Abstract:
BACKGROUND:Blood alcohol determination plays an important role in laboratory medicine and forensic medicine. Nowadays, many methods are being used for alcohol measurement, but these methods are time-consuming and complex to perform laborious sample pre-treatment. The disposable amperometric biosensor, due to its portability, low cost and potential for fabrication, should be readily applicable for blood alcohol determination. METHODS:The biosensor was fabricated by immobilizing alcohol dehydrogenase and nicotinamide adenine dinucleotide coated by Nafion combined with gold nanoparticles onto the surface of screen-printed electrode modified with Meldola's blue. Evaluations of biosensor performance were performed according to the relevant National Committee for Clinical Laboratory Standards standard. RESULTS:The biosensor response for serum alcohol presents good linearity, precision, stability, accuracy, and specificity. CONCLUSIONS:The biosensor exhibits the capability of detecting blood alcohol concentration in the clinical laboratory and in forensic medicine, unnecessarily performing laborious sample pre-treatment.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Luo P,Xie G,Liu Y,Xu H,Deng S,Song Fdoi
10.1515/CCLM.2008.308subject
Has Abstractpub_date
2008-01-01 00:00:00pages
1641-7issue
11eissn
1434-6621issn
1437-4331pii
10.1515/CCLM.2008.308journal_volume
46pub_type
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