The effect of recombinant human bone morphogenetic protein-2 on the osteogenic potential of rat mesenchymal stem cells after several passages.

Abstract:

BACKGROUND:Since the osteogenic potential of bone marrow derived mesenchymal stem cells (BMSCs) becomes reduced with passage, establishment of culture condition that permit the rapid expansion of BMSCs while retaining their potential for differentiation is needed for clinical application. Bone morphogenetic proteins stimulate osteogenic differentiation in mesenchymal progenitor cells as well as increase stem cell numbers. Thus, we analyzed the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the osteogenic potential of rat BMSCs over several passages. MATERIAL AND METHODS:Osteogenic differentiation in vitro was evaluated in terms of the alkaline phosphatase (ALP) activity and the osteocalcin (OC) concentration in the supernatants, and the expression of ALP and OC mRNA in the cultured cells. For in-vivo osteogenesis, BMSCs cultured with and without rhBMP-2 through all passages were implanted into athymic mice. RESULTS:The levels of osteogenic markers were significantly higher in the cells of the BMP(+) group than in the cells of the BMP(-) group, although they decreased with passage irrespective of whether or not rhBMP-2 was added. Similar to the in-vitro experiments, there was a greater degree of bone and cartilage tissue formation in the BMP(+) group over all passages. INTERPRETATION:From our results, osteogenic potential can be maintained even in BMSCs that have been passaged several times in the presence of rhBMP-2. These cells are capable of inducing and participating in bone formation and can be used for clinical applications.

journal_name

Acta Orthop

journal_title

Acta orthopaedica

authors

Ishikawa H,Kitoh H,Sugiura F,Ishiguro N

doi

10.1080/17453670710013816

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

285-92

issue

2

eissn

1745-3674

issn

1745-3682

pii

777676841

journal_volume

78

pub_type

杂志文章
  • Increased use of total shoulder arthroplasty for osteoarthritis and improved patient-reported outcome in Denmark, 2006-2015: a nationwide cohort study from the Danish Shoulder Arthroplasty Registry.

    abstract::Background and purpose - Osteoarthritis has become the most common indication for shoulder arthroplasty in Denmark, and the treatment strategies have changed towards the use of anatomical total shoulder arthroplasty and reverse shoulder arthroplasty. We investigated whether changes in the use of arthroplasty types hav...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,多中心研究

    doi:10.1080/17453674.2019.1633759

    authors: Rasmussen JV,Amundsen A,Sørensen AKB,Klausen TW,Jakobsen J,Jensen SL,Olsen BS

    更新日期:2019-10-01 00:00:00

  • Elevation of circulating HLA DR(+) CD8(+) T-cells and correlation with chromium and cobalt concentrations 6 years after metal-on-metal hip arthroplasty.

    abstract:BACKGROUND AND PURPOSE:Following metal-on-metal hip arthroplasty (THA), immunological reactions including changes in lymphocyte populations, aseptic loosening, and lymphocytic pseudotumors occur. We hypothesized that changes in lymphocyte subpopulations would be associated with elevated metal ion concentrations. METHO...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/17453674.2010.548028

    authors: Hailer NP,Blaheta RA,Dahlstrand H,Stark A

    更新日期:2011-02-01 00:00:00

  • Patient-reported outcome measures in arthroplasty registries Report of the Patient-Reported Outcome Measures Working Group of the International Society of Arthroplasty Registries Part II. Recommendations for selection, administration, and analysis.

    abstract::- The International Society of Arthroplasty Registries (ISAR) Patient-Reported Outcome Measures (PROMs) Working Group have evaluated and recommended best practices in the selection, administration, and interpretation of PROMs for hip and knee arthroplasty registries. The 2 generic PROMs in common use are the Short For...

    journal_title:Acta orthopaedica

    pub_type: 评论,杂志文章

    doi:10.1080/17453674.2016.1181816

    authors: Rolfson O,Bohm E,Franklin P,Lyman S,Denissen G,Dawson J,Dunn J,Eresian Chenok K,Dunbar M,Overgaard S,Garellick G,Lübbeke A,Patient-Reported Outcome Measures Working Group of the International Society of Arthroplasty Registries.

