Abstract:
:The surface plasmon resonance (SPR) approach, being a relatively novel biophysical method, is used to detect many different targets by biomolecular interaction. The SPR system uses optical and evanescent wave phenomenon. This approach does not need any labels, such as enzymes or isotopes, and the monitored interactions are in real time. In DNA-DNA interaction, the SPR approach is Tm-independent. Here we report our preliminary results for the molecular detection of the Hb S (GAG -->GTG) mutation at codon 6 of the human beta-globin gene. Our preliminary results show that the SPR approach could be applied as an inexpensive and fast routine test system for the molecular diagnosis of abnormal hemoglobins (Hbs), especially in premarital screening programs.
journal_name
Hemoglobinjournal_title
Hemoglobinauthors
Atalay EO,Ustel E,Yildiz S,Atalay Adoi
10.1080/03630260600755807subject
Has Abstractpub_date
2006-01-01 00:00:00pages
385-91issue
3eissn
0363-0269issn
1532-432Xpii
H670669U7J633U72journal_volume
30pub_type
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