,Veenhuis M

更新日期：2003-02-01 00:00:00

abstract：:Microtubules (MTs) are often organized by a nucleus-associated MT organizing center (MTOC). In addition, in neurons and epithelial cells, motor-based transport of assembled MTs determines the polarity of the MT array. Here, we show that MT motility participates in MT organization in the fungus Ustilago maydis. In budd...

journal_title：Molecular biology of the cell

authors： Fink G,Steinberg G

更新日期：2006-07-01 00:00:00

abstract：:Calcium (Ca(2+)) and calmodulin (CaM) are required for progression of mammalian cells from quiescence into S phase. In multiple cell types, cyclosporin A causes a G(1) cell cycle arrest, implicating the serine/threonine phosphatase calcineurin as one Ca(2+)/CaM-dependent enzyme required for G(1) transit. Here, we show...

journal_title：Molecular biology of the cell

authors： Kahl CR,Means AR

更新日期：2004-04-01 00:00:00

abstract：:Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

journal_title：Molecular biology of the cell

authors： Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

更新日期：2020-11-01 00:00:00

abstract：:Cystic fibrosis results from mutations in the cystic fibrosis conductance regulator protein (CFTR), a cAMP/protein kinase A (PKA) and ATP-regulated Cl(-) channel. CFTR is increasingly recognized as a component of multiprotein complexes and although several inhibitory proteins to CFTR have been identified, protein comp...

journal_title：Molecular biology of the cell

authors： Borthwick LA,McGaw J,Conner G,Taylor CJ,Gerke V,Mehta A,Robson L,Muimo R

更新日期：2007-09-01 00:00:00

abstract：:The ubiquitin-like hPLIC proteins can associate with proteasomes, and hPLIC overexpression can specifically interfere with ubiquitin-mediated proteolysis (Kleijnen et al., 2000). Because the hPLIC proteins can also interact with certain E3 ubiquitin protein ligases, they may provide a link between the ubiquitination a...

journal_title：Molecular biology of the cell

authors： Kleijnen MF,Alarcon RM,Howley PM

更新日期：2003-09-01 00:00:00

abstract：:Here we demonstrate that multiple tetraspanin (transmembrane 4 superfamily) proteins are palmitoylated, in either the Golgi or a post-Golgi compartment. Using CD151 as a model tetraspanin, we identified and mutated intracellular N-terminal and C-terminal cysteine palmitoylation sites. Simultaneous mutations of C11, C1...

journal_title：Molecular biology of the cell

authors： Yang X,Claas C,Kraeft SK,Chen LB,Wang Z,Kreidberg JA,Hemler ME

更新日期：2002-03-01 00:00:00

abstract：:Surface charges at the inner leaflet of the plasma membrane may contribute to regulate the surface recruitment of key signaling factors. Phosphatidylserine (PS) is an abundant charged lipid that may regulate charge distribution in different cell types. Here we characterize the subcellular distribution and function of ...

journal_title：Molecular biology of the cell

authors： Haupt A,Minc N

更新日期：2017-01-01 00:00:00

abstract：:The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here, we microinjected epitope-tagged (Myc and Flag) cDNAs for ...

journal_title：Molecular biology of the cell

authors： Qi H,Williams Z,Wassarman PM

更新日期：2002-02-01 00:00:00

abstract：:Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 G...

journal_title：Molecular biology of the cell

authors： Peden AA,Schonteich E,Chun J,Junutula JR,Scheller RH,Prekeris R

更新日期：2004-08-01 00:00:00

abstract：:The beta 2 integrin lymphocyte function-associated antigen 1 (LFA-1) mediates activation-dependent adhesion of lymphocytes. To investigate whether lymphocyte-specific elements are essential for LFA-1 function, we expressed LFA-1 in the erythroleukemic cell line K562, which expresses only the integrin very late antigen...

journal_title：Molecular biology of the cell

authors： Lub M,van Vliet SJ,Oomen SP,Pieters RA,Robinson M,Figdor CG,van Kooyk Y

更新日期：1997-04-01 00:00:00

abstract：:The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have ...

journal_title：Molecular biology of the cell

authors： Cai S,Song Y,Chen C,Shi J,Gan L

更新日期：2018-07-01 00:00:00

abstract：:Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Cae...

journal_title：Molecular biology of the cell

authors： Bar DZ,Davidovich M,Lamm AT,Zer H,Wilson KL,Gruenbaum Y

更新日期：2014-04-01 00:00:00

abstract：:A central question in the area of signal transduction is why pathways utilize common components. In the budding yeast Saccharomyces cerevisiae, the HOG and filamentous growth (FG) MAPK pathways require overlapping components but are thought to be induced by different stimuli and specify distinct outputs. To better und...

journal_title：Molecular biology of the cell

authors： Pitoniak A,Birkaya B,Dionne HM,Vadaie N,Cullen PJ

更新日期：2009-07-01 00:00:00

abstract：:The yeast [PSI+] prion is an epigenetic modifier of translation termination fidelity that causes nonsense suppression. The prion [PSI+] forms when the translation termination factor Sup35p adopts a self-propagating conformation. The presence of the [PSI+] prion modulates survivability in a variety of growth conditions...

journal_title：Molecular biology of the cell

authors： Strawn LA,Lin CA,Tank EM,Osman MM,Simpson SA,True HL

更新日期：2009-04-01 00:00:00

abstract：:Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane traffic...

journal_title：Molecular biology of the cell

authors： Tanaka Y,Ono N,Shima T,Tanaka G,Katoh Y,Nakayama K,Takatsu H,Shin HW

更新日期：2016-12-01 00:00:00

abstract：:Microtubules (MTs) have been proposed to act mechanically as compressive struts that resist both actomyosin contractile forces and their own polymerization forces to mechanically stabilize cell shape. To identify the origin of MT bending, we directly observed MT bending and F-actin transport dynamics in the periphery ...

journal_title：Molecular biology of the cell

authors： Bicek AD,Tüzel E,Demtchouk A,Uppalapati M,Hancock WO,Kroll DM,Odde DJ

更新日期：2009-06-01 00:00:00

abstract：:Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

journal_title：Molecular biology of the cell

authors： Soldati T,Riederer MA,Pfeffer SR

更新日期：1993-04-01 00:00:00

abstract：:Oncostatin M and cAMP signaling stimulate apical surface-directed membrane trafficking and apical lumen development in hepatocytes, both in a protein kinase A (PKA)-dependent manner. Here, we show that oncostatin M, but not cAMP, promotes the A-kinase anchoring protein (AKAP)-dependent anchoring of the PKA regulatory ...

journal_title：Molecular biology of the cell

authors： Wojtal KA,Hoekstra D,van Ijzendoorn SC

更新日期：2007-07-01 00:00:00

abstract：:We have identified a novel Ras-interacting protein from Dictyostelium, RIP3, whose function is required for both chemotaxis and the synthesis and relay of the cyclic AMP (cAMP) chemoattractant signal. rip3 null cells are unable to aggregate and lack receptor activation of adenylyl cyclase but are able, in response to ...

journal_title：Molecular biology of the cell

authors： Lee S,Parent CA,Insall R,Firtel RA

更新日期：1999-09-01 00:00:00