    更新日期:2016-07-01 00:00:00

  • Revision in previously satisfied knee arthroplasty patients is the result of their call on the physician, not on pre-planned follow-up: a retrospective study of 181 patients who underwent revision within 2 years.

    abstract:BACKGROUND:Degree of satisfaction with a knee arthroplasty is said to be correlated to reduced pain and better function. During a validation of the Swedish Knee Arthroplasty Register in 1997, previously operated patients were asked how satisfied they were with their knee. A subgroup of "satisfied" patients was identifi...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670510045372

    authors: Bremander AB,Dunbar M,Knutson K,Petersson IF,Robertsson O

    更新日期:2005-12-01 00:00:00

  • Different incidences of knee arthroplasty in the Nordic countries.

    abstract::Background and purpose - The annual number of total knee arthroplasties (TKAs) has increased worldwide in recent years. To make projections regarding future needs for primaries and revisions, additional knowledge is important. We analyzed and compared the incidences among 4 Nordic countries Patients and methods - Usin...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2016.1275200

    authors: NiemeläInen MJ,MäKelä KT,Robertsson O,W-Dahl A,Furnes O,Fenstad AM,Pedersen AB,Schrøder HM,Huhtala H,Eskelinen A

    更新日期:2017-04-01 00:00:00

  • Early stabilization of the uncemented Symax hip stem in a 2-year RSA study.

    abstract::Background and purpose - The uncemented Symax hip stem has shown early proximal ingrowth as result of the BONIT-hydroxyapatite (HA) coating and the distal DOTIZE surface treatment. We evaluated 2-year postoperative radiostereometric analysis (RSA) migration of the Symax hip stem in THA patients. We also investigated t...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.1080/17453674.2019.1709956

    authors: Kruijntjens DSMG,Koster L,Kaptein BL,Jutten LMC,Arts JJ,Ten Broeke RHM

    更新日期:2020-04-01 00:00:00

  • Increasing incidence of club foot with higher population density: incidence and geographical variation in Denmark over a 16-year period--an epidemiological study of 936,525 births.

    abstract:BACKGROUND:The occurrence of club foot (CF) varies between countries and populations, and may be related to endogenous and exogenous factors. We analyzed the occurrence of CF in a whole country over a long period of time (16 years). METHODS:Patients born in Denmark with a foot deformity 1978-93 were identified from th...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670610013114

    authors: Krogsgaard MR,Jensen PK,Kjaer I,Husted H,Lorentzen J,Hvass-Christensen B,Christensen SB,Larsen K,Sonne-Holm S

    更新日期:2006-12-01 00:00:00

  • Femoroacetabular impingement and classification of the cam deformity: the reference interval in normal hips.

    abstract:BACKGROUND AND PURPOSE:Most patients with femoroacetabular impingement (FAI) have a cam deformity, which may be quantified by measuring the alpha angle and anterior offset ratio (AOR). Knowledge of what constitutes a "normal" alpha angle and AOR is limited. We defined the reference intervals of these measurements from ...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453671003619011

    authors: Pollard TC,Villar RN,Norton MR,Fern ED,Williams MR,Simpson DJ,Murray DW,Carr AJ

    更新日期:2010-02-01 00:00:00

  • The incidence of pelvic fractures and related surgery in the Finnish adult population: a nationwide study of 33,469 patients between 1997 and 2014.

    abstract::Background and purpose - Information on the epidemiological trends of pelvic fractures and fracture surgery in the general population is limited. We therefore determined the incidence of pelvic fractures in the Finnish adult population between 1997 and 2014 and assessed the incidence and trends of fracture surgery.Pat...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2020.1771827

    authors: Rinne PP,Laitinen MK,Kannus P,Mattila VM

    更新日期:2020-10-01 00:00:00

  • No radiological and clinical advantages with patient-specific positioning guides in total knee replacement.

