Abstract:
:Understanding the biology of breast cancer stem cells and trying new ways to obliterate these cells would be a key step in developing cures for breast cancer. The objective of this study was to investigate the effect of mutant TNFalpha on human breast cancer stem cells derived from MCF7 cell line under the characterization of biologic features of these cells in vitro. By FACS analysis and sorting, we got MCF7 side population (SP) cells and showed that MCF7 SP cells possess cancer stem cell characteristics using the accepted breast cancer stem cell markers, but do not express multiple drug resistance transporters. Furthermore, by RT-PCR, these stem cells were found to constitutively express TNFR-p55 and TNFR-p75. After being treated with Mt rh471 TNFalpha, SP cells displayed a decreased self-renewal ability and an increased apoptosis by three different methods. When monocolony antibody against TNFR-p55 was added into the culture medium, the inhibitory effect of Mt rh471 TNFalpha on self-renewal was blocked completely, but this was not the case for that of Wt rhTNFalpha. The possible reasons might be that the increased binding of Mt rh471 TNFalpha mainly to TNFR-p55 results in induction of apoptosis of SP cells, while Wt rhTNFalpha could bind to both TNFR-p55 and TNFR-p75 which would lead to NFkappaB activity, resulting in a discounted apoptotic effect. These data suggest that Mt rh471 TNFalpha might be a negative regulator of the breast cancer stem cell-like cells and have the potential to treat breast cancer in clinic.
journal_name
Cancer Biol Therjournal_title
Cancer biology & therapyauthors
Li Y,Kong L,Yang Y,Li Kdoi
10.4161/cbt.6.9.4885subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1480-9issue
9eissn
1538-4047issn
1555-8576pii
4885journal_volume
6pub_type
杂志文章abstract::The hypothesis of the "liquid biopsy" using circulating tumor cells (CTCs) emerged as a minimally invasive alternative to traditional tissue biopsy to determine cancer therapy. Discordance for biomarkers expression between primary tumor tissue and circulating tumor cells (CTCs) has been widely reported, thus rendering...
journal_title:Cancer biology & therapy
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doi:10.4161/cbt.28020
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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更新日期:2012-06-01 00:00:00
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journal_title:Cancer biology & therapy
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doi:10.1080/15384047.2015.1078023
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.10.10.14010
更新日期:2010-11-15 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.11.11.15525
更新日期:2011-06-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2016.1250047
更新日期:2016-12-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章,评审
doi:10.4161/cbt.5.11.3456
更新日期:2006-11-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章,评审
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更新日期:2010-04-15 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.5.2.2318
更新日期:2006-02-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/15384047.2014.986967
更新日期:2015-01-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.22621
更新日期:2013-01-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.9.4.10744
更新日期:2010-02-01 00:00:00
abstract::SUMO (small ubiquitin-related modifier) represents a class of ubiquitin-like proteins that is conjugated, like ubiquitin, by a set of enzymes to cellular regulatory proteins, including oncogenes and tumor suppressor genes, that play key roles in the control of cell growth, differentiation and apoptosis. SUMO conjugati...
journal_title:Cancer biology & therapy
pub_type: 杂志文章,评审
doi:10.4161/cbt.74
更新日期:2002-05-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.6.12.4973
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:Unlike papillary thyroid cancer (PTC), anaplastic thyroid carcinoma (ATC) is extremely aggressive and rapidly lethal without effective therapies. However, the differences of master regulators and regulatory networks between PTC and ATC remain unclear. Methods: Three representative datasets comprising 32 ATC,...
journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2020.1803009
更新日期:2020-09-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2017.1294285
更新日期:2017-03-04 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.8.14.8882
更新日期:2009-07-01 00:00:00
abstract::AMPK has been termed the fuel sensor of mammalian cells because it directly responds to the depletion of the fuel molecule ATP. In previous work, we found that AMPK is strongly activated by tumor-like hypoxia and glucose deprivation, independently of the oxygen response system associated with HIF-1. We also observed h...
journal_title:Cancer biology & therapy
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Cancer biology & therapy
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doi:10.1080/15384047.2017.1345393
更新日期:2017-12-02 00:00:00
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doi:10.1080/15384047.2020.1856756
更新日期:2021-01-02 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-01-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.7.11.6837
更新日期:2008-11-01 00:00:00