Abstract:
:To study effects of ionizing radiation on apoptosis induction in leukocytes of alpha-thalassemia (alpha-thal) variants compared to normal controls, venous blood samples were obtained from 10 healthy volunteers and 30 alpha-thal patients. Different types alpha-thal were diagnosed by multiplex polymerase chain reaction (PCR). Blood samples were irradiated with three Gy gamma rays, used for comet assay, immediately or 48 h after irradiation. Results show that the frequency of background as well as apoptosis in silent alpha-thal carriers, alpha-thal carriers and controls was similar but there was a significant difference between Hb H patients and other groups in the study. The increased apoptosis in Hb H patients might suggest that accumulation of beta-globin and oxidative stresses are effective in causing apoptosis, and cells from these patients are more vulnerable to damage from radiation-induced toxic substances. Therefore, from alpha-thal patients, those with Hb H disease might be considered as radiosensitive in terms of apoptosis formation.
journal_name
Hemoglobinjournal_title
Hemoglobinauthors
Tarang A,Mozdarani H,Akbari MTdoi
10.1080/03630260903039586subject
Has Abstractpub_date
2009-01-01 00:00:00pages
247-57issue
3eissn
0363-0269issn
1532-432Xpii
913567112journal_volume
33pub_type
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