Prevention of CMV-induced myelosuppression by anti-CMV antibodies: an in vitro model.

abstract：OBJECTIVES:Using metaphase cytogenetics (MC), chromosomal defects can be detected in 40% to 60% of patients with myelodysplastic syndromes (MDS); cytogenetic results have a major impact on prognosis. We hypothesize that more precise methods of chromosomal analysis will detect new/additional cryptic lesions in a higher ...

journal_title：Experimental hematology

authors： Gondek LP,Haddad AS,O'Keefe CL,Tiu R,Wlodarski MW,Sekeres MA,Theil KS,Maciejewski JP

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVE:Common marmosets are considered experimental animals of primates useful for medical research. We developed several monoclonal antibodies (mAbs) directed to CD molecules to gain initial insight into the immune and hematopoietic systems of this organism, and analyzed the basic cellularity and characters of marm...

journal_title：Experimental hematology

authors： Kametani Y,Suzuki D,Kohu K,Satake M,Suemizu H,Sasaki E,Ito T,Tamaoki N,Mizushima T,Ozawa M,Tani K,Kito M,Arai H,Koyanagi A,Yagita H,Habu S

更新日期：2009-11-01 00:00:00

abstract：:Blood samples were examined from 25 children with malignancies during hematopoietic recovery following chemotherapy-induced aplasia and from 9 children undergoing tonsillectomy. The proportion of CD34-positive peripheral blood mononuclear cells (PBMNC) evaluated by flow cytometry was compared with the number of coloni...

journal_title：Experimental hematology

authors： Fritsch G,Emminger W,Buchinger P,Printz D,Gadner H

更新日期：1991-12-01 00:00:00

abstract：OBJECTIVE:To investigate whether Flk1(+)CD31(-)CD34(-) cells isolated from fetal bone marrow (BM) have characteristics of hemangioblasts, i.e., progenitors of endothelial and hematopoietic cells. MATERIALS AND METHODS:Mononuclear cells from fetal BM were negatively sorted by CD45, GlyA, and CD34 micromagnetic beads, t...

journal_title：Experimental hematology

authors： Guo H,Fang B,Liao L,Zhao Z,Liu J,Chen H,Hsu SH,Cui Q,Zhao RC

更新日期：2003-07-01 00:00:00

abstract：:The origin of spleen colony-forming units (CFUs) and fibroblastoid colony-forming cells (CFUF) repopulating the marrow of femurs subcutaneously implanted in syngeneic and semi-syngeneic mice was studied by making use of a chromosome marker for CFUs and a cytotoxicity test for CFUF. The CFUs present in implants during ...

journal_title：Experimental hematology

authors： Hotta T,Murate T,Utsumi M,Hirabayashi N,Yamada H

更新日期：1983-02-01 00:00:00

abstract：:A simple two-step procedure has been developed for the production of human urinary erythropoietin suitable for tissue culture use. Lyophilized human urinary protein was fractionated by phenyl-Sepharose chromatography, the erythropoietin binding in 2 M lithium chloride and being eluted by an ascending linear gradient t...

journal_title：Experimental hematology

authors： Cutler RL,Johnson GR,Nicola NA

更新日期：1985-09-01 00:00:00

abstract：OBJECTIVE:Approximately 60% of all anaplastic large-cell lymphomas (ALCL) contain a specific t(2;5)(p23;q35) chromosomal translocation leading to overexpression of NPM-ALK. As the chimeric tyrosine kinase is involved in tumorigenesis and pathogenesis of ALCL, we were interested to inhibit NPM-ALK expression using an ex...

journal_title：Experimental hematology

authors： Hübinger G,Wehnes E,Xue L,Morris SW,Maurer U

更新日期：2003-03-01 00:00:00

abstract：OBJECTIVE:Mechanisms of T-cell stimulation by Flt3 ligand (Flt3L) and granulocyte-macrophage colony-stimulating factor (GM-CSF) remain unclear. Herein, we compared the effects of Flt3L and GM-CSF on the expansion of dendritic cells (DC) and T-cell subsets and cytokine expression. METHODS:Naïve and effector/memory T ce...

journal_title：Experimental hematology

authors： Parajuli P,Mosley RL,Pisarev V,Chavez J,Ulrich A,Varney M,Singh RK,Talmadge JE

更新日期：2001-10-01 00:00:00

abstract：:Fourteen patients with chronic granulocytic leukemia received bone marrow grafts from HLA identical siblings. Ten patients were in blast crisis prior to grafting, three were in an accelerated phase of their disease, and one was aplastic secondary to chemotherapy. Prior to transplant all patients were conditioned with ...

journal_title：Experimental hematology

authors： Doney K,Buckner CD,Sale GE,Ramberg R,Boyd C,Thomas ED

更新日期：1978-10-01 00:00:00

abstract：:Delayed platelet recovery and altered marrow megakaryocytopoiesis was observed in highly irradiated mice following transplantation with 1000 lineage-negative, stem cell antigen-positive (Lin-Sca-1+) cells. Thirty days after transplantation, the mice were thrombocytopenic. Normal platelet levels were reestablished by 9...

