Abstract:
:The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Rimmer S,Johnson C,Zhao B,Collier J,Gilmore L,Sabnis S,Wyman P,Sammon C,Fullwood NJ,MacNeil Sdoi
10.1016/j.biomaterials.2007.08.028subject
Has Abstractpub_date
2007-12-01 00:00:00pages
5319-31issue
35eissn
0142-9612issn
1878-5905pii
S0142-9612(07)00666-7journal_volume
28pub_type
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