    abstract::Background and purpose - Although the use of patient-specific positioning guides (PSPGs) in total knee replacement (TKR) in theory is promising, the technique has not yet proven its superior- ity compared with the conventional method. We compared radio- logical alignment and clinical outcome between TKR performed with...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1080/17453674.2017.1393732

    authors: Van Leeuwen JAMJ,Snorrason F,Röhrl SM

    更新日期:2018-02-01 00:00:00

  • Postoperative clodronate decreases prosthetic migration: 4-year follow-up of a randomized radiostereometric study of 50 total knee patients.

    abstract:BACKGROUND:We have previously reported that 6 months of oral treatment with clodronate reduced the migration of the NexGen total knee prosthesis during the first postoperative year, as measured by radiostereometry (RSA). We now report the 4-year results. METHODS:This was a double-blind randomized study, using RSA with...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.1080/17453670610013213

    authors: Hilding M,Aspenberg P

    更新日期:2006-12-01 00:00:00

  • Similar mortality with general or regional anesthesia in elderly hip fracture patients.

    abstract:BACKGROUND AND PURPOSE:There is continuing confusion among practitioners with regard to the optimal choice of anesthetic type for repair of hip fractures. We investigated whether type of anesthetic was associated with short-term mortality after hip fracture surgery. PATIENTS AND METHODS:We conducted a retrospective co...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453674.2015.1128781

    authors: Brox WT,Chan PH,Cafri G,Inacio MC

    更新日期:2016-01-01 00:00:00

  • Patient-reported outcome and risk of revision after shoulder replacement for osteoarthritis. 1,209 cases from the Danish Shoulder Arthroplasty Registry, 2006-2010.

    abstract:PURPOSE:We used patient-reported outcome and risk of revision to compare hemiarthroplasty (HA) with total shoulder arthroplasty (TSA) and stemmed hemiarthroplasty (SHA) with resurfacing hemiarthroplasty (RHA) in patients with glenohumeral osteoarthritis. PATIENTS AND METHODS:We included all patients reported to the Da...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453674.2014.893497

    authors: Rasmussen JV,Polk A,Brorson S,Sørensen AK,Olsen BS

    更新日期:2014-04-01 00:00:00

  • Population-based 10-year cumulative revision risks after hip and knee arthroplasty for osteoarthritis to inform patients in clinical practice: a competing risk analysis from the Dutch Arthroplasty Register.

    abstract::Background and purpose - A lifetime perspective on revision risks is needed for optimal timing of arthroplasty in osteoarthritis (OA) patients, weighing the benefit of total hip arthroplasty/total knee arthroplasty (THA/TKA) against the risk of revision, after which outcomes are less favorable. Therefore, we provide p...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2021.1876998

    authors: Gademan MGJ,Van Steenbergen LN,Cannegieter SC,Nelissen RGHH,Marang-Van De Mheen PJ

    更新日期:2021-01-22 00:00:00

  • Gas chromatography-mass spectroscopy analysis of emissions from cement when using ultrasonically driven tools.

    abstract:BACKGROUND:Ultrasonically driven tools have been used to reduce the incidence of complications during cement removal at revision hip replacement operations. These have been shown to be safe and effective in various ways, but produce fumes. METHODS:Using gas chromatography-mass spectroscopy, we analyzed the fumes produ...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670510041718

    authors: Shewale SB,Briggs RD

    更新日期:2005-10-01 00:00:00

  • The biceps tendon footprint.

    abstract:BACKGROUND:By arthroscopy, we observed a phenomenon that, according to our knowledge not previously described, we call the "biceps tendon footprint" (BTF)--an area of chondromalacia beside the bicipital groove. PATIENTS AND METHODS:We studied 118 shoulder arthroscopies prospectively. We documented whether a BTF could ...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/00016470510030634

    authors: Sistermann R

    更新日期:2005-04-01 00:00:00

  • Increased early mortality and morbidity after total hip arthroplasty in patients with socioeconomic disadvantage: a report from the Swedish Hip Arthroplasty Register.

