Abstract：
:Curiosity surrounding the physiological relevance of neural insulin signaling has gradually developed since the discovery that nervous tissue contains both the hormone and its receptor. Similar to other receptor tyrosine kinases, ligand interaction with the insulin receptor (IR) activates a variety of intracellular signaling pathways, particularly those relevant to cellular survival. Consequently, one explanation for the presence of the insulin pathway in the brain may involve participation in the response to neuronal injury. To investigate this possibility, the present study began by examining the effect of oxygen-glucose deprivation (OGD), a well-characterized in vitro model of ischemia, on ligand-binding, surface expression, and function of the IR in cultured rat neurons that were prepared under serum-free conditions. Reduced insulin-binding was observed following OGD, although surface expression of the receptor was not altered. However, OGD did significantly decrease the ability of insulin to stimulate phosphorylation of the transmembrane IR beta-subunit, without affecting protein expression of this subunit. Subsequent experiments focused on the manner in which pharmacologically manipulating IR function affected neuronal viability after OGD. Application of the IR sensitizer metformin moderately improved neuronal viability, while the specific IR tyrosine kinase inhibitor tyrphostin A47 was able to dramatically decrease viability; both compounds acted without affecting IR surface expression. Our study suggests that not only does the IR appear to play an important role in neuronal survival, but also that neurons may actively maintain IRs on the cell surface to compensate for the OGD-induced decrease in the ability of insulin to phosphorylate its receptor.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Mielke JG,Taghibiglou C,Wang YTdoi
10.1016/j.neuroscience.2006.07.055subject
Has Abstractpub_date
2006-11-17 00:00:00pages
165-73issue
1eissn
0306-4522issn
1873-7544pii
S0306-4522(06)01061-Xjournal_volume
143pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:Clinical reports show that oxytocin (OT) is related to stress-related disorders such as depression, anxiety disorder, and post-traumatic stress disorder. Two key structures in the brain should be paid special attention with regard to stress regulation, namely, the hypothalamus and the hippocampus. The former is the re...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2019.07.046
更新日期：2019-10-01 00:00:00
abstract：:Deafferentation of the adult rat dentate gyrus induces reactive axonal growth by surviving afferent systems. In middle aged and aged rats, axonal sprouting is delayed and reduced relative to young adults. The cause for this age-related decline is not known, but it may reflect a decrement in trophic signals which initi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00539-3
更新日期：1998-04-01 00:00:00
abstract：:We tested a hypothesis that the classical relation between movement time and index of difficulty (ID) in quick pointing action (Fitts' Law) reflects processes at the level of motor planning. Healthy subjects stood on a force platform and performed quick and accurate hand movements into targets of different size locate...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.11.043
更新日期：2013-02-12 00:00:00
abstract：:In the present study, we investigated whether endoplasmic reticulum (ER) stress is associated with neuronal- and astroglial-death in the hippocampus using LiCl-pilocarpine-induced status epilepticus (SE) rat model. Glucose-related protein (GRP) 78 and protein disulfide isomerase (PDI) expressions were transiently incr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.08.061
更新日期：2015-10-29 00:00:00
abstract：:NESP55 (neuroendocrine secretory protein of M(r) 55000) is a novel member of the chromogranin family. In the present study, we have investigated the distribution, axonal transport and proteolytic processing of NESP55 in the peripheral nervous system. The amount of NESP55 immunoreactivity in adrenal gland was more than...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(01)00465-1
更新日期：2002-01-01 00:00:00
abstract：:Intracellular recordings were performed from projection cells of the cat dorsal lateral geniculate nucleus in vitro to investigate the properties and origin of optic tract evoked inhibitory postsynaptic potentials mediated by GABAB receptors and their relationship to the physiologically different cell classes present ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90307-2
更新日期：1989-01-01 00:00:00
abstract：:The dual effect of capsaicin on primary afferent neurons, excitation and stimulation of transmitter release, its dependence on extracellular calcium and its modulation by Ruthenium Red have been investigated in the rabbit ear. Injection of capsaicin into the central artery of the isolated perfused ear with intact neur...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90124-3
