Inhibitory effect of ethylene glycol monoethyl ether on rat sperm motion.

Abstract:

:Ethylene glycol monoethyl ether (EGEE) is known to have testicular toxicity. To elucidate whether EGEE has any effect on sperm motion, especially in the case of short time exposure, we conducted a series of in vivo experiments with rats, as well as an in vitro study with rat sperm. Sperm from cauda epididymides and spermaducts was analyzed for the change in motion with a Hamilton-Thorne Sperm analyzer. Administration of EGEE at 600 mg/kg/d for five weeks significantly decreased total and progressive motility of sperm to 15-30% of controls, in both the cauda epididymis and the spermaduct. The time-course experiment using a single dose of 1,000 mg/kg showed that damage to sperm motion was evident at 12-24 h after EGEE administration. Addition of EGEE to the medium of sperm had no effect on its motion, but the metabolite ethoxyacetic acid showed a significant inhibitory effect. These results suggest that besides its toxicity to spermatogenesis, the metabolite of EGEE may also directly affect the motion of mature sperm.

journal_name

Ind Health

journal_title

Industrial health

authors

Wang RS,Ohtani K,Suda M,Nakajima T

doi

10.2486/indhealth.44.665

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

665-8

issue

4

eissn

0019-8366

issn

1880-8026

pii

JST.JSTAGE/indhealth/44.665

journal_volume

44

pub_type

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