Abstract:
:The mammalian oocyte is especially sensitive to cryopreservation. Because of its size and physiology, it can easily undergo cell death or sub-lethal damage as a consequence of intracellular ice formation, increase in the concentration of solutes and other undesired effects during the conversion of extracellular water into ice. This has generated the belief that oocyte storage cannot be achieved with the necessary efficiency and safety. However, many concerns raised by oocyte freezing are the result of unproven hypotheses or observations conducted under sometimes inappropriate conditions. For instance, spindle organization can undergo damage under certain freezing conditions but not with other protocols. The controversial suggestion that cryopreservation induces cortical granule discharge and zona pellucida hardening somehow questions the routine use of sperm microinjection. Damage to mouse oocytes caused by solute concentration is well documented but, in the human, there is no solid evidence that modifications of freezing mixtures, to prevent this problem, provide an actual advantage. The hope of developing oocyte cryopreservation as a major IVF option is becoming increasingly realistic, but major efforts are still required to clarify the authentic implications of oocyte cryopreservation at the cellular level and identify freezing conditions compatible with the preservation of viability and developmental ability.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Coticchio G,Bonu MA,Sciajno R,Sereni E,Bianchi V,Borini Adoi
10.1016/s1472-6483(10)60687-6subject
Has Abstractpub_date
2007-07-01 00:00:00pages
24-30issue
1eissn
1472-6483issn
1472-6491pii
S1472-6483(10)60687-6journal_volume
15pub_type
杂志文章,评审abstract::The discovery of cell-free fetal DNA in maternal plasma in 1997 has stimulated a rapid development of non-invasive prenatal testing. The recent advent of massively parallel sequencing has allowed the analysis of circulating cell-free fetal DNA to be performed with unprecedented sensitivity and precision. Fetal trisomi...
journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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doi:10.1016/s1472-6483(10)62183-9
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61694-x
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journal_title:Reproductive biomedicine online
pub_type: 评论,杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60111-3
更新日期:2009-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,多中心研究
doi:10.1016/s1472-6483(10)60887-5
更新日期:2007-04-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.01.013
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
pub_type: 历史文章,杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2016.07.006
更新日期:2016-10-01 00:00:00
abstract::Human embryonic stem (hES) cells are pluripotent cells isolated from early human embryos. They can be grown in vitro and made to differentiate into many different cell types. These properties have suggested that they may be useful in cell replacement therapy for many degenerative diseases. However, if hES cells could ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61801-9
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abstract::While the role of inherited thrombophilia has been accepted as a cause of recurrent late pregnancy complications, the contribution of mutated thrombophilic genes to implantation failure has not been studied. Proteins involved in fibrinolysis are necessary for trophoblast invasion into the endometrium. This study compa...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
abstract::Preimplantation genetic diagnosis and screening improves the chances of achieving a viable pregnancy, not only free of undesired single-gene defects but also aneuploidy. In addition, improvements in vitrification provide an efficient means of preserving embryos (blastocysts). By combining trophectoderm biopsy with rec...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2013.11.018
更新日期:2014-04-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.rbmo.2014.09.006
更新日期:2014-12-01 00:00:00