更新日期：1989-01-01 00:00:00
abstract：:The Tg2576 mouse model of Alzheimer's disease (AD) exhibits age-dependent amyloid beta (Abeta) deposition in the brain. We studied electroencephalographically defined sleep and the circadian regulation of waking activities in Tg2576 mice to determine whether these animals exhibit sleep abnormalities akin to those in A...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.11.018
更新日期：2005-01-01 00:00:00
abstract：:We sought to determine the impact of 17beta-estradiol throughout the hippocampal trisynaptic pathway and to investigate the afferent fiber systems within CA1 and CA3 in detail. To achieve this objective, we utilized multielectrode arrays to simultaneously record the field excitatory postsynaptic potentials from the CA...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.03.075
更新日期：2006-08-11 00:00:00
abstract：:Neuropathic pain resulting from spinal cord injury is often accompanied by maladaptive plasticity of the central nervous system, including the opioid receptor-rich periaqueductal gray (PAG). Evidence suggests that sensory signaling via the PAG is robustly modulated by dopamine D1- and D2-like receptors, but the effect...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.11.040
更新日期：2017-02-20 00:00:00
abstract：:In the nervous system, the aminoacylhistidine dipeptide carnosine (beta-alanyl-L-histidine) has been shown to be expressed in the olfactory receptor neurons and in brain astrocytes. Using immunocytochemical techniques, we report here a dense carnosine-like immunoreactivity in the subependymal layer of the rodent foreb...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00596-4
更新日期：1998-07-01 00:00:00
abstract：:Mutations in the presenilin 1 and 2 genes cause the majority of early onset familial forms of Alzheimer's disease. Here we describe the biochemical and immunohistochemical characterization of calsenilin, a novel calcium binding protein that we have previously shown to interact with presenilins 1 and 2, in mouse brain....
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00251-8
更新日期：2002-01-01 00:00:00
abstract：:The glucocorticoid-induced receptor (GIR) is an orphan G-protein-coupled receptor awaiting pharmacological characterization. GIR was originally identified in murine thymoma cells, and shows a widespread, yet not completely complementary distribution in mouse and human brain. Expression of the mouse GIR gene is modulat...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.01.066
更新日期：2005-01-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate whether an objective measure of freezing of gait (FOG) using a validated alternating stepping in place (SIP) task, is related to executive and/or visuospatial cognitive impairment in Parkinson's disease (PD). METHODS:We studied prospectively 30 PD subjects with the U...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.07.007
更新日期：2012-09-27 00:00:00
abstract：:Lesioning of thalamic target neurons in the adult rat provokes a resorption of terminal axonal branches of afferent neurons from the dorsal column nuclei. There is, however, no massive neuronal loss in the dorsal column nuclei. In the adult, therefore, the thalamic post synaptic target cells influence primarily the ex...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90348-4
更新日期：1994-10-01 00:00:00
abstract：:Alzheimer's disease (AD), the most prominent form of dementia in elderly, is a yet incurable degenerative neurological illness characterized by memory loss. Here, we used an AD rat model to investigate the in vivo efficacy of caprospinol, a disease-modifying steroid developed on the concept that reduced synthesis of 2...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.10.033
更新日期：2010-01-20 00:00:00
abstract：:Previous studies have suggested that peptides such as substance P and thyrotropin-releasing hormone coexist with serotonin in the same varicosities in the ventral horn and intermediate gray of the spinal cord in rat. However, coexistence of these peptides with serotonin is rare in fibers in the superficial dorsal horn...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90212-k
更新日期：1992-10-01 00:00:00
abstract：:Our current knowledge of the sites of action of the centrally-acting antihypertensive drug clonidine is based almost entirely on experiments in anesthetized animals. The aim of this study was to determine, in conscious rabbits, the sites of action in the brainstem of systemically administered clonidine, as well as its...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)00583-q
更新日期：1995-05-01 00:00:00
abstract：:We have recently shown that isolated neuronal growth cones from developing rat forebrain possess an appreciable activity of adenylate cyclase, producing cyclic adenosine monophosphate, which can be stimulated by various neurotransmitter receptor agonists and by forskolin [Lockerbie R. O., Hervé D., Blanc G., Tassin J....