journal_title：Experimental hematology

authors： Bradford G,Williams N,Barber L,Bertoncello I

更新日期：1993-12-01 00:00:00

abstract：OBJECTIVE:Our laboratory reported that the angiotensin converting enzyme inhibitor captopril improves erythroid recovery from total body irradiation (TBI) in mice when administered after irradiation. However, captopril administered before TBI attenuates erythroid recovery. Here we investigate captopril and radiation re...

journal_title：Experimental hematology

authors： Barshishat-Kupper M,Mungunsukh O,Tipton AJ,McCart EA,Panganiban RA,Davis TA,Landauer MR,Day RM

更新日期：2011-03-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) can be generated from various types of cells with transduction of defined transcription factors. Patient-derived iPSCs are becoming commonly utilized for understanding the molecular pathways involved in disease and for the development of novel targeted therapies. With the use of ...

journal_title：Experimental hematology

authors： Arai S,Miyauchi M,Kurokawa M

更新日期：2015-08-01 00:00:00

abstract：:Mantle cell lymphoma (MCL) affects approximately 4500 patients/year in the US and demonstrates a male to female ratio of approximately 4:1. While the pathobiology underlying this ratio is unknown, the hematopoietic system is characterized by sex-related differences in androgen receptor (AR) expression, leading us to h...

journal_title：Experimental hematology

authors： Mostaghel EA,Martin PS,Mongovin S,Frayo S,Zhang A,Edlefsen KL,Press OW,Gopal AK

更新日期：2017-05-01 00:00:00

abstract：:The heterogeneity among progenitor cells of granulocytes and macrophages capable of forming colonies in vitro (CFU-c) is analysed. If specific culture conditions and stimuli are used, it is possible to discriminate among three subpopulations of CFU-c. CFU-c directly responding to CSF prepared from pregnant mouse uteri...

journal_title：Experimental hematology

authors： Bol S,Visser J,van den Engh G

更新日期：1979-11-01 00:00:00

abstract：:The retroviral vector N2, which is derived from the Moloney murine leukemia retrovirus, was used to transfer the bacterial NeoR gene (conferring resistance to the neomycin analogue G418) into hematopoietic progenitor cells from fetal, neonatal, and adult dogs and cats. Infection of canine and feline bone marrow cells ...

journal_title：Experimental hematology

authors： al-Lebban ZS,Henry JM,Jones JB,Eglitis MA,Anderson WF,Lothrop CD Jr

更新日期：1990-03-01 00:00:00

abstract：:Hepcidin is the key regulator of iron absorption and recycling, and its expression is suppressed by red blood cell production. When erythropoiesis is expanded, hepcidin expression decreases. To gain insight into the stage of erythroid differentiation at which the regulation might originate, we measured serum hepcidin ...

journal_title：Experimental hematology

authors： Keel SB,Doty R,Liu L,Nemeth E,Cherian S,Ganz T,Abkowitz JL

更新日期：2015-06-01 00:00:00

abstract：:Progress of age-related hematopoietic diseases such as myelodysplastic syndrome has previously been linked to enhanced levels of reactive oxygen species (ROS). Uncoupling protein 2 (UCP2) was found to reduce mitochondrial ROS production through uncoupling of the respiratory chain. The impact of UCP2 loss and elevated ...

journal_title：Experimental hematology

authors： Kretzschmar C,Roolf C,Timmer K,Sekora A,Knübel G,Murua Escobar H,Jaster R,Müller S,Fuellen G,Köhling R,Junghanss C

更新日期：2016-11-01 00:00:00

abstract：:More than 2million human erythroblasts extrude their nuclei every second in bone marrow under hypoxic conditions (<7% O2). Enucleation requires specific signal transduction pathways and the local assembly of contractile actomyosin rings. However, the energy source driving these events has not yet been identified. We e...

journal_title：Experimental hematology

authors： Goto T,Ubukawa K,Kobayashi I,Sugawara K,Asanuma K,Sasaki Y,Guo YM,Takahashi N,Sawada K,Wakui H,Nunomura W

更新日期：2019-04-01 00:00:00

abstract：:Lifelong blood production is ensured by a population of rare and largely quiescent, long-lived hematopoietic stem cells (HSCs). The advent of single-cell technologies has recently highlighted underlying molecular and functional heterogeneity within the HSC pool. Despite heterogenous HSC behaviors, quiescence remains a...

journal_title：Experimental hematology

authors： Johnson C,Belluschi S,Laurenti E

更新日期：2020-12-01 00:00:00

abstract：:The aberrant overexpression of Wilms tumor-1 (WT1) in acute myeloid leukemia (AML) plays an important role in blast cell survival by enhancing proliferation and inhibiting apoptosis. However, the mechanism underlying the overexpression of WT1 remains unclear. Here, we identified miR-193a (miR-193a-5p) and miR-600 targ...

