Abstract:
AIM:To develop a conditionally replicative gene-viral vector system called CNHK500-p53, which contains dual promoters within the E1 region, and combines the advantages of oncolytic virus and gene therapies for hepatocellular carcinoma (HCC). METHODS:CNHK500-p53 was constructed by using human telomerase reverse transcriptase (hTERT) promoter to drive adenovirus E1a gene and hypoxia response element (HRE) promoter to drive adenovirus E1b gene. p53 gene expressing cassette was inserted into the genome of replicative virus. Viral replication experiments, cytopathic effect (CPE) and methyl thiazolyl tetrazolium (MTT) assay were performed to test the selective replication and oncolytic efficacy of CNHK500-p53. RESULTS:Immunohistochemistry verified that infection with CNHK500-p53 was associated with selective replication of adenovirus and production of p53 protein in telomerase-positive and hypoxia-inducible factor-dependent HCC cells. p53 protein secreted from HepG2, infected with CNHK500-p53 was significantly higher than that infected with nonreplicative adenovirus Ad-p53 in vitro (388 +/- 34.6 microg/L vs 76.3 +/- 13.17 microg/L). Viral replication experiments showed that replication of CNHK500-p53 and CNHK500 or WtAd5, was much stronger than that of Ad-p53 in tested HCC cell lines. CPE and MTT assay indicated that CNHK500-p53 selectively replicated in and killed HCC cells while leaving normal cells unaffected. CONCLUSION:A more efficient gene-viral system is developed by combining selective oncolysis with exogenous expression of p53 against HCC cells.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zhao HC,Zhang Q,Yang Y,Lu MQ,Li H,Xu C,Chen GHdoi
10.3748/wjg.v13.i5.683subject
Has Abstractpub_date
2007-02-07 00:00:00pages
683-91issue
5eissn
1007-9327issn
2219-2840journal_volume
13pub_type
杂志文章abstract:AIM:To describe the learning curves of hand-assisted laparoscopic D2 radical gastrectomy (HALG) for the treatment of gastric cancer. METHODS:The HALG surgical procedure consists of three stages: surgery under direct vision via the port for hand assistance, hand-assisted laparoscopic surgery, and gastrointestinal tract...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i5.1606
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abstract:AIM:To explore the expression of macrophage inflammatory protein-1alpha (MIP-1alpha) in Kupffer cells (KCs) following liver ischemia/reperfusion injury IRI in rats. METHODS:Forty male SD rats were divided randomly into five groups. A model of partial warm ischemia/reperfusion injury in the rat liver was established. K...
journal_title:World journal of gastroenterology
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doi:10.3748/wjg.v12.i24.3854
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abstract:AIM:To investigate the effect of antiviral therapy with nucleoside analogs in hepatitis B virus (HBV)-related cirrhosis and esophageal varices. METHODS:Eligible patients with HBV-related cirrhosis and esophageal varices who consulted two tertiary hospitals in Beijing, China, the Chinese Second Artillery General Hospit...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
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更新日期:2013-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i44.7061
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journal_title:World journal of gastroenterology
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journal_title:World journal of gastroenterology
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doi:10.3748/wjg.v18.i29.3875
更新日期:2012-08-07 00:00:00
abstract:AIM:To retrospectively evaluate the prognosis of patients with hepatocellular carcinoma (HCC) with or without a history of therapy for HCC following transcatheter arterial embolization (TAE). METHODS:One hundred and twenty-one patients with HCC treated with TAE from 1992 to 2004 in our hospital were enrolled in this s...
journal_title:World journal of gastroenterology
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更新日期:2006-04-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i46.7497
更新日期:2006-12-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2019-12-07 00:00:00
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journal_title:World journal of gastroenterology
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i37.5867
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2012-11-14 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v16.i27.3445
更新日期:2010-07-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i35.4776
更新日期:2007-09-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2004-09-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i7.1060
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journal_title:World journal of gastroenterology
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pub_type: 杂志文章
doi:10.3748/wjg.v24.i3.379
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v19.i7.1005
更新日期:2013-02-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2014-03-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i3.375
更新日期:2013-01-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i19.2707
更新日期:2007-05-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i24.7529
更新日期:2015-06-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2012-09-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2015-10-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2011-06-28 00:00:00