Abstract：
PURPOSE:To determine the effect of macrophage depletion on herpes simplex virus type (HAV)-1 replication in the eye and on the establishment of latency in trigeminal ganglia (TG) of immunized and ocularly infected mice. METHODS:BALB/c mice were immunized with five HSV-1 glycoprotein DNA genes or were sham immunized. The virulent HSV-1 strain KOS was used as a positive vaccine control. Immunized mice were depleted of their macrophages by dichloromethylene diphosphonate (Cl(2)MDP) injection. After ocular infection with the HSV-1 strain McKrae, virus replication in the eye, blepharitis, corneal scarring, and dermatitis were determined. Finally, the copy numbers of latency-associated transcript (LAT) and CD4(+) and CD8(+) T-cell transcripts in the TGs of surviving mice 30 days after infection were determined by RT-PCR. RESULTS:Depletion of macrophages in immunized mice increased HSV-1 replication in the eye of infected mice between days 1 and 5 after ocular infection. Depletion of macrophages did not alter the HSV-1-induced death or corneal scarring in immunized mice. Macrophage depletion, however, resulted in increased blepharitis in immunized mice. Finally, macrophage depletion had no effect on the establishment of latency in immunized mice, as the TGs from both depleted and mock-depleted mice were negative for the presence of the LAT transcript. CONCLUSIONS:In immunized mice during primary HSV-1 ocular infection, macrophages play an important role in vaccine efficacy against HSV-1 replication in the eye and blepharitis in infected mice. During the latent stage of HSV-1 infection, however, macrophage depletion failed to have any observable effect on HSV-1 latency in the TGs of infected mice.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Mott K,Brick DJ,van Rooijen N,Ghiasi Hdoi
10.1167/iovs.07-0894subject
Has Abstractpub_date
2007-12-01 00:00:00pages
5605-15issue
12eissn
0146-0404issn
1552-5783pii
48/12/5605journal_volume
48pub_type
杂志文章abstract：:Contrast sensitivity functions for sinusoidal gratings of different spatial frequencies and stimulus durations have been determined for both eyes of amblyopic subjects. For both long and short stimulus durations, the entire contrast sensitivity function of the amblyopic eyes is lower than that of the nonamblyopic eyes...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：PURPOSE:In Program K, a new automated kinetic algorithm that we developed, the frequency distributions of the number of patients' response points were obtained for external angles to distinguish normal and abnormal isopters. We also assessed the agreement between the results of Program K and Goldmann manual kinetic per...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.14-16182
更新日期：2015-03-05 00:00:00
abstract：PURPOSE:We reviewed and illustrated the most optimal retinal structural measurements to make in stem cell clinical trials. METHODS:Optical coherence tomography (OCT) and autofluorescence (AF) imaging were used to evaluate patients with severe visual loss from nonsyndromic and syndromic retinitis pigmentosa (RP), ABCA4...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.15-17654
更新日期：2016-04-01 00:00:00
abstract：PURPOSE:To investigate corneal sensitivity to selective mechanical, chemical, heat, and cold stimulation in patients with fibromyalgia (FM). METHODS:Twenty patients with FM (18 women, 2 men; 51.9 +/- 2.3 years old) and 18 control subjects (16 women, 2 men; 51.7 +/- 2.4 years) participated voluntarily in the study. Sub...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.08-3083
更新日期：2009-09-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：PURPOSE:Vasoactive intestinal peptide (VIP) is expressed by corneal endothelial (CE) cells and is present in the aqueous humor, which bathes CE cells in vivo. This study demonstrated the role of CE cell VIP in maintaining the expression level of a CE differentiation marker, N-cadherin, and the hexagonal cell shape. ME...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.07-1543
更新日期：2008-08-01 00:00:00
abstract：PURPOSE:Retinopathy of prematurity (ROP) is a major cause of visual handicap in the pediatric population. To date, this disorder is thought to stem from deficient retinal vascularization. Intriguingly, functional electrophysiological studies in patients with mild or moderate ROP and in the oxygen-induced retinopathy (O...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6742
更新日期：2011-08-05 00:00:00
abstract：PURPOSE:To investigate the accuracy and precision of threshold estimates returned by two Bayesian perimetric strategies, staircase-QUEST or SQ (a Swedish interactive threshold algorithm [SITA]-like strategy) and ZEST (zippy estimation by sequential testing), and to compare these measures with those of the full-threshol...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.03-0023
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:To develop models for assessing the differential growth of the fetal face along its horizontal and vertical axes, as well as of the orbit and the eye. METHODS:Normal human fetuses (n = 205), whose gestational ages ranged from 15.5 to 41 weeks of amenorrhea, were examined. Orbitofacial parameters were as follow...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：PURPOSE:Na,K-adenosine triphosphatase (ATPase) is essential for the regulation of cytoplasmic ion concentrations in lens cells. Earlier studies demonstrated that tyrosine phosphorylation by Lyn kinase, a Src-family member, inhibits Na,K-ATPase activity in porcine lens epithelium. In the present study, experiments were ...
journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.04-0809
更新日期：2005-02-01 00:00:00
abstract：Purpose:In patients diagnosed with Best vitelliform macular dystrophy (BVMD), quantitative fundus autofluorescence (qAF), near-infrared fundus autofluorescence (NIR-AF), and spectral-domain optical coherence tomography (SD-OCT) were used to elucidate pathogenic mechanisms. Methods:Fourteen patients heterozygous for BE...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
abstract：PURPOSE:To assess the neuroprotective effects of ketorolac administration, in solution or delivered from biodegradable microspheres, on the survival of axotomized retinal ganglion cells (RGCs). METHODS:Retinas were treated intravitreally with a single injection of tromethamine ketorolac solution and/or with ketorolac-...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.15-18213
更新日期：2016-03-01 00:00:00
abstract：:The phosphatic metabolite content of specific anatomic regions within the crystalline lens was determined by phosphorus-31 nuclear magnetic resonance analyses performed on tissue perchloric acid (PCA) extracts. Anatomically distinct zones each were dissected from five sets of 10 porcine lenses, isolated, and frozen in...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：PURPOSE:To investigate the specific role of very late antigen-1 (VLA-1; also known as integrin α1β1) in corneal inflammatory lymphangiogenesis in vivo and lymphatic endothelial cell functions in vitro. METHODS:A standard suture-induced corneal inflammatory lymphangiogenesis model was used in normal adult BALB/c mice t...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6580
更新日期：2011-07-01 00:00:00
abstract：:PURPOSE. To evaluate the shape of the posterior corneal surface in eyes with normal myopia or myopic astigmatism and the changes after LASIK and epi-LASIK. METHODS. A total of 152 eyes were included in the study. The posterior corneal elevation was measured with Scheimpflug imaging (Pentacam; Oculus, Wetzlar, Germany)...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-4811
更新日期：2010-07-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：PURPOSE:Vaccinia virus keratitis leading to blindness is a severe complication of smallpox vaccination. The clinical manifestations of vaccinia virus keratitis are similar to those of herpes simplex virus keratitis, a well-studied immunopathologic disease. Vaccinia virus keratitis is likely to involve an immunopatholog...
journal_title：Investigative ophthalmology & visual science
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更新日期：2010-09-01 00:00:00
abstract：PURPOSE:To explore whether pointwise rates of visual field progression group together in patterns consistent with retinal nerve fiber layer (RNFL) bundles. METHODS:Three hundred eighty-nine eyes of 309 patients from the Advanced Glaucoma Intervention Study with ≥6 years of follow-up and ≥12 reliable visual field exams...
journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.11-9021
更新日期：2012-04-30 00:00:00
abstract：:Electroretinographic studies of myctalopic Appaloosa horses demonstrated photopic and scotopic abnormalities similar to those in humans with congenital stationary night blindness (CSNB) of the Schubert-Bornschein type. The phototopic abnormalities consisted of reduced b-wave amplitudes and slower than normal b-wave im...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
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doi：10.1167/iovs.10-6335
更新日期：2011-04-12 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1991-09-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
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更新日期：2011-12-09 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：2001-11-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.06-0943
更新日期：2007-04-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2018-05-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：2001-05-01 00:00:00
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更新日期：2011-08-24 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2012-06-20 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
