当前位置： SCI文献检索 > ONCOLOGY REPORTS期刊下所有文献 > Alternative lengthening of telomeres is induced by telomerase inhibitors in Barrett's esophageal cells.

Alternative lengthening of telomeres is induced by telomerase inhibitors in Barrett's esophageal cells.

Abstract：

:A crucial step in the path to the malignant transformation of cells and tumor formation is immortalization, which essentially depends on telomere maintenance. The aim of this study was to investigate the role of telomerase in the progression of Barrett's esophagus. Telomerase activity was measured in Barrett's cells using terminal restriction fragment (TRF) analysis. Telomere length was measured using Q-FISH analysis. Furthermore, the telomere recombination events were detected between sister chromatids using chromosome orientation FISH (CO-FISH). There was a reduction in telomerase activity in the CP-A cells transduced with MT-hTER/47A+siRNA, which led to an almost complete disappearance of telomerase activity. The telomere length of the CP-A cells transduced with MT-hTER/47A+siRNA was slightly shorter compared to that of the untransduced cells. The telomerase-inhibited cells were morphologically indistinguishable from those untransduced and WT-hter-transduced cells. In the control cells, the growth rate was between 0.9 to 1.1 with the population doubling per day. Although the transduction of the telomerase inhibitors in the CP-A cells did not cause a significant reduction in cell growth, these transduced cells grew generally slower compared with the control cells. The heterogeneous telomere length was also be detected in the telomerase-inhibited CP-A cells. However, the telomere length remained homogeneous in the control cells. The metaphase of the CP-A cells transduced with MT-hTER/47A+siRNA demonstrated 70% heterogeneous telomeres. In addition, no increased recombination was observed between sister chromatids in the transduced CP-A cells compared with the control cells. Our findings suggest that an alternative lengthening of telomeres (ALT) may be induced by telomerase inhibitors in CP-A cells. Therefore, telomerase inhibitors may exhibit high potency in the treatment of esophageal adenocarcinoma arising from Barrett's esophagus.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Qin X,Qi B,Zhao B

doi

10.3892/or.2013.2238

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

1399-404

issue

eissn

1021-335X

issn

1791-2431

journal_volume

pub_type

杂志文章

在线工具

Alternative lengthening of telomeres is induced by telomerase inhibitors in Barrett's esophageal cells.