    abstract::Background and purpose - Socioeconomic status is associated with the outcome of major surgery. We investigated the association of socioeconomic status with the risk of early mortality and readmissions after primary total hip arthroplasty (THA). Patients and methods - We obtained information on income, education, immig...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2019.1598710

    authors: Weiss RJ,Kärrholm J,Rolfson O,Hailer NP

    更新日期:2019-06-01 00:00:00

  • Has the threshold for revision surgery for adverse reactions to metal debris changed in metal-on-metal hip arthroplasty patients? A cohort study of 239 patients using an adapted risk-stratification algorithm.

    abstract::Background and purpose - A risk-stratification algorithm for metal-on-metal hip arthroplasty (MoMHA) patients was devised by US experts to help clinicians make management decisions. However, the proposed algorithm did not cover all potential patient or surgical abnormalities. Therefore we adapted the US risk-stratific...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2019.1659661

    authors: Matharu GS,Berryman F,Dunlop DJ,Judge A,Murray DW,Pandit HG

    更新日期:2019-12-01 00:00:00

  • Similar clinical outcome after unicompartmental knee arthroplasty using a conventional or accelerated care program: a randomized, controlled study of 40 patients.

    abstract:UNLABELLED:BACKGROUND AND PURPOSE Over the last 5 years, there has been increasing interest in reducing length of hospitalization (LOS) through accelerated programs. We examined the clinical outcome of patients undergoing a unicompartmental knee replacement (UKR) in an accelerated care program (A group) compared to a c...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/17453670903035559

    authors: Borgwardt L,Zerahn B,Bliddal H,Christiansen C,Sylvest J,Borgwardt A

    更新日期:2009-06-01 00:00:00

  • Sequence of the Essex-Lopresti lesion--a high-speed video documentation and kinematic analysis.

    abstract:BACKGROUND AND PURPOSE:The pathomechanics of the Essex-Lopresti lesion are not fully understood. We used human cadavers and documented the genesis of the injury with high-speed cameras. METHODS:4 formalin-fixed cadaveric specimens of human upper extremities were tested in a prototype, custom-made, drop-weight test ben...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453674.2014.887952

    authors: Wegmann K,Engel K,Burkhart KJ,Ebinger M,Holz R,Brüggemann GP,Müller LP

    更新日期:2014-04-01 00:00:00

  • The Bankart repair versus the Putti-Platt procedure: a randomized study with WOSI score at 10-year follow-up in 62 patients.

    abstract:BACKGROUND AND PURPOSE:This randomized study compared clinical results after surgery for posttraumatic shoulder instability with either an anatomical repair or an older, less anatomical but commonly used method. The less anatomical procedure has been considered quicker and less demanding, but it has been questioned reg...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/17453670902988345

    authors: Salomonsson B,Abbaszadegan H,Revay S,Lillkrona U

    更新日期:2009-06-01 00:00:00

  • High failure rate after internal fixation and beneficial outcome after arthroplasty in treatment of displaced femoral neck fractures in patients between 55 and 70 years.

    abstract::Background and purpose - The treatment of patients between 55 and 70 years with displaced intracapsular femoral neck fracture remains controversial. We compared internal fixation (IF), bipolar hemiarthroplasty (HA) and total hip arthroplasty (THA) in terms of mortality, reoperations and patient-reported outcome by usi...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2017.1376514

    authors: Bartels S,Gjertsen JE,Frihagen F,Rogmark C,Utvåg SE

    更新日期:2018-02-01 00:00:00

  • Survival and revision causes of hip resurfacing arthroplasty and the Mitch proximal epiphyseal replacement: results from the Danish Hip Arthroplasty Register.

    abstract::Background and purpose - The Mitch proximal epiphyseal replacement (PER) was developed to preserve proximal femoral bone and minimize femoral neck fracture associated with hip resurfacing arthroplasty (HRA). We studied the survival and risk of revision of HRA compared with cementless metal-on-polyethylene (MoP) total ...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453674.2019.1646201

    authors: Tang-Jensen M,Kjærsgaard-Andersen P,Poulsen TK,Overgaard S,Varnum C

    更新日期:2019-12-01 00:00:00

  • Uncemented metal-on-metal acetabular component: follow-up of 112 hips for a minimum of 5 years.

