Abstract:
:A growing number of studies indicate a link between oxidative stress and cancer. We previously developed a rat model of renal cell carcinoma (RCC) induced by ferric nitrilotriacetate (Fe-NTA). Here, we performed a genome-wide analysis to study characteristics of genomic alteration and identify putative genes involved in the development of Fe-NTA-induced RCCs. Array-based comparative genomic hybridization analyses revealed a chromosomal loss spanning chromosome 8 in most of the RCCs studied, with a common deletion at 8q31-32, which was confirmed by loss of heterozygosity (LOH) analysis. Studies of gene expression in RCCs or following Fe-NTA treatment revealed globally decreased transcription levels of 34 genes derived from chromosome 8 that are expressed in the kidney. Among them, the aminoacylase 1 (Acy1) gene, which maps to 8q32 and is highly expressed in the kidney, displayed a significantly decreased level of expression in RCCs. Significant amounts of the Acy1 protein were detected in the cytoplasm as well as in the nuclei of renal proximal tubular cells of untreated rats. Transfection of Acy1 into RCC cell lines inhibited proliferation and colony formation on soft agar. An increased number of apoptotic cells were observed following Acy1 transfection. The rat 8q31-32 chromosomal region corresponds to human 3p21.31-24.1, a hot spot where LOH is frequently found in various human cancers. Thus, Fe-NTA-induced renal tumor model is ideal for studying the link between deletions within this region and tumor formation. Our data demonstrate that Acy1 functions as a tumor suppressor in this rat RCC model.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Zhong Y,Onuki J,Yamasaki T,Ogawa O,Akatsuka S,Toyokuni Sdoi
10.1093/carcin/bgn255subject
Has Abstractpub_date
2009-01-01 00:00:00pages
158-64issue
1eissn
0143-3334issn
1460-2180pii
bgn255journal_volume
30pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mutagenic and carcinogenic heterocyclic amine formed during ordinary cooking. PhIP is metabolically activated to the ultimate mutagenic metabolite by CYP P450-mediated N-hydroxylation followed by phase II esterification. Incubation of N-hydroxy-PhIP (N-OH-PhI...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::In Thailand, smoking of commercial cigarettes and of handmade cigarettes has drastically increased in recent decades. Cancer of the lung and of the upper aero-digestive tract have also increased in Thailand as they have in many other countries. It is our working hypothesis that the increase of primary cancer of the li...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.1.133
更新日期:1999-01-01 00:00:00
abstract::Genetic variants in multiple cellular pathways have been associated with an altered risk of oesophageal cancer. In this study, eight genes previously associated with an altered risk of oesophageal squamous cell carcinoma (OSCC) in European or Asian populations were investigated in two South African populations. We gen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr211
更新日期:2011-12-01 00:00:00
abstract::Synergistic effects of dysregulation of the WNT/CTNNB1 and phosphatidylinositol 3-kinase (PI3K)/AKT pathways are thought to be important for the development and progression of many forms of cancer, including the granulosa cell tumor of the ovary. Sustained WNT/CTNNB1 signaling in Sertoli cells causes testicular degene...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp051
更新日期:2009-05-01 00:00:00
abstract::Mutagenicity assays were carried out in the Salmonella/microsome test, using five S. typhimurium his(-) strains (TA1535, TA1537, TA1538, TA98 and TA100), both in the presence and absence of post-mitochondrial preparations from Aroclor-induced rat livers and suitable co-factors. Seven oil dispersants showed a wide rang...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.1.51
更新日期:1980-01-01 00:00:00
abstract::The LEC rat spontaneously develops liver cancer after suffering chronic liver injury caused by abnormal copper accumulation in the liver, but the role of copper accumulation in the induction of liver cancer remains obscure. We histochemically and biochemically examined the content of copper and metallothionein (MT), a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.9.1833
更新日期:1994-09-01 00:00:00
abstract::The spectrum of mutations induced by N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF) was examined by the pZipHprtNeo shuttle vector in mammalian cells. The vector carries a cDNA of the human hypoxanthine phosphoribosyl transferase (hprt) gene, which is stably integrated into chromosomal DNA of a mouse cell line, VH12. ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.11.2245
更新日期:1993-11-01 00:00:00
