Abstract：
:Endocrinological research on wild animals inhabiting remote areas has been hampered by the need to store plasma samples at subzero temperatures. In an attempt to remedy this logistical issue, we here investigate the use of ethanol as an alternative to freezing for the preservation of steroid and indoleamine hormones in avian plasma. Known quantities of the steroids 5alpha-dihydrotestosterone (DHT), testosterone, 17beta-estradiol, corticosterone, and the indoleamine melatonin were added to a stripped pool of chicken plasma. Samples were either immediately frozen at -40 degrees C, or treated with pure ethanol. Ethanol-treated samples were either immediately frozen, or-to simulate storage conditions at various field locations-left sitting at room temperature for one to two months, or incubated at 36 degrees C for one month before all treatment groups were frozen at -40 degrees C. All samples were then analyzed by radioimmunoassay. For DHT and estradiol there were no differences among treatment groups suggesting that ethanol-treatment is as effective as immediate freezing in preserving plasma steroid concentrations. For testosterone, corticosterone and melatonin ethanol-treated samples differed significantly from immediately frozen samples suggesting that caution is needed when comparing absolute concentrations of hormones between samples preserved in different ways. However, differences among ethanol-treated samples in general were small, demonstrating the feasibility of this preservation method in the field at remote locations.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Goymann W,Schwabl I,Trappschuh M,Hau Mdoi
10.1016/j.ygcen.2006.09.014subject
Has Abstractpub_date
2007-01-15 00:00:00pages
191-5issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(06)00307-8journal_volume
150pub_type
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journal_title：General and comparative endocrinology
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更新日期：1983-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1008
更新日期：1994-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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更新日期：2004-06-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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更新日期：2013-09-01 00:00:00
abstract：:The aim of the study was to verify the effect of various hormonal agents [human chorionic gonadotropin (hCG) and salmon gonadoliberine analogue (sGnRHa)] applied at different stages of maturity of the females [out-of-season (maturation stage I) and in-season spawning (maturation stages II and IV)] on the proximate com...
journal_title：General and comparative endocrinology
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更新日期：2017-05-01 00:00:00
abstract：:Expression of the tilapia prolactin receptor (tiPRL-R) has been characterized in the intestine of Oreochromis niloticus and the levels of both tiPRL-R transcripts and tiPRL binding sites have been further analyzed in this organ, as well as in gill and kidney, during adaptation of tilapia to a hyperosmotic environment....
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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