Abstract:
:Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
Anderl I,Vesala L,Ihalainen TO,Vanha-Aho LM,Andó I,Rämet M,Hultmark Ddoi
10.1371/journal.ppat.1005746subject
Has Abstractpub_date
2016-07-14 00:00:00pages
e1005746issue
7eissn
1553-7366issn
1553-7374pii
PPATHOGENS-D-16-00639journal_volume
12pub_type
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