Growth characteristics and enzyme activity in Batrachochytrium dendrobatidis isolates.

Abstract:

:Batrachochytrium dendrobatidis is a member of the phylum Chytridiomycota and the causative organism chytridiomycosis, a disease of amphibians associated with global population declines and mass mortality events. The organism targets keratin-forming epithelium in adult and larval amphibians, which suggests that keratinolytic activity may be required to infect amphibian hosts. To investigate this hypothesis, we tested 10 isolates of B. dendrobatidis for their ability to grow on a range of keratin-supplemented agars and measured keratolytic enzyme activity using a commercially available kit (bioMerieux API ZYM). The most dense and fastest growth of isolates were recorded on tryptone agar, followed by growth on frog skin agar and the slowest growth recorded on feather meal and boiled snake skin agar. Growth patterns were distinctive for each nutrient source. All 10 isolates were strongly positive for a range of proteolytic enzymes which may be keratinolytic, including trypsin and chymotrypsin. These findings support the predilection of B. dendrobatidis for amphibian skin.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Symonds EP,Trott DJ,Bird PS,Mills P

doi

10.1007/s11046-008-9135-y

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

143-7

issue

3

eissn

0301-486X

issn

1573-0832

journal_volume

166

pub_type

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