journal_title：Experimental hematology

authors： Li H,Xing C,Zhou B,Ye H,Feng J,Wu J,Gao S

更新日期：2018-05-01 00:00:00

abstract：:An increasing amount of data provides strong evidence for the complex multifactorial control of primary hemopoietic functions. Here we present a new multicellular functional unit, the Hematon, isolated from the light-density floating fraction of normal human bone marrow (BM) aspirates. The Hematon is organized in a co...

journal_title：Experimental hematology

authors： Blazsek I,Misset JL,Benavides M,Comisso M,Ribaud P,Mathé G

更新日期：1990-05-01 00:00:00

abstract：:Current next-generation sequencing (NGS) technologies allow unprecedented insights into the mutational profiles of tumors. Recent studies in myeloproliferative neoplasms have further demonstrated that, not only the mutational profile, but also the order in which these mutations are acquired is relevant for our underst...

journal_title：Experimental hematology

authors： Nice FL,Massie CE,Klampfl T,Green AR

更新日期：2018-01-01 00:00:00

abstract：OBJECTIVE:B-cell chronic lymphocytic leukemia is a heterogeneous disease with a pronounced variation in the clinical course. With the purpose of identifying genes that could be related to disease progression, we have performed gene expression profiling on B-CLL patients with an indolent disease and patients with a prog...

journal_title：Experimental hematology

authors： Fält S,Merup M,Gahrton G,Lambert B,Wennborg A

更新日期：2005-08-01 00:00:00

abstract：OBJECTIVES:To analyze the consequences of lymphocyte donations on lymphopoiesis in donors having previously undergone hematopoietic stem cell collection for allogeneic stem cell transplantation. METHODS:Repeated analysis of leukocyte subsets in the peripheral blood of 76 hematopoietic stem cell donors undergoing lymph...

journal_title：Experimental hematology

authors： Nicolini FE,Wattel E,Michallet AS,Bourgeot JP,Tremisi JP,Hequet O,Michallet M

更新日期：2004-11-01 00:00:00

abstract：:Previously, recombinant erythropoietin (rEpo) was shown to increase the number and size of megakaryocytic colonies in vitro, and in vivo it elevates the number of megakaryocytes in mouse spleens. To test the hypothesis that rEpo would stimulate platelet production in mice, both normal mice and mice in rebound-thromboc...

journal_title：Experimental hematology

authors： McDonald TP,Cottrell MB,Clift RE,Cullen WC,Lin FK

更新日期：1987-07-01 00:00:00

abstract：OBJECTIVE:Resveratrol (3,4',5,-trihydroxystilbene, RV), an ingredient of wine, is an inhibitor of the proliferation-linked enzyme ribonucleotide reductase (RR) and shows a broad spectrum of cytotoxic effects against human cancer cells. In order to enhance these effects, we introduced additional hydroxyl moieties into t...

journal_title：Experimental hematology

authors： Horvath Z,Murias M,Saiko P,Erker T,Handler N,Madlener S,Jaeger W,Grusch M,Fritzer-Szekeres M,Krupitza G,Szekeres T

更新日期：2006-10-01 00:00:00

abstract：:Erythropoietin (Epo), a glycoprotein hormone, regulates the proliferation and differentiation of committed erythroid progenitor cells. We investigated the effect of Epo on the kinetics of an Epo-dependent cell line (DA-1ER), which was cloned from the murine interleukin 3-dependent cell line, DA-1. Flow cytometry and [...

journal_title：Experimental hematology

authors： Tsuda H,Sawada T,Kawakita M,Takatsuki K

更新日期：1989-03-01 00:00:00

abstract：OBJECTIVE:Donor lymphocyte transfusions (DLT) are effective in the treatment of leukemia after allogeneic stem cell transplantation. Graft-vs-host-disease (GVHD) is the major risk factor of DLT. In dog leukocyte antigen (DLA)-identical littermate dogs, DLT given within 3 weeks after transplantation of marrow depleted o...

journal_title：Experimental hematology

authors： Zorn J,Herber M,Schwamberger S,Panzer W,Adler H,Kolb HJ

更新日期：2009-08-01 00:00:00

abstract：:Clonal lines of mouse inducer ly1+ly2- inducer T-lymphocytes that depend for growth upon interleukin-2 have been demonstrated to produce a factor that stimulates colony formation by bone marrow granulocyte-macrophage (GM-CFUc) progenitor cells and replication of factor-dependent mast cell/basophil and multipotential h...

journal_title：Experimental hematology

authors： Greenberger JS,Humphries RK,Messner H,Reid DM,Sakakeeny MA

更新日期：1985-05-01 00:00:00

abstract：:Although three molecular forms of macrophage colony-stimulating factor (M-CSF) have been reported, Western blot analysis of immunoaffinity-purified M-CSF from human blood has shown that the major species of M-CSF in the serum has a molecular weight (MW) of 85 kD. Superose-12 gel filtration chromatography of immunoaffi...

journal_title：Experimental hematology

authors： Ohtsuki T,Hatake K,Ikeda M,Yamada M,Motoyoshi K,Miura Y

更新日期：1996-02-01 00:00:00