Abstract:
:Despite the high response rates of small cell lung cancer (SCLC) to first-line cisplatin-based chemotherapies, most patients with SCLC will eventually experience disease progression. Accordingly, novel chemotherapeutic regimens are desired. This in vitro study was carried out in order to develop novel chemotherapeutic regimens containing 5-fluorouracil (5-FU) or oral fluoropyrimidine for SCLC. 5-FU was combined with other standard drugs for SCLC (cisplatin, etoposide, an active metabolite of irinotecan and amrubicin) in different schedules. The combination effects were analyzed by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and an isobologram method using H69 SCLC cells. Among the examined combinations, synergistic growth inhibition was observed only when H69 cells were treated with 7-ethyl-10-hydroxycamptothecin (SN-38; an active metabolite of irinotecan) followed by 5-FU. The findings of a flow cytometric analysis were consistent with the enhancement of apoptotic cell death by this sequential treatment. This synergism was observed in 4 out of 5 SCLC cell lines tested. The effects of 5-FU and SN-38 on thymidylate synthase (TS) protein expression, an important determinant of 5-FU sensitivity, were assessed by Western blot analysis in H69 cells. Treatment with SN-38 for 24 h suppressed TS protein expression and this low level of TS was maintained for at least 72 h. Pretreatment with SN-38 inhibited the 5-FU-induced increase of TS protein. The synergistic effect induced by the combination of SN-38 and 5-FU may be attributable to the SN-38-induced suppression of TS protein. Furthermore, uracil and 5-chloro-2,4-hydroxypyridine, which are clinically available dihydropyrimidine dehydrogenase inhibitors, enhanced 5-FU-induced growth inhibition. These observations provide evidence supporting the clinical applications of the combination chemotherapy using irinotecan and 5-FU or oral fluoropyrimidines against SCLC.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Takeda K,Suyama H,Igishi T,Shigeoka Y,Matsumoto S,Yamasaki A,Hashimoto K,Sumikawa T,Morita M,Ueda Y,Shimizu Esubject
Has Abstractpub_date
2008-04-01 00:00:00pages
945-51issue
4eissn
1021-335Xissn
1791-2431journal_volume
19pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Cervical cancer is the second most commonly diagnosed cancer in women. Novel prognostic biomarkers are required to predict the progression of cervical cancer. Cervical cancer expression data were obtained from The Cancer Genome Atlas (TCGA) database. MicroRNAs (miRNAs) significantly differentially expressed between ea...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7097
更新日期:2019-06-01 00:00:00
abstract::Secreted frizzled-related protein 1 (SFRP1) is a candidate tumor suppressor gene located at 8p11.2 and antagonizes the Wnt signaling pathway. Epigenetic inactivation of SFRP1 by methylation of its promoter CpG island has recently been reported in several types of cancers. In the present study, we examined the expressi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Significance of expression of cytokine induced nitric oxide synthase (iNOS) in colorectal carcinoma remains controversial. The aim of the current study was to elucidate the significance of immunohistochemical expression of iNOS in colorectal carcinoma. The subjects were the 54 patients with colorectal carcinoma, inclu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::The multi-target kinase inhibitor sorafenib has been approved for the treatment of patients with advanced differentiated thyroid cancer. However, different sensitivities to sorafenib have been observed, and few patients have benefited from sorafenib treatment in the long term. In the event of acquired resistance to so...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.6118
更新日期:2018-02-01 00:00:00
abstract::Multidrug resistance (MDR) to chemotherapeutic drugs is the main cause of chemotherapy failure in cancer treatment, and it generally results from expression of ATP-dependent efflux pump P-glycoprotein (P-gp). MDR reversal agents typically act by inhibiting the drug efflux activity of P-gp, thereby increasing intracell...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3326
更新日期:2014-09-01 00:00:00
abstract::Migration and adhesion of tumor cells are essential prerequisites for the formation of metastases in malignant diseases. Chemokine receptor 7 (CCR7) has been shown to regulate integrin which can then facilitate adhesion of cancer cells to and/or migration through the extracellular matrix (ECM). In order to identify th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2010.989
更新日期:2010-10-01 00:00:00
abstract::Kruppel-like factor 9 (KLF9), a transcription factor, is critical for the inhibition of growth and development of tumors, whereas its effects in pancreatic cancer remains unclear. The purpose of the present study was to investigate the expression and functional significance of KLF9 in vitro, by assessing the expressio...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6760
更新日期:2018-12-01 00:00:00
abstract::Signal transducer and activator of transcription (STAT) 5, including STAT 5a and STAT 5b, was reported to play important roles in the malignant biological behaviors of tumors. However, their roles in gastric cancer, especially for STAT 5b remain unknown. This study aimed to detect the expression of STAT 5b in gastric ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3994
更新日期:2015-07-01 00:00:00
abstract::Poly(ADP-ribose) polymerase (PARP) inhibitors have been proven to represent superior clinical agents targeting DNA repair mechanisms in cancer therapy. We investigated PARP inhibitory effects of the natural and synthetic flavonoids (quercetin, rutin, monoglucosyl rutin and maltooligosyl rutin) and tested the synthetic...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2902
更新日期:2014-02-01 00:00:00
abstract::Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via di...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1585
更新日期:2012-04-01 00:00:00
abstract::Deregulation of c-Jun NH2-terminal kinase (JNK) signaling is now increasingly reported in a variety of human malignancies. Non-small cell lung cancer (NSCLC) is among such human malignancies with aberrant JNK activation; yet the exact role(s) of JNK deregulation in NSCLC biology, in particular in vivo, remains unclear...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2655
更新日期:2013-10-01 00:00:00
abstract::Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate and regulate immune responses to various antigens. DC-based vaccines have become a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is expressed at high levels in lung cancer and many other tumor cells, suggesting...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4231
更新日期:2015-11-01 00:00:00
