Abstract:
PURPOSE:The Y402H polymorphism in the complement regulator factor H (fH) is strongly associated with age-related macular degeneration (AMD) across diverse populations. Persons homozygous for histidine at this position have up to 12-fold greater risk for AMD than those homozygous for tyrosine. Knowledge of fH-Y402H status is, therefore, valuable in predicting risk and focusing preventive measures in the elderly. This knowledge requires genetic analysis, which is unavailable in most laboratories and which provides no information about the levels of fH protein, a putative linked determinant of disease risk. METHODS:The authors describe novel monoclonal antibodies that distinguish the two fH allelic variants in plasma. ELISA with these antibodies not only reliably identifies the fH-Y402H status, confirmed by genotyping, but also quantifies the concentration of total fH and the fH-Y402 and fH-H402 variants. RESULTS:In young adult control subjects, mean fH concentration was 233 mg/L. In elderly control subjects, mean fH concentration was 269 mg/L, whereas in a matching AMD cohort, mean fH concentration was 288 mg/L. Total fH concentration was similar in each subgroup of young and elderly control subjects; however, in the AMD group, fH concentration was significantly higher in the heterozygous subgroup. Measurement of the two variants in this subgroup showed that both were elevated to a similar degree. CONCLUSIONS:The novel monoclonal antibody MBI-7 was used to develop a robust assay for measurement of fH and the variants in plasma. The simplicity of the assay means that it may be used by any clinical laboratory to identify polymorphic status and to quantify plasma levels in persons at risk for AMD.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Hakobyan S,Harris CL,Tortajada A,Goicochea de Jorge E,García-Layana A,Fernández-Robredo P,Rodríguez de Córdoba S,Morgan BPdoi
10.1167/iovs.07-1523subject
Has Abstractpub_date
2008-05-01 00:00:00pages
1983-90issue
5eissn
0146-0404issn
1552-5783pii
49/5/1983journal_volume
49pub_type
杂志文章abstract:PURPOSE:To evaluate the utility of baseline Stratus optical coherence tomography (OCT; Carl Zeiss Meditec, Dublin, CA) retinal nerve fiber layer (RNFL) normative classification in the prediction of future visual field (VF) loss. METHODS:Eighty-eight eyes with suspected glaucoma with abnormal RNFL classification by Str...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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abstract:Purpose:To investigate the molecular mechanisms of the antiangiogenic activity of UPARANT, an antagonist of the urokinase-type plasminogen activator receptor (uPAR), on primary human retinal endothelial cells (HREC) as a model of in vitro angiogenesis. Methods:The antiangiogenic activity of UPARANT was evaluated on en...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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更新日期:2011-04-14 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
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abstract::The glycosaminoglycans (GAGs) extractable from the trabecular meshworks (TM) of human and non-human primate eye have been analyzed by sequential enzymatic degradation and cellulose acetate electrophoresis. For comparison, similar extracts of the cornea, sclera, iris, and ciliary body have also been analyzed. The distr...
journal_title:Investigative ophthalmology & visual science
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doi:
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abstract:PURPOSE:To compare spectral-domain optical coherence tomography (SD-OCT) standard structural measures and a new three-dimensional (3D) volume optic nerve head (ONH) change detection method for detecting change over time in severely advanced-glaucoma (open-angle glaucoma [OAG]) patients. METHODS:Thirty-five eyes of 35 ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
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journal_title:Investigative ophthalmology & visual science
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更新日期:2009-08-01 00:00:00
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更新日期:2016-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2014-08-26 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract:PURPOSE:Subtle structural and functional neuroretinal changes have been described in human immunodeficiency virus (HIV)-infected adults without retinitis treated with combination antiretroviral therapy (cART). However, studies on this subject in HIV-infected children are scarce. This study aimed to assess the presence ...
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journal_title:Investigative ophthalmology & visual science
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doi:10.1167/iovs.06-0226
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journal_title:Investigative ophthalmology & visual science
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doi:
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更新日期:2007-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1994-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2012-09-25 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2013-07-10 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2016-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2010-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2019-12-02 00:00:00
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