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Adenylyl cyclase alpha and cAMP signaling mediate Plasmodium sporozoite apical regulated exocytosis and hepatocyte infection.

Abstract：

:Malaria starts with the infection of the liver of the host by Plasmodium sporozoites, the parasite form transmitted by infected mosquitoes. Sporozoites migrate through several hepatocytes by breaching their plasma membranes before finally infecting one with the formation of an internalization vacuole. Migration through host cells induces apical regulated exocytosis in sporozoites. Here we show that apical regulated exocytosis is induced by increases in cAMP in sporozoites of rodent (P. yoelii and P. berghei) and human (P. falciparum) Plasmodium species. We have generated P. berghei parasites deficient in adenylyl cyclase alpha (ACalpha), a gene containing regions with high homology to adenylyl cyclases. PbACalpha-deficient sporozoites do not exocytose in response to migration through host cells and present more than 50% impaired hepatocyte infectivity in vivo. These effects are specific to ACalpha, as re-introduction of ACalpha in deficient parasites resulted in complete recovery of exocytosis and infection. Our findings indicate that ACalpha and increases in cAMP levels are required for sporozoite apical regulated exocytosis, which is involved in sporozoite infection of hepatocytes.

journal_name

PLoS Pathog

journal_title

PLoS pathogens

authors

Ono T,Cabrita-Santos L,Leitao R,Bettiol E,Purcell LA,Diaz-Pulido O,Andrews LB,Tadakuma T,Bhanot P,Mota MM,Rodriguez A

doi

10.1371/journal.ppat.1000008

subject

Has Abstract

pub_date

2008-02-29 00:00:00

pages

e1000008

issue

2

eissn

1553-7366

issn

1553-7374

journal_volume

4

pub_type

杂志文章

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