Abstract:
BACKGROUND:Since the osteogenic potential of bone marrow derived mesenchymal stem cells (BMSCs) becomes reduced with passage, establishment of culture condition that permit the rapid expansion of BMSCs while retaining their potential for differentiation is needed for clinical application. Bone morphogenetic proteins stimulate osteogenic differentiation in mesenchymal progenitor cells as well as increase stem cell numbers. Thus, we analyzed the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the osteogenic potential of rat BMSCs over several passages. MATERIAL AND METHODS:Osteogenic differentiation in vitro was evaluated in terms of the alkaline phosphatase (ALP) activity and the osteocalcin (OC) concentration in the supernatants, and the expression of ALP and OC mRNA in the cultured cells. For in-vivo osteogenesis, BMSCs cultured with and without rhBMP-2 through all passages were implanted into athymic mice. RESULTS:The levels of osteogenic markers were significantly higher in the cells of the BMP(+) group than in the cells of the BMP(-) group, although they decreased with passage irrespective of whether or not rhBMP-2 was added. Similar to the in-vitro experiments, there was a greater degree of bone and cartilage tissue formation in the BMP(+) group over all passages. INTERPRETATION:From our results, osteogenic potential can be maintained even in BMSCs that have been passaged several times in the presence of rhBMP-2. These cells are capable of inducing and participating in bone formation and can be used for clinical applications.
journal_name
Acta Orthopjournal_title
Acta orthopaedicaauthors
Ishikawa H,Kitoh H,Sugiura F,Ishiguro Ndoi
10.1080/17453670710013816subject
Has Abstractpub_date
2007-04-01 00:00:00pages
285-92issue
2eissn
1745-3674issn
1745-3682pii
777676841journal_volume
78pub_type
杂志文章abstract::Background and purpose - Osteoarthritis has become the most common indication for shoulder arthroplasty in Denmark, and the treatment strategies have changed towards the use of anatomical total shoulder arthroplasty and reverse shoulder arthroplasty. We investigated whether changes in the use of arthroplasty types hav...
journal_title:Acta orthopaedica
pub_type: 杂志文章,多中心研究
doi:10.1080/17453674.2019.1633759
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Following metal-on-metal hip arthroplasty (THA), immunological reactions including changes in lymphocyte populations, aseptic loosening, and lymphocytic pseudotumors occur. We hypothesized that changes in lymphocyte subpopulations would be associated with elevated metal ion concentrations. METHO...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.3109/17453674.2010.548028
更新日期:2011-02-01 00:00:00
abstract::- The International Society of Arthroplasty Registries (ISAR) Patient-Reported Outcome Measures (PROMs) Working Group have evaluated and recommended best practices in the selection, administration, and interpretation of PROMs for hip and knee arthroplasty registries. The 2 generic PROMs in common use are the Short For...
