Abstract：
:The aim of this study was to provide preliminary validation of a new sublingual mucosal cell line (HO-1-u-1) for use as in vitro sublingual drug delivery screening of compounds involving passive diffusion. HO-1-u-1 cells were seeded on cell culture inserts. The ultrastructure and integrity of cell layers, inter-passage variation and directionality of drug transport, and apparent permeability coefficient (P(app)) of eight beta-blockers (representing compounds involving passive diffusion) were determined. HO-1-u-1 cells grown on inserts formed stratified and epithelial-like structure and maintained the typical histological features of normal human sublingual epithelium. The maximal integrity of the cell layer was reached in 23 days. No significant inter-passage variation was found at the passages ranging from 2 to 11 when measured by radiolabeled transcellular and paracellular markers (testosterone and mannitol, respectively). Bidirectional transport studies confirmed the passive diffusion as the mechanism of transport for these markers. The P(app) of eight beta-blockers across HO-1-u-1 cell culture ranged from 2.89+/-0.17 to 6.37+/-0.37x10(-6)cm/s and correlated well to the P(app) obtained from porcine sublingual mucosa (r(2)=0.647 and 0.83 when excluding propranolol). The above results indicate that the HO-1-u-1 cells grown on inserts may offer as a potentially in vitro model for screening sublingual drug permeation involving passive diffusion.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Wang Y,Zuo Z,Lee KK,Chow MSdoi
10.1016/j.ijpharm.2006.10.012subject
Has Abstractpub_date
2007-04-04 00:00:00pages
27-34issue
1-2eissn
0378-5173issn
1873-3476pii
S0378-5173(06)00850-7journal_volume
334pub_type
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2006.02.015
更新日期：2006-06-06 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2006.10.043
更新日期：2007-04-20 00:00:00
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更新日期：2018-12-20 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2016.06.130
更新日期：2016-09-10 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2006.02.028
更新日期：2006-06-19 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2002-12-05 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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更新日期：2003-03-18 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2005-01-20 00:00:00
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journal_title：International journal of pharmaceutics
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更新日期：2015-01-01 00:00:00
abstract：:Electrospun nanofibers are being utilized for a wide range of healthcare applications. A plethora of natural and synthetic polymers are exploited for their ability to be electrospun and replace the complex habitat provided by the extracellular matrix for the cells. The fabrication of nanofibers can be tuned to act as ...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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更新日期：2017-05-15 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2014-03-25 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 临床试验,杂志文章
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更新日期：2019-05-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2016-09-10 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2017.05.052
更新日期：2017-11-30 00:00:00