abstract::Luteolin was recently demonstrated to suppress tumor growth by interfering with nuclear factor (NF)‑κB activation. As the NF‑κB pathway plays a critical role in muscular atrophy associated with cancer cachexia, we aimed to investigate the potential of luteolin to alleviate cancer‑associated cachexia in a Lewis lung ca...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6453
更新日期:2018-08-01 00:00:00
abstract::Neuropilin-1 (NRP-1), a co-receptor for VEGF165, is overexpressed in various prostate cancer cell lines and in advanced prostate tumors. However, distribution of the NRP-1 in prostate tumors has not yet been evaluated. Using immunohistochemical analysis, we evaluated 21 archival prostate tumors and 5 benign glands for...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::We investigated a phase III randomized trial to compare efficacy and tolerance of CDDP + 5-FU to CDDP + VP16, both given intravenously in patients with unresectable advanced head and neck cancer. The 197 eligible patients were paired off successively on the basis of tumor sites and UICC stage. Comparisons were made th...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2005-09-01 00:00:00
abstract::The aim of the present study was to evaluate human epididymis protein 4 (HE4) as a marker of epithelial ovarian cancer (EOC) relapse and the combination of this biomarker with contrast-enhanced high-resolution multidetector row computed tomography CE CT imaging to impove the monitoring of EOC patients. Twenty-one pati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2682
更新日期:2013-11-01 00:00:00
abstract::Normal breast development is controlled by a balance between cell proliferation and apoptosis. The balance between the two parameters is crucial for determining the growth or regression of breast tumours in response to therapies and treatments. Therefore, it is necessary to understand the role of apoptosis in tumour p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity pro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2452
更新日期:2013-07-01 00:00:00
abstract::Anaplastic thyroid cancer is one of the most aggressive human malignancies and is resistant to multimodal treatments. The expression of h-prune, the human homologue of Drosophila prune, has been reported to be correlated with progression and aggressiveness in various cancers including breast, colorectal and pancreatic...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4759
更新日期:2016-06-01 00:00:00
abstract::Aberrant DNA methylation and histone deacetylation participate in cancer development and progression; hence, their reversal by inhibitors of DNA methylation and histone deacetylases is a promising cancer therapy. Experimental data demonstrate that these inhibitors in combination do not only show synergy in antitumor e...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2010.1086
更新日期:2011-02-01 00:00:00
abstract::The signal transducer and activator of transcription 3 (STAT3) are ideal targets for ovarian cancer. Previous studies showed that downregulation of STAT3 using specific short hairpin RNAs (shRNA) can significantly reduce ovarian tumor growth. However, RNA interference does not fully ablate target gene expression due t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3623
更新日期:2015-02-01 00:00:00
abstract::Histone acetylation is one of the key chromatin modifications that control gene transcription during development and tumorigenesis. Recently, it was reported that the histone deacetylase inhibitor, Trichostatin A (TSA), induces growth arrest and apoptosis in tumors. However, the mol...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1496
更新日期:2012-02-01 00:00:00
abstract::Latent infection of the Epstein-Barr virus (EBV) is strongly associated with the pathogenesis of several human tumor types. The restricted expression of the latent EBV antigens is critical for EBV-associated tumors to escape from immune surveillance. EBV lytic replication can be triggered by various treatments and the...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Melanoma is the most aggressive cutaneous cancer due to its propensity to metastasise and proliferate. Melanoma accounts for 80‑90% of skin‑cancer related deaths worldwide. Alhough numerous published studies have attempted to define the markers of diagnosis and prognosis of melanoma, a sensitive and specific biomarker...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6356
更新日期:2018-06-01 00:00:00
abstract::To investigate the components of the prostaglandin biosynthetic pathway, the characteristics of cyclooxygenase (COX)-1, COX-2, hematopoietic prostaglandin D synthase (hPGDS), microsomal prostaglandin E synthase (mPGES), and thromboxane synthase (TXS) were examined for autopsied cases with small cell lung cancer (SCLC)...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Non-steroidal anti-inflammatory drugs (NSAIDs), which inhibit the enzyme cyclooxygenase (COX), are known to have a potent anti-tumorigenic activity in various cancers. However, the responsible molecular mechanisms of COX inhibition in breast cancer cells remain to be completely elucidated. We examined the effect of th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000866
更新日期:2010-08-01 00:00:00
abstract::The present study aimed to assess the in vitro and in vivo magnetic resonance imaging (MRI) features of chlorotoxin (CTX)-conjugated superparamagnetic iron oxide (SPIO) nanoprobes. CTX-conjugated nanoprobes were composed of SPIO coated with polyethylene glycol (PEG) and conjugated with CTX. The nanoprobes were termed ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4629
更新日期:2016-05-01 00:00:00
abstract::Numerous studies have suggested that increased sialylation and fucosylation levels are signs of cancer progression. The majority of studies have focused on cell surface and bloodstream glycosylation changes associated with cancer progression, while little attention has been paid to changes in the glycosylation of cyto...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2938
更新日期:2014-03-01 00:00:00
abstract::A crucial step in the path to the malignant transformation of cells and tumor formation is immortalization, which essentially depends on telomere maintenance. The aim of this study was to investigate the role of telomerase in the progression of Barrett's esophagus. Telomerase activi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2238
更新日期:2013-04-01 00:00:00
abstract::Novel agents need to be developed to overcome the limitations of the current melanoma therapeutics. Atractylenolide I (AT-I) is a sesquiterpene compound isolated from atractylodis macrocephalae rhizoma. Previous findings demonstrated that AT-I exhibited cytotoxic action in melanoma cells. However, the molecular mechan...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4111
更新日期:2015-09-01 00:00:00