Abstract:
BACKGROUND:Flagellin subunit A gene (flaA) typing of Campylobacter has been recognized by several groups as a relatively simple and quick genotyping method. The present study aimed to create, for the first time in Greece, a database with flaA restriction patterns, which could be used for future epidemiological and clinical studies. A total of 207 C. jejuni clinical isolates of known serotype were collected from 5 general hospitals of the area of Attica, during the period 2000-2003. RESULTS:The RFLP profiles of each strain were matched in 44 bins of 0 or 1. Thirty nine different flaA types, designated as flaA 1 GR to flaA 39 GR (GR: Greece) were found. There was no significant association of certain genotypes with certain serotypes. However flaA typing showed a remarkable discriminatory ability inside the non-typable (NT) group. CONCLUSIONS:Evaluating our results we observed (i) that there was no clonality of a certain flaA type among the strains and the serotypes examined and (ii) that the discriminatory ability of flaA typing was much better than that of serotyping. Giving a simple and detailed description of the data analysis, we are the first who publish the bin patterns for the flaA genotypes found.
journal_name
Eur J Epidemioljournal_title
European journal of epidemiologyauthors
Ioannidis A,Nicolaou C,Legakis NJ,Ioannidou V,Chatzipanagiotou Sdoi
10.1007/s10654-006-9073-2subject
Has Abstractpub_date
2006-01-01 00:00:00pages
823-9issue
11eissn
0393-2990issn
1573-7284journal_volume
21pub_type
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