abstract::In order to detect any protective agent against tumor formation, we examined the anti-tumor-promoting effect of a Japanese traditional soybean fermented food, Natto. Dye transfer was employed as an assay method. When fluorescent dye was microinjected into cultured BALB/3T3 cells, the dye was transformed into the neigh...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.3.471
更新日期:1995-03-01 00:00:00
abstract::Colonic epithelial cell migration is required for normal differentiated cell function. This migratory phenotype is dependent upon wild-type adenomatous polyposis coli (Apc) expression. Non-tumorigenic murine colon epithelial cell lines with distinct Apc genotypes, i.e. young adult mouse colon (YAMC; Apc(+/+)) and immo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.6.1065
更新日期:2002-06-01 00:00:00
abstract::Reactive oxygen species (ROS) have been implicated in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) resistance of many cancers. We evaluated the role of peroxiredoxin (Prx) I in TRAIL resistance governed by coupling of nicotinamide adenosine dinucleotide phosphate oxidase (Nox)-derived ROS signaling ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp104
更新日期:2009-07-01 00:00:00
abstract::Sprague-Dawley rats were treated with N-nitrosomorpholine (NNM) alone (7 weeks, 120 mg/l in drinking water), with NNM followed by phenobarbital (PB) (750 mg/l for 6 weeks) or PB alone. The livers from these animals were investigated for glycogen content and activities of glucose-6-phosphatase, glucose-6-phosphate dehy...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.7.931
更新日期:1983-01-01 00:00:00
abstract::Peroxisome proliferator activated receptor(alpha) (PPARalpha) mediates the liver's responses to peroxisome proliferator compounds. These responses include induction of specific hepatic enzymes, peroxisome proliferation and hepatocyte proliferation. PPARalpha null mice, which lack receptor in all cells of the body, do ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg180
更新日期:2004-01-01 00:00:00
abstract::A three-dimensional molecular template has been generated for substrates of human debrisoquine 4-hydroxylase cytochrome P450 (CYP2D6). This template defines the stereochemical requirements for CYP2D6 substrates in terms of the volume occupied and positions of key atoms. The modelling was based on the X-ray crystallogr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.12.2211
更新日期:1991-12-01 00:00:00
abstract::Certain dietary constituents can protect against chemically induced carcinogenesis in rodents. A principal mechanism by which these chemopreventive compounds exert their protective effects is likely to be via induction of carcinogen detoxification. This can be mediated by conjugation with glutathione, which is synthes...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.10.1827
更新日期:2000-10-01 00:00:00
abstract::Semi-permeable magnetic microcapsules previously shown able to trap gastrointestinal carcinogens and containing polyethyleneimine (PEI) were covalently labelled with [14CH3], and administered for the first time to humans (six healthy volunteers, 1.3 microCi/dose) in gelatin capsules together with radio-opaque gut tran...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.4.683
更新日期:1992-04-01 00:00:00
abstract::An electron microscope study was carried out on changes in the pancreatic acinar tissues of male Syrian hamsters during the course of induction of pancreatic neoplasia by lifetime weekly injections of N-nitroso-bis(2-hydroxypropyl)amine (BHP). The dominant process observed was a loss of differentiation of the acinar c...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.5.485
更新日期:1982-01-01 00:00:00
abstract::Carcinogenic tobacco-specific nitrosamines are present in tobacco products and are believed to play a significant role in human cancers associated with tobacco use. Additional amounts of tobacco-specific nitrosamines could be formed endogenously. We tested this hypothesis by treating rats with nicotine and sodium nitr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.3.587
更新日期:1997-03-01 00:00:00
abstract::The expression of cyclooxygenase-2 (COX-2) has been reported as an indicator of poor prognosis in a wide variety of human tumors, including colon, breast and uveal melanoma (UM). COX-2 inhibitors have shown promise in controlling the malignancy of several types of tumors. Previous studies have demonstrated the efficac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm091
更新日期:2007-09-01 00:00:00
abstract::The repair of u.v.-induced damage in human and rodent cells was investigated at the level of DNA loops attached to the nuclear matrix. After 2 h post-u.v. incubation, DNase I digestion studies revealed a 3- to 4-fold enrichment of repair-labeled DNA at the nuclear matrix in four xeroderma pigmentosum cell strains belo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.6.995