    abstract:BACKGROUND:Aseptic loosening is the major cause of implant failure. In cemented hip arthroplasty, failure of the acetabular side is mainly due to lysis caused by wear particles. By using an implant with low wear characteristics and by enhancing acetabular fixation using an uncemented implant, we aimed to reduce acetabu...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670710014103

    authors: Vassan UT,Sharma S,Chowdary KP,Bhamra MS

    更新日期:2007-08-01 00:00:00

  • Intramedullary nailing appears to be superior in pertrochanteric hip fractures with a detached greater trochanter: 311 consecutive patients followed for 1 year.

    abstract:BACKGROUND AND PURPOSE:In recent years, intramedullary nails (INs) for the treatment of pertrochanteric hip fractures have gained prominence relative to conventional, sliding hip screws (SHSs). There is little empirical background for this development, however. A previous series of ours suggested that the use of SHS wa...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453674.2011.566143

    authors: Palm H,Lysén C,Krasheninnikoff M,Holck K,Jacobsen S,Gebuhr P

    更新日期:2011-04-01 00:00:00

  • Walking pattern in 9 women with hip dysplasia 18 months after periacetabular osteotomy.

    abstract:BACKGROUND:Periacetabular osteotomy improves radiographic predictors of osteoarthrosis and diminishes pain and functional impairment. No changes in function quantified by gait analysis have yet been documented. We evaluated the functional outcome of periacetabular osteotomy in relation to gait. METHODS:The gait patter...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670610045920

    authors: Pedersen EN,Alkjaer T,Søballe K,Simonsen EB

    更新日期:2006-04-01 00:00:00

  • The mechanism of primary patellar dislocation: trauma history of 126 patients.

    abstract:BACKGROUND AND PURPOSE:Several mechanisms are responsible for patellar dislocation. We investigated how the primary pathomechanism relates to patient characteristics and the outcome. METHODS:126 patients (81 females) with primary patellar dislocation reported the knee position before the episode, the movement during i...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453670903110634

    authors: Nikku R,Nietosvaara Y,Aalto K,Kallio PE

    更新日期:2009-08-01 00:00:00

  • Different osteosyntheses for Colles' fracture: a mechanical study in 42 cadaver bones.

    abstract:BACKGROUND AND PURPOSE:In recent years several different plate designs for internal fixation of fractures of the distal radius have been developed. However, few biomechanical studies have been performed to compare these new implants. The purpose of this study was to compare the mechanical properties of 5 different comm...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.3109/17453670902947440

    authors: Krukhaug Y,Gjerdet NR,Lundberg OJ,Lilleng PK,Hove LM

    更新日期:2009-04-01 00:00:00

  • Cyclooxygenase-2 inhibitor delays fracture healing in rats.

    abstract:BACKGROUND:Cyclooxigenase-2 (COX-2) inhibitors have been reported to delay fracture healing. To investigate the major inhibitory period of COX-2 inhibitors in fracture healing, we administrated etodolac, a COX-2-specific inhibitor, to a rat fracture model by altering the period of administration from early to late. ME...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章

    doi:10.1080/17453670510041439

    authors: Endo K,Sairyo K,Komatsubara S,Sasa T,Egawa H,Ogawa T,Yonekura D,Murakami R,Yasui N

    更新日期:2005-08-01 00:00:00

  • Open reduction and internal fixation compared to closed reduction and external fixation in distal radial fractures: a randomized study of 50 patients.

    abstract:BACKGROUND AND PURPOSE:In unstable distal radial fractures that are impossible to reduce or to maintain in reduced position, the treatment of choice is operation. The type of operation and the choice of implant, however, is a matter of discussion. Our aim was to investigate whether open reduction and internal fixation ...

    journal_title:Acta orthopaedica

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/17453670903171875

    authors: Abramo A,Kopylov P,Geijer M,Tägil M

    更新日期:2009-08-01 00:00:00