Abstract:
:A chromatographic procedure for the separation of human globin chains is described. This method uses CM-Sepharose CL-6B as ion-exchanger and NaCl gradients formed in sodium phosphate buffers containing urea and 0.05M mercaptoethanol to elute the chains. One advantage of this system is that a column, once packed, may be used repeatedly for several chain separations, thus reducing cost and practical work. The resolution in our hands was also better than that on CM-Cellulose using phosphate buffer gradients.
journal_name
Hemoglobinjournal_title
Hemoglobinauthors
Sparham SJ,Huehns ERdoi
10.3109/03630267909069151subject
Has Abstractpub_date
1979-01-01 00:00:00pages
13-20issue
1eissn
0363-0269issn
1532-432Xjournal_volume
3pub_type
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