更新日期:1986-06-01 00:00:00
abstract::Rats received a choline-deficient diet containing 0.1% (w/w) DL-ethionine (CDE) for 4, 10, 14 or 22 weeks. A separate group was treated for 4 weeks with CDE and then received a normal diet for 4 weeks. The L and M2 isoenzymes of pyruvate kinase were immunocytochemically demonstrated in liver sections. L-PK expression ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.1.99
更新日期:1998-01-01 00:00:00
abstract::In 2000, Douglas Hanahan and Robert Weinberg published a review detailing the six hallmarks of cancer. These are six phenotypes that a tumour requires in order to become a fully fledged malignancy: persistent growth signals, evasion of apoptosis, insensitivity to anti-growth signals, unlimited replicative potential, a...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgp070
更新日期:2009-08-01 00:00:00
abstract::Despite significant research, our understanding of the molecular mechanisms of Human Papilloma Virus (HPV) induced cancers remains incomplete. Majority of invasive cervical cancers are caused by high-risk HPV 16 and 18. Two potent HPV oncoproteins, E6 and E7, promote human malignancies by disrupting the activities of ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu104
更新日期:2014-08-01 00:00:00
abstract::The effect of glucose on the formation of the food mutagen PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) was studied in a model system. When a mixture of creatine (0.9 mmol), phenylalanine (0.9 mmol) and glucose (0.45 mmol) was heated in diethylene glycol and water (3 ml, 5:1) for 10 min at 180 or 225 degrees...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.12.2297
更新日期:1991-12-01 00:00:00
abstract::This study presents the physical characterization of mutants induced in mammalian cells by high linear energy transfer alpha-particle radiation that simulates exposure to radon daughters. Alpha-Particles from accelerated 4He at 150 keV/microns were used to induce 20 Chinese hamster ovary mutants that are deficient in ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.8.1981
更新日期:1995-08-01 00:00:00
abstract::Macrophages and their immortalized cell lines can be activated to form nitrite and nitrate via oxidation of arginine and this is accompanied by the formation of N-nitroso compounds. The mechanism of nitrosamine formation has been investigated through the use of compounds which are known either to inhibit or enhance ac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.3.563
更新日期:1989-03-01 00:00:00
abstract::Lack of functional telomeres can cause chromosomal aberrations. This type of genetic instability may promote tumorigenesis. We have investigated the association between mean telomere length in buccal cells (assessed with quantitative real-time PCR) and bladder cancer risk in a case-control study. Patients with bladder...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi063
更新日期:2005-07-01 00:00:00
abstract::Exposure of rats to diesel emissions results in the development of lung tumors. The objective of this study was to determine whether the polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs or other polycyclic organic matter adsorbed to diesel particles induces the formation of DNA adducts in the lung when compared to ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.7.1291
更新日期:1994-07-01 00:00:00
abstract::A series of cells representing normal, non-tumorigenic cell lines, as well as differentiating neoplastic and undifferentiated neoplastic rat tracheal epithelial cell populations were evaluated for their ability to establish homologous and/or heterologous cell-cell gap junction communication in culture. Gap junction co...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.11.2043
更新日期:1997-11-01 00:00:00
abstract::This commentary celebrates the 40th year of Carcinogenesis, spanning 1980-2020 with a focus on lung cancer. For lung cancer, these 40 years come toward the end of a century of scientific inquiry that began with descriptions of this highly fatal malignancy and that closes with emphasis on molecular processes and genomi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa094
更新日期:2020-10-15 00:00:00
abstract::SENCAR mice are unusually sensitive to induction of papillomas and squamous cell carcinomas by initiation with 7,12-dimethylbenzanthracene (DMBA) and promotion by 12-O-tetradecanoylphorbol-13-acetate (TPA). Tumors induced by this protocol were tested for the presence of papillomavirus by immunohistochemistry, Southern...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.2.341
更新日期:1990-02-01 00:00:00