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90191-7
更新日期：1989-01-01 00:00:00
abstract：:Previous studies have clearly demonstrated that the immediate-early gene, c-fos can regulate, through its protein product Fos, the expression of the pro-opiomelanocortin gene. In the present study, immunohistochemistry for Fos and beta-endorphin was used to assess the basal activity of hypothalamic pro-opiomelanocorti...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00208-0
更新日期：1999-01-01 00:00:00
abstract：:Using spinal cord and dorsal root ganglion cell cultures, we have studied the immuno-histochemical distribution of several peptide products of the dynorphin gene. With antibody directed toward the midregion of dynorphin A, peptide-immunoreactivity was found exclusively in the cell bodies of spinal cord neurons. Antibo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(86)90110-7
更新日期：1986-08-01 00:00:00
abstract：:In order to gain insights into the steps (binding, uptake, intracellular effect) which differ in the inhibitory actions of tetanus toxin and botulinum neurotoxins types A or B, their temperature dependencies were investigated at identified cholinergic and non-cholinergic synapses in Aplysia. Upon lowering the temperat...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00336-3
更新日期：1996-01-01 00:00:00
abstract：:Development and differentiation of basal forebrain-derived cholinergic neurons were studied using a new technique that combines dissociated cell cultures with organotypic slice cultures. Slices of cerebral cortex or entire forebrain hemispheres were taken from early postnatal rat pups and maintained as organotypic cul...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00067-5
更新日期：1997-10-01 00:00:00
abstract：:The expression pattern of the pannexin2 protein (Px2) in healthy and ischemized brains of adult rats was investigated. A polyclonal antibody for rat Px2 was generated in chicken and purified for affinity. This antibody was used to study by Western blot, Enzyme-Linked Immunosorbent Assay, and immunohistochemistry, the ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.06.028
更新日期：2007-09-07 00:00:00
abstract：:Neurotransmitter release and changes in the concentration of intracellular free calcium ([Ca++]i) were studied in cultured GABAergic cerebral cortical neurons, from mice, upon depolarization with either an unphysiologically high potassium concentration (55 mM) or the physiological excitatory neurotransmitter glutamate...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(93)90592-4
更新日期：1993-06-01 00:00:00
abstract：:The organization of the claustrocortical system was investigated in rat by means of cortical injections of either lectin-conjugated horseradish peroxidase or retrograde fluorescent tracers. The latter were also employed in cat. Evans Blue, Fast Blue, True Blue, Nuclear Yellow and Diamidino Yellow were used in differen...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(85)90192-7
更新日期：1985-11-01 00:00:00
abstract：:Biochemical and immunocytochemical analyses were performed to resolve how glutamate and glutamine are handled in rat cerebellar cortex in acute hypoosmotic stress. Rats were subjected to a 15-20% reduction in plasma osmolality by intraperitoneal injection of distilled water and then perfusion fixed after 4 or 8 h surv...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(96)00003-6
更新日期：1996-06-01 00:00:00
abstract：:The aim of this study was to determine whether forebrain neurons projecting to the mediodorsal nucleus of the thalamus in rats express glutamate decarboxylase messenger RNA as a marker for GABAergic neurons. Forebrain glutamate decarboxylase messenger RNA-containing neurons that project to the mediodorsal nucleus were...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00351-i
更新日期：1996-01-01 00:00:00
abstract：:Adult synapsin triple-knockout mice exhibit epilepsy that manifests as generalized tonic-clonic seizures. Because in vitro recordings have shown a reduction in quantal release from inhibitory neurons, an inherent excitation-inhibition imbalance has been hypothesized as the direct culprit for epilepsy in these mice. We...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.05.030
更新日期：2011-08-25 00:00:00
abstract：:Peripheral and central diabetic neuropathies were studied in streptozotocin-diabetic rats, using behavioral, biochemical and electrophysiological techniques. Diabetic rats showed thermal hypoalgesia and decreasing motor nerve conduction velocity at 4 and 8 weeks of diabetes. In addition, amplitude of the evoked potent...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.02.047
更新日期：2009-05-05 00:00:00