journal_title:Acta orthopaedica
pub_type: 评论,杂志文章
doi:10.1080/17453674.2016.1181816
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Degree of satisfaction with a knee arthroplasty is said to be correlated to reduced pain and better function. During a validation of the Swedish Knee Arthroplasty Register in 1997, previously operated patients were asked how satisfied they were with their knee. A subgroup of "satisfied" patients was identifi...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670510045372
更新日期:2005-12-01 00:00:00
abstract::Background and purpose - The annual number of total knee arthroplasties (TKAs) has increased worldwide in recent years. To make projections regarding future needs for primaries and revisions, additional knowledge is important. We analyzed and compared the incidences among 4 Nordic countries Patients and methods - Usin...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2016.1275200
更新日期:2017-04-01 00:00:00
abstract::Background and purpose - The uncemented Symax hip stem has shown early proximal ingrowth as result of the BONIT-hydroxyapatite (HA) coating and the distal DOTIZE surface treatment. We evaluated 2-year postoperative radiostereometric analysis (RSA) migration of the Symax hip stem in THA patients. We also investigated t...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.1080/17453674.2019.1709956
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:The occurrence of club foot (CF) varies between countries and populations, and may be related to endogenous and exogenous factors. We analyzed the occurrence of CF in a whole country over a long period of time (16 years). METHODS:Patients born in Denmark with a foot deformity 1978-93 were identified from th...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670610013114
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Most patients with femoroacetabular impingement (FAI) have a cam deformity, which may be quantified by measuring the alpha angle and anterior offset ratio (AOR). Knowledge of what constitutes a "normal" alpha angle and AOR is limited. We defined the reference intervals of these measurements from ...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453671003619011
更新日期:2010-02-01 00:00:00
abstract::Background and purpose - Information on the epidemiological trends of pelvic fractures and fracture surgery in the general population is limited. We therefore determined the incidence of pelvic fractures in the Finnish adult population between 1997 and 2014 and assessed the incidence and trends of fracture surgery.Pat...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2020.1771827
更新日期:2020-10-01 00:00:00
abstract::Background and purpose - Although the use of patient-specific positioning guides (PSPGs) in total knee replacement (TKR) in theory is promising, the technique has not yet proven its superior- ity compared with the conventional method. We compared radio- logical alignment and clinical outcome between TKR performed with...
journal_title:Acta orthopaedica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1080/17453674.2017.1393732
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:We have previously reported that 6 months of oral treatment with clodronate reduced the migration of the NexGen total knee prosthesis during the first postoperative year, as measured by radiostereometry (RSA). We now report the 4-year results. METHODS:This was a double-blind randomized study, using RSA with...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.1080/17453670610013213
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND AND PURPOSE:There is continuing confusion among practitioners with regard to the optimal choice of anesthetic type for repair of hip fractures. We investigated whether type of anesthetic was associated with short-term mortality after hip fracture surgery. PATIENTS AND METHODS:We conducted a retrospective co...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453674.2015.1128781
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:We used patient-reported outcome and risk of revision to compare hemiarthroplasty (HA) with total shoulder arthroplasty (TSA) and stemmed hemiarthroplasty (SHA) with resurfacing hemiarthroplasty (RHA) in patients with glenohumeral osteoarthritis. PATIENTS AND METHODS:We included all patients reported to the Da...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453674.2014.893497
更新日期:2014-04-01 00:00:00
abstract::Background and purpose - A lifetime perspective on revision risks is needed for optimal timing of arthroplasty in osteoarthritis (OA) patients, weighing the benefit of total hip arthroplasty/total knee arthroplasty (THA/TKA) against the risk of revision, after which outcomes are less favorable. Therefore, we provide p...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2021.1876998
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Ultrasonically driven tools have been used to reduce the incidence of complications during cement removal at revision hip replacement operations. These have been shown to be safe and effective in various ways, but produce fumes. METHODS:Using gas chromatography-mass spectroscopy, we analyzed the fumes produ...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670510041718
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:By arthroscopy, we observed a phenomenon that, according to our knowledge not previously described, we call the "biceps tendon footprint" (BTF)--an area of chondromalacia beside the bicipital groove. PATIENTS AND METHODS:We studied 118 shoulder arthroscopies prospectively. We documented whether a BTF could ...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/00016470510030634
更新日期:2005-04-01 00:00:00
abstract::Background and purpose - Socioeconomic status is associated with the outcome of major surgery. We investigated the association of socioeconomic status with the risk of early mortality and readmissions after primary total hip arthroplasty (THA). Patients and methods - We obtained information on income, education, immig...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2019.1598710
更新日期:2019-06-01 00:00:00
abstract::Background and purpose - A risk-stratification algorithm for metal-on-metal hip arthroplasty (MoMHA) patients was devised by US experts to help clinicians make management decisions. However, the proposed algorithm did not cover all potential patient or surgical abnormalities. Therefore we adapted the US risk-stratific...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2019.1659661
更新日期:2019-12-01 00:00:00
abstract:UNLABELLED:BACKGROUND AND PURPOSE Over the last 5 years, there has been increasing interest in reducing length of hospitalization (LOS) through accelerated programs. We examined the clinical outcome of patients undergoing a unicompartmental knee replacement (UKR) in an accelerated care program (A group) compared to a c...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.3109/17453670903035559
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The pathomechanics of the Essex-Lopresti lesion are not fully understood. We used human cadavers and documented the genesis of the injury with high-speed cameras. METHODS:4 formalin-fixed cadaveric specimens of human upper extremities were tested in a prototype, custom-made, drop-weight test ben...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453674.2014.887952
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:This randomized study compared clinical results after surgery for posttraumatic shoulder instability with either an anatomical repair or an older, less anatomical but commonly used method. The less anatomical procedure has been considered quicker and less demanding, but it has been questioned reg...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.3109/17453670902988345
更新日期:2009-06-01 00:00:00
abstract::Background and purpose - The treatment of patients between 55 and 70 years with displaced intracapsular femoral neck fracture remains controversial. We compared internal fixation (IF), bipolar hemiarthroplasty (HA) and total hip arthroplasty (THA) in terms of mortality, reoperations and patient-reported outcome by usi...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2017.1376514
更新日期:2018-02-01 00:00:00
abstract::Background and purpose - The Mitch proximal epiphyseal replacement (PER) was developed to preserve proximal femoral bone and minimize femoral neck fracture associated with hip resurfacing arthroplasty (HRA). We studied the survival and risk of revision of HRA compared with cementless metal-on-polyethylene (MoP) total ...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453674.2019.1646201
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Aseptic loosening is the major cause of implant failure. In cemented hip arthroplasty, failure of the acetabular side is mainly due to lysis caused by wear particles. By using an implant with low wear characteristics and by enhancing acetabular fixation using an uncemented implant, we aimed to reduce acetabu...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670710014103
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND AND PURPOSE:In recent years, intramedullary nails (INs) for the treatment of pertrochanteric hip fractures have gained prominence relative to conventional, sliding hip screws (SHSs). There is little empirical background for this development, however. A previous series of ours suggested that the use of SHS wa...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453674.2011.566143
更新日期:2011-04-01 00:00:00
abstract:BACKGROUND:Periacetabular osteotomy improves radiographic predictors of osteoarthrosis and diminishes pain and functional impairment. No changes in function quantified by gait analysis have yet been documented. We evaluated the functional outcome of periacetabular osteotomy in relation to gait. METHODS:The gait patter...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670610045920
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Several mechanisms are responsible for patellar dislocation. We investigated how the primary pathomechanism relates to patient characteristics and the outcome. METHODS:126 patients (81 females) with primary patellar dislocation reported the knee position before the episode, the movement during i...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453670903110634
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND AND PURPOSE:In recent years several different plate designs for internal fixation of fractures of the distal radius have been developed. However, few biomechanical studies have been performed to compare these new implants. The purpose of this study was to compare the mechanical properties of 5 different comm...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.3109/17453670902947440
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:Cyclooxigenase-2 (COX-2) inhibitors have been reported to delay fracture healing. To investigate the major inhibitory period of COX-2 inhibitors in fracture healing, we administrated etodolac, a COX-2-specific inhibitor, to a rat fracture model by altering the period of administration from early to late. ME...
journal_title:Acta orthopaedica
pub_type: 杂志文章
doi:10.1080/17453670510041439
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND AND PURPOSE:In unstable distal radial fractures that are impossible to reduce or to maintain in reduced position, the treatment of choice is operation. The type of operation and the choice of implant, however, is a matter of discussion. Our aim was to investigate whether open reduction and internal fixation ...
journal_title:Acta orthopaedica
pub_type: 杂志文章,随机对照试验
doi:10.3109/17453670903171875
更新日期:2009-08-01 00:00:00